Campylobacter fetus subsp. fetus in homosexual males.

abstract：:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

journal_title：Journal of clinical microbiology

authors： Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

更新日期：2010-03-01 00:00:00

abstract：:Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

journal_title：Journal of clinical microbiology

authors： Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

更新日期：2003-06-01 00:00:00

abstract：:In a follow-up study, enterotoxigenic Escherichia coli (ETEC) infections in 145 children from two communities located in northeastern Argentina were monitored for 2 years. The occurrence of diarrhea was monitored by weekly household visits. Of 730 fecal specimens collected, 137 (19%) corresponded to diarrheal episodes...

journal_title：Journal of clinical microbiology

authors： Viboud GI,Jouve MJ,Binsztein N,Vergara M,Rivas M,Quiroga M,Svennerholm AM

更新日期：1999-09-01 00:00:00

abstract：:Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

journal_title：Journal of clinical microbiology

authors： Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

更新日期：2003-12-01 00:00:00

abstract：:Nucleic acid sequence capture extraction was coupled with LightCycler PCR amplification and product detection using real-time fluorescence for rapid, definitive detection of Mycobacterium bovis in lymph node specimens from 38 cattle with bovine tuberculosis lesions. PCR amplification of sequence-captured DNA using bot...

journal_title：Journal of clinical microbiology

authors： Taylor MJ,Hughes MS,Skuce RA,Neill SD

更新日期：2001-04-01 00:00:00

abstract：:We tested 16,191 strains of Candida against posaconazole and voriconazole, using the CLSI M27-A3 broth microdilution (BMD) method (24-h incubation), in order to define wild-type (WT) populations and epidemiological cutoff values (ECVs). From 2001 to 2009, 8,619 isolates of Candida albicans, 2,415 isolates of C. glabra...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Boyken L,Hollis RJ,Kroeger J,Messer SA,Tendolkar S,Diekema DJ

更新日期：2011-02-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:Quantitative PCR on nasopharyngeal aspirate (NPA) can achieve high sensitivity and specificity in diagnosing Pneumocystis pneumonia (PCP) compared to microscopic examination of bronchoscopic specimens in a population with low HIV prevalence. Since NPA is a minimally invasive procedure, it is ideal as a screening test ...

journal_title：Journal of clinical microbiology

authors： To KK,Wong SC,Xu T,Poon RW,Mok KY,Chan JF,Cheng VC,Chan KH,Hung IF,Yuen KY

更新日期：2013-05-01 00:00:00

abstract：:We report on a case of conjunctivitis caused by a nutritionally variant streptococcus in a newborn infant. ...

journal_title：Journal of clinical microbiology

authors： Barrios H,Bump CM

更新日期：1986-02-01 00:00:00

abstract：:The polymerase chain reaction was used to detect genomic sequences of the positive-stranded RNA of bovine viral diarrhea virus (BVDV), a member of the family Togaviridae. Using a set of 20-bp primers located within the conserved 3' region of the BVDV genome, we were able to consistently amplify a 205-bp target sequenc...

journal_title：Journal of clinical microbiology

authors： Lopez OJ,Osorio FA,Donis RO

更新日期：1991-03-01 00:00:00

abstract：:We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

journal_title：Journal of clinical microbiology

authors： Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

更新日期：1990-10-01 00:00:00

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs ...

journal_title：Journal of clinical microbiology

authors： Bass CA,Jungkind DL,Silverman NS,Bondi JM

更新日期：1993-10-01 00:00:00

abstract：:During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egy...

journal_title：Journal of clinical microbiology

authors： Kuzmin IV,Niezgoda M,Franka R,Agwanda B,Markotter W,Beagley JC,Urazova OY,Breiman RF,Rupprecht CE

更新日期：2008-04-01 00:00:00

abstract：:A 2000-2001 U.S. Haemophilus influenzae surveillance study (n = 1,434) detected nine (0.6%) beta-lactamase-negative and ampicillin-resistant (BLNAR) isolates collected from two different hospitals. The MICs of ampicillin for all nine isolates were 4 microg/ml, with results being reproducible; and all nine isolates wer...

journal_title：Journal of clinical microbiology

authors： Karlowsky JA,Critchley IA,Blosser-Middleton RS,Karginova EA,Jones ME,Thornsberry C,Sahm DF

