Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.

abstract：:Rapid identification of pathogens from blood cultures can decrease lengths of stay and improve patient outcomes. We evaluated the accuracy of the Verigene Gram-positive blood culture (BC-GP) nucleic acid test for investigational use only (Nanosphere, Inc., Northbrook, IL) for the identification of Gram-positive bacter...

journal_title：Journal of clinical microbiology

authors： Wojewoda CM,Sercia L,Navas M,Tuohy M,Wilson D,Hall GS,Procop GW,Richter SS

更新日期：2013-07-01 00:00:00

abstract：:Several media were used to evaluate the hemolytic activity of Listeria monocytogenes, and this property was used along with the API Listeria system to identify Listeria spp. All L. monocytogenes strains were identified correctly with this system, and blood agar base no. 2 and Columbia blood agar base supplemented with...

journal_title：Journal of clinical microbiology

authors： Fujisawa T,Mori M

更新日期：1994-04-01 00:00:00

abstract：:The emergence of azole-resistant strains of Aspergillus fumigatus during treatment for aspergillosis occurs by a mutation selection process. Understanding how antifungal resistance mechanisms evolve in the host environment during infection is of great clinical importance and biological interest. Here, we used next-gen...

journal_title：Journal of clinical microbiology

authors： Hagiwara D,Takahashi H,Watanabe A,Takahashi-Nakaguchi A,Kawamoto S,Kamei K,Gonoi T

更新日期：2014-12-01 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996 and affected more t...

journal_title：Journal of clinical microbiology

authors： Mitsuda T,Muto T,Yamada M,Kobayashi N,Toba M,Aihara Y,Ito A,Yokota S

更新日期：1998-03-01 00:00:00

abstract：:Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [C...

journal_title：Journal of clinical microbiology

authors： Lepine DG,Neumann PW,Frenette SL,O'Shaughnessy MV

更新日期：1990-06-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:The genus Corynebacterium is a heterogeneous group of species comprising human and animal pathogens and environmental bacteria. It is defined on the basis of several phenotypic characters and the results of DNA-DNA relatedness and, more recently, 16S rRNA gene sequencing. However, the 16S rRNA gene is not polymorphic ...

journal_title：Journal of clinical microbiology

authors： Khamis A,Raoult D,La Scola B

更新日期：2004-09-01 00:00:00

abstract：:To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as indicated by the abse...

journal_title：Journal of clinical microbiology

authors： Schoeb TR,Dybvig K,Keisling KF,Davidson MK,Davis JK

更新日期：1997-07-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:Anyplex STI-7 is a new molecular kit that detects seven sexually transmitted pathogens. Among 202 subjects screened for genital infection, 143 (70.4%) were diagnosed with at least one pathogen, in concordance with reference methods. In addition, the Anyplex STI-7 demonstrated coinfections, such as that with Ureaplasma...

journal_title：Journal of clinical microbiology

authors： Berçot B,Amarsy R,Goubard A,Aparicio C,Loeung HU,Segouin C,Gueret D,Jacquier H,Meunier F,Mougari F,Cambau E

更新日期：2015-03-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection. While most serologic assays are qualitativ...

journal_title：Journal of clinical microbiology

authors： Higgins V,Fabros A,Kulasingam V

更新日期：2021-01-22 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:The heterogeneity and the increasing clinical importance of the Enterobacter cloacae complex have often been discussed. However, little is known about molecular factors causing pathogenicity within this nomenspecies. Here, we analyzed the genetic differences between an avirulent plant isolate and a pathogenic strain c...

journal_title：Journal of clinical microbiology

authors： Kremer AN,Hoffmann H

更新日期：2012-10-01 00:00:00

abstract：:Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

journal_title：Journal of clinical microbiology

authors： Mills EL,Rholl KS,Quie PG

更新日期：1980-07-01 00:00:00

abstract：:Human group C rotavirus was identified in central Australia in each of eight years over a 16-year period between 1982 and 1997. Cases occurred either sporadically but over a relatively short period of time or as clustered outbreaks. These are the only reports of human group C rotavirus in Australia other than that of ...

