Evaluation of media for determining hemolytic activity and that of API Listeria system for identifying strains of Listeria monocytogenes.

abstract：:We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 9...

journal_title：Journal of clinical microbiology

authors： Shin BM,Mun SJ,Yoo SJ,Kuak EY

更新日期：2012-11-01 00:00:00

abstract：:While testing the in vitro activities of 14 antimicrobial agents against 107 methicillin-susceptible Staphylococcus aureus (MSSA) and 250 methicillin-resistant S. aureus (MRSA) isolates collected in The Netherlands, we found to our surprise that 19 (7.6%) MRSA isolates were suspected of having reduced susceptibilities...

journal_title：Journal of clinical microbiology

authors： Van Griethuysen A,Van 't Veen A,Buiting A,Walsh T,Kluytmans J

更新日期：2003-06-01 00:00:00

abstract：:A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

journal_title：Journal of clinical microbiology

authors： Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

更新日期：2006-01-01 00:00:00

abstract：:An immunoblot assay for the serological diagnosis of Helicobacter pylori infection was evaluated. Serum samples from patients whose gastric biopsy specimens were known to be positive or negative for H. pylori on culture were used to establish interpretive criteria for the immunoblot assay. A panel of sera from patient...

journal_title：Journal of clinical microbiology

authors： Nilsson I,Ljungh A,Aleljung P,Wadström T

更新日期：1997-02-01 00:00:00

abstract：:The activity of three aminoglycosides and six beta-lactam antibiotics on strains of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and enterococci was studied. The minimal inhibitory concentrations (MICs), the minimal bactericidal concentrations (MBCs), and t...

journal_title：Journal of clinical microbiology

authors： Lorian V,Atkinson BA

更新日期：1982-07-01 00:00:00

abstract：:Campylobacter fetus subsp. jejuni was isolated from the feces of 14.8% of a random sample of the healthy population of rural southern India. The rate of isolation was highest in preschool children. This finding emphasizes the need to identify markers of pathogenicity in strains of C. fetus subsp. jejuni, which so far ...

journal_title：Journal of clinical microbiology

authors： Rajan DP,Mathan VI

更新日期：1982-05-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on a major surface protein 5 (MSP5) B-cell epitope conserved among Anaplasma species was used to detect goats infected with Anaplasma ovis. We examined strains of A. ovis isolated from goats in Kenya and demonstrated that MSP5 and the target B-ce...

journal_title：Journal of clinical microbiology

authors： Ndung'u LW,Aguirre C,Rurangirwa FR,McElwain TF,McGuire TC,Knowles DP,Palmer GH

更新日期：1995-03-01 00:00:00

abstract：:A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

journal_title：Journal of clinical microbiology

authors： Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

更新日期：2014-07-01 00:00:00

abstract：:A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

journal_title：Journal of clinical microbiology

authors： Shimizu M,Shirai J,Narita M,Yamane T

更新日期：1990-02-01 00:00:00

abstract：:After repeated use of fluconazole for therapy of oropharyngeal candidosis, the emergence of in vitro fluconazole-resistant Candida albicans isolates (MIC, > or = 25 micrograms/ml) together with oral candidosis unresponsive to oral dosages of up to 400 mg of fluconazole were observed in patients with human immunodefici...

journal_title：Journal of clinical microbiology

authors： Ruhnke M,Eigler A,Tennagen I,Geiseler B,Engelmann E,Trautmann M

更新日期：1994-09-01 00:00:00

abstract：:The specific identification of verocytotoxin (VT)-producing Escherichia coli (VTEC) requires the detection of VTs in bacterial culture filtrates or the detection of genes encoding these toxins in bacterial cells by specific DNA probes. The standard method for detecting these toxins involves a time-consuming, labor-int...

journal_title：Journal of clinical microbiology

authors： Basta M,Karmali M,Lingwood C

更新日期：1989-07-01 00:00:00

abstract：:Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containi...

journal_title：Journal of clinical microbiology

authors： Reimer LG,Reller LB

更新日期：1985-02-01 00:00:00

abstract：:In spite of the excellent performance of rapid tuberculosis (TB) nucleic acid amplification (NAA) tests and the clear benefits of immediate diagnosis of TB disease, NAA tests frequently are not used in the diagnosis of pulmonary TB cases, particularly TB cases with smear-negative sputa. Public health laboratories prim...

