Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations.

abstract：:A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

journal_title：Journal of clinical microbiology

authors： Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

更新日期：1996-06-01 00:00:00

abstract：:Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...

journal_title：Journal of clinical microbiology

authors： Kenny MT,Brackman MA

更新日期：1994-05-01 00:00:00

abstract：:The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the National Committee for Clinical Laboratory Standards (NCCLS) approved standard broth microdilution (BMD) method. The NCCLS met...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Boyken L,Messer SA,Tendolkar S,Hollis RJ

更新日期：2003-05-01 00:00:00

abstract：:The accessory gene regulator (agr) is a central system that controls the expression of Staphylococcus aureus virulence factors. In this study, the distribution of agr alleles, defined by agr restriction fragment length polymorphism within agr interference groups, among S. aureus isolates from bovine and human origin w...

journal_title：Journal of clinical microbiology

authors： Gilot P,van Leeuwen W

更新日期：2004-03-01 00:00:00

abstract：:Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six ot...

journal_title：Journal of clinical microbiology

authors： Miyakawa Y,Kagaya K,Fukazawa Y,Soe G

更新日期：1986-05-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

journal_title：Journal of clinical microbiology

authors： Townsend EL,Maheswaran SK,Leininger JR,Ames TR

更新日期：1987-02-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:An enzyme-linked immunosorbent microassay using nitrocellulose paper as the solid-phase support was developed for the detection of peste-des-petits-ruminants virus antigens in infected caprine tissue homogenates. Dots of tissue homogenates were applied to nitrocellulose papers, and any unreacted sites were blocked wit...

journal_title：Journal of clinical microbiology

authors： Obi TU,Ojeh CK

更新日期：1989-09-01 00:00:00

abstract：:The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in ...

journal_title：Journal of clinical microbiology

authors： Knox CM,Cevellos V,Dean D

更新日期：1998-12-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:We investigated whether methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates with low-level mupirocin resistance can serve as recipients of a pSK41-like plasmid conferring high-level mupirocin resistance without substantial fitness cost. Our results suggest that acquisition of the plasmid conferrin...

journal_title：Journal of clinical microbiology

authors： Mongkolrattanothai K,Pumfrey L,Mankin P,Gray BM

更新日期：2009-12-01 00:00:00

abstract：:Reference strains of serotypes 1 to 12 of Actinobacillus (Haemophilus) pleuropneumoniae were cultured in Eagle minimal essential medium with 10% Serum Plus. Culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. All strains except the reference s...

journal_title：Journal of clinical microbiology

authors： Kamp EM,van Leengoed LA

更新日期：1989-06-01 00:00:00

abstract：:The dimorphic fungus, Penicillium marneffei, is an emerging opportunistic pathogen endemic in Southeast Asia, especially for those with impaired cellular immunity such as human immunodeficiency virus-infected persons. A discriminatory and reproducible method based on the analysis of nucleotide sequences would facilita...

journal_title：Journal of clinical microbiology

authors： Lasker BA

更新日期：2006-09-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Grewal HM,Gaastra W,Svennerholm AM,Bhan MK

更新日期：1992-07-01 00:00:00

abstract：:The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase ...

journal_title：Journal of clinical microbiology

authors： Rüsch-Gerdes S,Pfyffer GE,Casal M,Chadwick M,Siddiqi S

更新日期：2006-03-01 00:00:00

abstract：:An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in s...

journal_title：Journal of clinical microbiology

authors： Roosendaal R,Vos JH,Roumen T,van Vugt R,Cattoli G,Bart A,Klaasen HL,Kuipers EJ,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2000-07-01 00:00:00

abstract：:The elimination of leprosy continues to be a challenge, with the disease remaining endemic in several countries. India accounts for the highest number of cases, and the identification of child cases indicates recent transmission. Genetic markers, like variable-number tandem repeats (VNTRs) and single-nucleotide polymo...

journal_title：Journal of clinical microbiology

authors： Kuruwa S,Vissa V,Mistry N

更新日期：2012-04-01 00:00:00

abstract：:An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...

journal_title：Journal of clinical microbiology

authors： Faulde M,Putzker M,Mertes T,Sobe D

更新日期：1991-02-01 00:00:00

abstract：:Many diseases with unknown etiology may be caused by unidentified viruses. Sequence-independent amplification revealed a new astrovirus, similar to VA1, in a 4-year-old male diagnosed with celiac disease. This expands the geographic range of this virus to include Europe and may associate astrovirus infection with the ...

journal_title：Journal of clinical microbiology

authors： Smits SL,van Leeuwen M,van der Eijk AA,Fraaij PL,Escher JC,Simon JH,Osterhaus AD

更新日期：2010-09-01 00:00:00

abstract：:The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...

journal_title：Journal of clinical microbiology

authors： Saribasak H,Salih BA,Yamaoka Y,Sander E

更新日期：2004-04-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

journal_title：Journal of clinical microbiology

authors： Heck FC,Williams JD,Pruett J

更新日期：1980-04-01 00:00:00

abstract：:The cellular fatty acid composition of Legionella feeleii was determined by capillary gas-liquid chromatography, and the ubiquinone content was determined by reverse-phase high-pressure liquid chromatography. As in other Legionella species, this new species is characterized by relatively large amounts of branched-chai...

journal_title：Journal of clinical microbiology

authors： Moss CW,Bibb WF,Karr DE,Guerrant GO,Lambert MA

更新日期：1983-10-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus d...

journal_title：Journal of clinical microbiology

authors： Tremblay CL,Giguel F,Merrill DP,Wong JT,Rosenberg E,Kalams S,Walker BD,D'Aquila RT,Hirsch MS

更新日期：2000-11-01 00:00:00

abstract：:This study aimed to assess the interlaboratory reproducibility at four university hospital laboratories in the southeast region of France of the Etest technique for the determination of caspofungin (CAS) and amphotericin B (AMB) MICs and to compare it to the CLSI broth microdilution reference method. Consecutive clini...

journal_title：Journal of clinical microbiology

authors： Ranque S,Lachaud L,Gari-Toussaint M,Michel-Nguyen A,Mallié M,Gaudart J,Bertout S

更新日期：2012-07-01 00:00:00

abstract：:We previously demonstrated the transmission of murine cytomegalovirus to syngeneic mice and preformed monolayers of mouse embryo fibroblasts with plastic-adherent peritoneal exudate cells from infected mice as a source of macrophages. In the present studies we compared this standard feeder layer method with a reverse ...

journal_title：Journal of clinical microbiology

authors： Winkler SR,Booss J

更新日期：1980-12-01 00:00:00

abstract：:Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern an...

journal_title：Journal of clinical microbiology

authors： Migheli Q,Balmas V,Harak H,Sanna S,Scherm B,Aoki T,O'Donnell K

更新日期：2010-04-01 00:00:00

abstract：:The human erythrocyte receptor which mediates mannose-resistant hemagglutination by enterotoxigenic Escherichia coli possessing colonization factor antigen II is not universally distributed among donors. Mannose-resistant hemagglutination-positive erythrocytes are more common among black donors than nonblack donors; t...

journal_title：Journal of clinical microbiology

authors： Evans DJ Jr,Evans DG,Diaz SR,Graham DY

更新日期：1988-09-01 00:00:00