Abstract:
:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcerative lesions in cases where scarred edges prevent the collection of swabs.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel Gdoi
10.1128/JCM.01549-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
3732-4issue
10eissn
0095-1137issn
1098-660Xpii
JCM.01549-10journal_volume
48pub_type
杂志文章abstract::In this study, we evaluated a rapid whole-blood test, BM-test Helicobacter pylori, for detection of H. pylori infection in 144 and 48 patients with other gastrointestinal symptoms and with gastric cancer, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic a...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:10.1128/JCM.35.10.2695-2697.1997
更新日期:1997-10-01 00:00:00
abstract::The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 sto...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.8.3835-3839.2005
更新日期:2005-08-01 00:00:00
abstract::Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.9.2381-2386.1993
更新日期:1993-09-01 00:00:00
abstract::The BACTEC 9240 (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) is a new continuous-monitoring blood culture system that uses internal, fluorescent-CO2 sensors. In a multicenter clinical trial, organism yield and times to detection with the prototype BACTEC 9240 system were compared with those of the BAC...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1128/JCM.31.3.552-557.1993
更新日期:1993-03-01 00:00:00
abstract::Spiroplasma species are organisms that normally colonize plants and insects. We describe the first case of human systemic infection caused by Spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. Spiroplasma turonicum was identified th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02841-14
更新日期:2015-02-01 00:00:00
abstract::Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 1...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00848-20
更新日期:2020-09-22 00:00:00
abstract::The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Hepatitis E virus (HEV) is responsible for a majority of sporadic and epidemic viral hepatitides in India and other developing countries. Even though the genomes of four geographically distinct strains of HEV have been cloned and sequenced, the Indian strain of HEV remains largely uncharacterized. We have cloned and s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.10.2653-2659.1995
更新日期:1995-10-01 00:00:00
abstract::The accessory gene regulator (agr) is a central system that controls the expression of Staphylococcus aureus virulence factors. In this study, the distribution of agr alleles, defined by agr restriction fragment length polymorphism within agr interference groups, among S. aureus isolates from bovine and human origin w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.42.3.1265-1269.2004
更新日期:2004-03-01 00:00:00
abstract::We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,meta分析
doi:10.1128/JCM.42.10.4566-4576.2004
更新日期:2004-10-01 00:00:00
abstract::The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmission is overestima...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00489-13
更新日期:2013-07-01 00:00:00
abstract::The clinical performance of the cobas human papillomavirus (HPV) test for detection of high-grade disease in a colposcopy-referred population was compared with that of Hybrid Capture 2 (HC2). The overall agreement between the tests was 92.3%. Clinical sensitivity and specificity for detection of cervical intraepitheli...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01949-13
更新日期:2013-10-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) in northern Alberta was detected using two enzyme immunoassays and an in-house real-time PCR. Of 2,328 stool samples, 8 were positive for O157:H7 STEC and 13 were positive for non-O157 STEC. No significant gender (P = 0.17) or age (P = 0.81) differences between groups were...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.05211-11
更新日期:2011-12-01 00:00:00
abstract::The taxonomic status and structure of Streptococcus dysgalactiae have been the object of much confusion. Bacteria belonging to this species are usually referred to as Lancefield group C or group G streptococci in clinical settings in spite of the fact that these terms lack precision and prevent recognition of the exac...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.05900-11
更新日期:2012-01-01 00:00:00
abstract::The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.1.28-31.1994
更新日期:1994-01-01 00:00:00
abstract::We report a case of catheter-related bloodstream infection by Tsukamurella inchonensis, identified using 16S rRNA gene sequencing, in a patient with myelofibrosis who underwent a bone marrow transplant. Tsukamurella species infections are rare. To our knowledge, this is the first case of T. inchonensis bloodstream inf...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00421-14
更新日期:2014-06-01 00:00:00
abstract::Eight commercial enzyme-linked immunosorbent assays (ELISAs) were used to test sera taken from 102 patients in whom Helicobacter pylori infection status had been determined by means of biopsy culture, PCR, histology, and urease production and by 13C urea breath test. By those means, 61 patients had been found to be in...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.1.292-294.1997
更新日期:1997-01-01 00:00:00
abstract::Anyplex STI-7 is a new molecular kit that detects seven sexually transmitted pathogens. Among 202 subjects screened for genital infection, 143 (70.4%) were diagnosed with at least one pathogen, in concordance with reference methods. In addition, the Anyplex STI-7 demonstrated coinfections, such as that with Ureaplasma...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.03370-14
更新日期:2015-03-01 00:00:00
abstract::PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00770-09
更新日期:2009-08-01 00:00:00
abstract::Cell-free and cell-associated human immunodeficiency virus cultures suspended in 10% serum remained infectious for several weeks at room temperature. The stability was further increased when cell-associated virus was suspended in neat serum. When dried onto a glass coverslip, virus remained infectious for several days...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.2.571-574.1994
更新日期:1994-02-01 00:00:00
abstract::This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here proc...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged storage at room tempera...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00717-06
更新日期:2006-11-01 00:00:00
abstract::Clostridium difficile is the most common cause of health care-associated diarrhea. Accurate and rapid diagnosis is essential to improve patient outcome and prevent disease spread. We compared our two-step diagnostic algorithm, an enzyme immunoassay for glutamate dehydrogenase (GDH) followed by the cytotoxin neutraliza...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01648-10
更新日期:2010-12-01 00:00:00
abstract::Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.10.5327-5331.2005
更新日期:2005-10-01 00:00:00
abstract::The contamination of semiclosed disposable circuits of Healthdyne and Bourns ventilators was studied in a newborn intensive care unit over a 2-year period. A total of 379 fluid samples was obtained from inspiratory and expiratory tubing condensates and traps and from thermal humidifier columns fed with prefilled conta...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.2.322-328.1986
更新日期:1986-02-01 00:00:00
abstract::From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00699-06
更新日期:2006-10-01 00:00:00
abstract::Group A streptococci and enterococci were identified by two L-pyrrolidonyl-beta-naphthylamide (PYR) hydrolysis tests. One uses Minitek PYR disks (BBL Microbiology Systems, Cockeysville, Md.); the other uses a swab impregnated with PYR reagent. Both tests and Strep-A-Chek (EY Laboratories, San Mateo, Calif.), a commerc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.9.1805-1806.1987
更新日期:1987-09-01 00:00:00
abstract::The beta and gamma genera of papillomaviruses consist of epidermodysplasia verruciformis-related human papillomaviruses (HPVs) and phylogenetically related cutaneous HPVs. Here, we have developed a consensus primer PCR assay and reverse line blot typing system coupled thereto (referred to as beta and gamma cutaneous H...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.11.5581-5587.2005
更新日期:2005-11-01 00:00:00
abstract::We compared a direct fluorescent-antibody stain (DFA) and an enzyme immunoassay (EIA) with a standard cell culture technique for the detection of Chlamydia trachomatis infection in women in an urban family practice setting. We also evaluated a DFA sample in a commercial laboratory to determine the interlaboratory reli...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.7.1580-1585.1990
更新日期:1990-07-01 00:00:00
abstract::We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.3.524-532.1991
更新日期:1991-03-01 00:00:00