journal_title：Journal of clinical microbiology

authors： Han LL,Elvin P,Bernardo J

更新日期：2012-08-01 00:00:00

abstract：:White-footed mice, Peromyscus leucopus, were captured in southern Connecticut during 1997 and 1998 to determine the prevalence of infections caused by granulocytic Ehrlichia sp., Borrelia burgdorferi, and Babesia microti. Of the 50 mice captured and recaptured, 25 of 47 (53.2%) and 23 of 48 (47.9%) contained antibodie...

journal_title：Journal of clinical microbiology

authors： Stafford KC 3rd,Massung RF,Magnarelli LA,Ijdo JW,Anderson JF

更新日期：1999-09-01 00:00:00

abstract：:Mycobacterium abscessus, which consists of the two subspecies M. abscessus subspecies abscessus and M. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. Here we analyzed 50 M. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (...

journal_title：Journal of clinical microbiology

authors： Rüger K,Hampel A,Billig S,Rücker N,Suerbaum S,Bange FC

更新日期：2014-01-01 00:00:00

abstract：:The findings from a preliminary assessment of a new instrument designed for the inoculation and spreading of specimens for microbiological analysis onto agar plates are described. The study found that the instrument was able to select full or biplates from a number of input cassettes, each containing different agar ty...

journal_title：Journal of clinical microbiology

authors： Glasson JH,Guthrie LH,Nielsen DJ,Bethell FA

更新日期：2008-04-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:A rapid flow cytometric assay for in vitro antifungal drug susceptibility testing was developed by adapting the proposed reference method for broth macrodilution testing of yeasts. Membrane permeability changes caused by the antifungal agent were measured by flow cytometry using propidium iodide, a nucleic acid-bindin...

journal_title：Journal of clinical microbiology

authors： Ramani R,Ramani A,Wong SJ

更新日期：1997-09-01 00:00:00

abstract：:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcera...

journal_title：Journal of clinical microbiology

authors： Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel G

更新日期：2010-10-01 00:00:00

abstract：:Typing and in vitro cultivation of rotavirus-positive human stool samples stored unintentionally at ambient tropical temperatures for 2 1/2 months showed that rotavirus is stable and may still be infectious in vitro. This indicates that stool specimen collection for rotavirus studies can be performed in areas and sett...

journal_title：Journal of clinical microbiology

authors： Fischer TK,Steinsland H,Valentiner-Branth P

更新日期：2002-12-01 00:00:00

abstract：:Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, aft...

journal_title：Journal of clinical microbiology

authors： Bouscambert-Duchamp M,Lina B,Trompette A,Moret H,Motte J,Andréoletti L

更新日期：2005-03-01 00:00:00

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...

journal_title：Journal of clinical microbiology

authors： Farber JM,Speirs JI

更新日期：1987-08-01 00:00:00

abstract：:A heminested inverse PCR (HIP) for the amplification of sequences flanking the Mycobacterium tuberculosis insertion sequence IS6110 has been developed. The method depends upon primers that anneal to IS6110 at sites between its 5' end and the closest BsrFI site. The accuracy of HIP was demonstrated by the amplification...

journal_title：Journal of clinical microbiology

authors： Patel S,Wall S,Saunders NA

更新日期：1996-07-01 00:00:00

abstract：:In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensi...

journal_title：Journal of clinical microbiology

authors： Mommert S,Gutzmer R,Kapp A,Werfel T

更新日期：2001-07-01 00:00:00

abstract：:We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain ...

journal_title：Journal of clinical microbiology

authors： Rosa PA,Hogan D,Schwan TG

更新日期：1991-03-01 00:00:00

abstract：:The babA2 and cagA genes were investigated in 208 Brazilian Helicobacter pylori strains. A strong association between babA2 and duodenal ulcer or gastric carcinoma was observed, even after adjusting for confounding factors, such as age, gender, and cagA status. cagA-positive strains were also independently associated ...

journal_title：Journal of clinical microbiology

authors： Oliveira AG,Santos A,Guerra JB,Rocha GA,Rocha AM,Oliveira CA,Cabral MM,Nogueira AM,Queiroz DM

更新日期：2003-08-01 00:00:00

abstract：:The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and ...

journal_title：Journal of clinical microbiology

authors： Bruun FN,McGarrity GJ,Blakemore WS,Coriell LL

更新日期：1976-03-01 00:00:00

abstract：:Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

journal_title：Journal of clinical microbiology

authors： Mexas AM,Hancock SI,Breitschwerdt EB

更新日期：2002-12-01 00:00:00