更新日期：2002-03-01 00:00:00

abstract：:A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

journal_title：Journal of clinical microbiology

authors： Niimi K,Shepherd MG,Cannon RD

更新日期：2001-06-01 00:00:00

abstract：:Clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (NE) in poultry. This study focuses on the typing of intestinal C. perfringens isolates (n = 61) from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed mult...

journal_title：Journal of clinical microbiology

authors： Chalmers G,Bruce HL,Hunter DB,Parreira VR,Kulkarni RR,Jiang YF,Prescott JF,Boerlin P

更新日期：2008-12-01 00:00:00

abstract：:Corynebacterium group JK organisms were isolated from lung fluid aspirated with a fine needle from one of four thick-walled cavitary lesions in a 72-year-old female. Cultures and stains for mycobacteria and fungi were negative. The patient was successfully treated with an 8-week course of vancomycin. ...

journal_title：Journal of clinical microbiology

authors： McNaughton RD,Villanueva RR,Donnelly R,Freedman J,Nawrot R

更新日期：1988-10-01 00:00:00

abstract：:At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as a...

journal_title：Journal of clinical microbiology

authors： Murray L,McGowan N,Fleming J,Bailey L

更新日期：2014-10-01 00:00:00

abstract：:Ninety-eight isolates of Cryptococcus neoformans were collected from 30 patients at the University of Iowa Hospitals and Clinics from December 1987 through December 1994. The susceptibility of each isolate was determined against fluconazole, itraconazole, amphotericin B, and flucytosine. Of the 98 isolates, 53 were re...

journal_title：Journal of clinical microbiology

authors： Klepser ME,Pfaller MA

更新日期：1998-12-01 00:00:00

abstract：:A simple, sensitive, and specific polymerase chain reaction (PCR) protocol for the detection of human immunodeficiency virus type 1 (HIV-1) is described. We have improved all three PCR steps: sample preparation, DNA amplification, and detection of the amplified product. Some of the improvements have been described pre...

journal_title：Journal of clinical microbiology

authors： Albert J,Fenyö EM

更新日期：1990-07-01 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:Brain analysis cannot be used for the investigation of active lyssavirus infection in healthy bats because most bat species are protected by conservation directives. Consequently, serology remains the only tool for performing virological studies on natural bat populations; however, the presence of antibodies merely re...

journal_title：Journal of clinical microbiology

authors： Echevarría JE,Avellón A,Juste J,Vera M,Ibáñez C

更新日期：2001-10-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:Sequencing of the Mycobacterium tuberculosis pncA gene allows for pyrazinamide susceptibility testing. We summarize data on pncA polymorphisms that do not confer resistance at a susceptibility breakpoint of 100 μg/ml pyrazinamide in MGIT within a cohort of isolates from South Africa and the U.S. Centers for Disease Co...

journal_title：Journal of clinical microbiology

authors： Whitfield MG,Warren RM,Streicher EM,Sampson SL,Sirgel FA,van Helden PD,Mercante A,Willby M,Hughes K,Birkness K,Morlock G,van Rie A,Posey JE

更新日期：2015-11-01 00:00:00

abstract：:Antibodies to Japanese encephalitis virus (JEV) nonstructural 1 (NS1) protein constitute a marker of natural JEV infection among populations vaccinated with inactivated JE vaccine. In Japan, with few recent human JE cases, the natural infection rate is critical to evaluate the necessity of continuing JE vaccination. A...

journal_title：Journal of clinical microbiology

authors： Konishi E,Shoda M,Ajiro N,Kondo T

更新日期：2004-11-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

journal_title：Journal of clinical microbiology

authors： Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

更新日期：2007-08-01 00:00:00

abstract：:The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these diseases within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance of sev...

journal_title：Journal of clinical microbiology

authors： Lamagni TL,Darenberg J,Luca-Harari B,Siljander T,Efstratiou A,Henriques-Normark B,Vuopio-Varkila J,Bouvet A,Creti R,Ekelund K,Koliou M,Reinert RR,Stathi A,Strakova L,Ungureanu V,Schalén C,Strep-EURO Study Group.,Jasir A

更新日期：2008-07-01 00:00:00