journal_title：Journal of clinical microbiology

authors： Schnagl RD,Boniface K,Cardwell P,McCarthy D,Ondracek C,Coulson B,Erlich J,Morey F

更新日期：2004-05-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:The continuous spread of community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) and the introduction of these highly virulent isolates into hospitals represent increasing threats. The timely recognition of caMRSA strains is crucial for infection control purposes. Thus, we developed a PCR-based assay f...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Braulke C,Pasemann B,Schmidt C,Witte W

更新日期：2008-02-01 00:00:00

abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

journal_title：Journal of clinical microbiology

authors： Kerremans JJ,Verbrugh HA,Vos MC

更新日期：2012-06-01 00:00:00

abstract：:The use of different assay conditions has complicated the evaluation of studies relating salivary lysozyme levels to oral or systemic disease. The purpose of this study was to compare values obtained for lysozyme activity in mixed saliva of 104 healthy subjects by using two assay techniques and four variations in samp...

journal_title：Journal of clinical microbiology

authors： Jenzano JW,Hogan SL,Lundblad RL

更新日期：1986-12-01 00:00:00

abstract：:The BBL Crystal system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a miniaturized bacterial identification method employing modified conventional and chromogenic substrates. Two products are currently available, the Rapid Stool/Enteric ID Kit and the Enteric/Nonfermenter ID Kit, each comprising thirt...

journal_title：Journal of clinical microbiology

authors： Holmes B,Costas M,Thaker T,Stevens M

更新日期：1994-09-01 00:00:00

abstract：:We compared the use of bacteriophage sensitivity, seroagglutination with polyclonal antisera raised in rabbits or horses, seroagglutination with murine monoclonal antibody, and the serum agar precipitin technique for the detection of K1 capsular polysaccharide among clinical isolates of Escherichia coli obtained from ...

journal_title：Journal of clinical microbiology

authors： Cross A,Orskov I,Orskov F,Sadoff J,Gemski P

更新日期：1984-08-01 00:00:00

abstract：:Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...

journal_title：Journal of clinical microbiology

authors： Barman P,Ghosh S,Das S,Varghese V,Chaudhuri S,Sarkar S,Krishnan T,Bhattacharya SK,Chakrabarti A,Kobayashi N,Naik TN

更新日期：2004-06-01 00:00:00

abstract：:Brucellosis is a globally significant zoonosis, the control of which is difficult and resource intensive. Serological tests form a vital part of a multifactorial approach to control and are often performed in large numbers. The aim of the present study was to develop a new assay to improve the efficiency, ease, and ef...

journal_title：Journal of clinical microbiology

authors： McGiven JA,Thompson IJ,Commander NJ,Stack JA

更新日期：2009-10-01 00:00:00

abstract：:A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

journal_title：Journal of clinical microbiology

authors： Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

更新日期：2017-12-01 00:00:00

abstract：:The purpose of the present study was to determine the extent to which bacteria not detected by culture contribute to the microbial flora of the bowel of preterm infants with and without neonatal necrotizing enterocolitis (NEC). Fecal samples from 32 preterm infants in special care baby units including samples from 10 ...

journal_title：Journal of clinical microbiology

authors： Millar MR,Linton CJ,Cade A,Glancy D,Hall M,Jalal H

更新日期：1996-10-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:Because of its low yield in unselected specimens, stool culture is often cost ineffective. We tested 55 fecal samples from Fairfax Hospital (46 patients with diarrhea and 9 from controls without diarrhea) for lactoferrin by latex agglutination (LFLA) with the Leukotest (Techlab, Blacksburg, Va.) as a marker for inflam...

journal_title：Journal of clinical microbiology

authors： Choi SW,Park CH,Silva TM,Zaenker EI,Guerrant RL

更新日期：1996-04-01 00:00:00

abstract：:The zygomycetous mold Cokeromyces recurvatus was isolated from the pleural and peritoneal fluids of a 64-year-old man with a history of peptic ulcer disease and alcohol abuse. The patient presented to the hospital with severe abdominal pain and a ruptured duodenal ulcer. This is one of the few times that C.recurvatus ...

journal_title：Journal of clinical microbiology

authors： Munipalli B,Rinaldi MG,Greenberg SB

更新日期：1996-10-01 00:00:00