An Indian strain of hepatitis E virus (HEV): cloning, sequence, and expression of structural region and antibody responses in sera from individuals from an area of high-level HEV endemicity.

abstract：:The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Bernard CN

更新日期：2000-09-01 00:00:00

abstract：:We evaluated the performance of the DR. TBDR/NTM IVD kit, which was designed to detect Mycobacterium tuberculosis, rifampin-resistant M. tuberculosis, and nontuberculous mycobacteria, for detecting 110 positive and 50 negative cultures in Mycobacterium Growth Indicator Tubes. The accuracy rate of this kit for identifi...

journal_title：Journal of clinical microbiology

authors： Lee MR,Cheng A,Huang YT,Liu CY,Chung KP,Wang HC,Liang SK,Liao CH,Yu CJ,Hsueh PR

更新日期：2012-10-01 00:00:00

abstract：:Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...

journal_title：Journal of clinical microbiology

authors： Queiroz A,Sousa R,Heine C,Cardoso M,Guimarães LH,Machado PR,Carvalho EM,Riley LW,Wilson ME,Schriefer A

更新日期：2012-12-01 00:00:00

abstract：:Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in t...

journal_title：Journal of clinical microbiology

authors： Li H,Dyer N,Keller J,Crawford TB

更新日期：2000-04-01 00:00:00

abstract：:A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reasses...

journal_title：Journal of clinical microbiology

authors： Kondas AV,Olson VA,Li Y,Abel J,Laker M,Rose L,Wilkins K,Turner J,Kline R,Damon IK

更新日期：2015-04-01 00:00:00

abstract：:The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase ...

journal_title：Journal of clinical microbiology

authors： Rüsch-Gerdes S,Pfyffer GE,Casal M,Chadwick M,Siddiqi S

更新日期：2006-03-01 00:00:00

abstract：:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase,...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1983-10-01 00:00:00

abstract：:The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with s...

journal_title：Journal of clinical microbiology

authors： Edner N,Castillo-Rodas P,Falk L,Hedman K,Söderlund-Venermo M,Allander T

更新日期：2012-02-01 00:00:00

abstract：:In 1998, an outbreak of systemic infections caused by Bacillus cereus occurred in the Neonatal Intensive Care Unit of the University Hospital Vrije Universiteit, Amsterdam, The Netherlands. Three neonates developed sepsis with positive blood cultures. One neonate died, and the other two neonates recovered. An environm...

journal_title：Journal of clinical microbiology

authors： Van Der Zwet WC,Parlevliet GA,Savelkoul PH,Stoof J,Kaiser AM,Van Furth AM,Vandenbroucke-Grauls CM

更新日期：2000-11-01 00:00:00

abstract：:Among 23 patients carrying methicillin-resistant Staphylococcus aureus (MRSA) in their anterior nares, 6 (26%) also carried methicillin-susceptible S. aureus (MSSA) as less prevalent flora. In 4 of the 6 patients, the MSSA was unrelated to prevalent MRSA, as determined by pulsed-field gel electrophoresis (PFGE), multi...

journal_title：Journal of clinical microbiology

authors： Boundy S,Zhao Q,Fairbanks C,Folgosa L,Climo M,Archer GL

更新日期：2012-02-01 00:00:00

abstract：:We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were...

journal_title：Journal of clinical microbiology

authors： Niedrig M,Schmitz H,Becker S,Günther S,ter Meulen J,Meyer H,Ellerbrok H,Nitsche A,Gelderblom HR,Drosten C

更新日期：2004-04-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:In five separate fecal collections spanning three years, group A rotaviruses were detected by enzyme-linked immunosorbent assay in 35 (25%) of 142 specimens obtained from nondiarrheic, hospitalized neonates in Blantyre, Malawi. Molecular characterization of each strain identified, for the first time in neonates, a sho...

journal_title：Journal of clinical microbiology

authors： Cunliffe NA,Rogerson S,Dove W,Thindwa BD,Greensill J,Kirkwood CD,Broadhead RL,Hart CA

更新日期：2002-04-01 00:00:00

abstract：:Herpes simplex virus type 2 (HSV-2) seroprevalence in 68 patients with AIDS was 77% by Western blot (immunoblot) and 44% by glycoprotein G-2 immunoassay. Each of 16 patients with culture-proven HSV-2 infection was positive by Western blot versus 8 by glycoprotein G-2 immunoassay. No differences in age, race, duration ...

journal_title：Journal of clinical microbiology

authors： Safrin S,Arvin A,Mills J,Ashley R

更新日期：1992-05-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglu...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Thulliez P,Desmonts G,Remington JS

更新日期：1988-05-01 00:00:00

abstract：:Campylobacter fetus subsp. fetus was isolated from the stools of two homosexual males. One was asymptomatic at the time of isolation. The other presented with diarrhea. Both isolates were initially grown at 42 degrees C. This organism should be included among the list of organisms that are found in homosexual males. ...

journal_title：Journal of clinical microbiology

authors： Devlin HR,McIntyre L

更新日期：1983-10-01 00:00:00

abstract：:In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

journal_title：Journal of clinical microbiology

authors： Harris MC,Deuber C,Polin RA,Nachamkin I

更新日期：1989-10-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the ...

journal_title：Journal of clinical microbiology

authors： Clem TR,Yolken RH

更新日期：1978-01-01 00:00:00

abstract：:Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine t...

journal_title：Journal of clinical microbiology

authors： Kohl TA,Utpatel C,Niemann S,Moser I

更新日期：2018-08-27 00:00:00

abstract：:The Autobac IDX system (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.) for rapid, semiautomated identification of gram-negative bacilli was compared with the identification methods in routine use in four laboratories. The study included 1,515 organisms representing 30 species of enteric and nonenteric b...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Matsen JM,Morello JA,Smith PB,Tilton RC

更新日期：1984-04-01 00:00:00

abstract：:The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

journal_title：Journal of clinical microbiology

authors： Woods GL,LaTemple D,Cruz C

更新日期：1994-04-01 00:00:00

abstract：:Nosocomial transmission of Neisseria meningitidis has only rarely been reported. Here, we present a significant spatiotemporal association of two cases of invasive meningococcal disease identified by retrospective cluster analysis with the program SaTScan. The most likely epidemiological link was simultaneous hospital...

journal_title：Journal of clinical microbiology

authors： Elias J,Health Office in the Rural District Office Wartburgkreis.,Claus H,Frosch M,Vogel U

更新日期：2006-11-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimi...

journal_title：Journal of clinical microbiology

authors： Rockland M,Ruth MM,Aalders N,Pennings L,Hoefsloot W,Wattenberg M,van Ingen J

更新日期：2019-03-28 00:00:00

abstract：:Fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedosporium prolificans isolates. A total of 59 arbitrary primers were screened with six unrelated S. prolificans isolates, and a panel of 12 primers was selected. The 12 primers were then used to detect DNA polymorphisms a...

journal_title：Journal of clinical microbiology

authors： San Millán R,Quindós G,Garaizar J,Salesa R,Guarro J,Pontón J

更新日期：1997-09-01 00:00:00

abstract：:We have evaluated the KOH test, the antibiotic disk identification test, and the Gram stain reaction for the preliminary grouping of gram-positive and gram-negative anaerobes and have assessed the value of erythromycin 60-micrograms-disk resistance as a predictive index of clindamycin resistance among anaerobes. By te...

journal_title：Journal of clinical microbiology

authors： Bourgault AM,Lamothe F

更新日期：1988-10-01 00:00:00