Detection of immunoglobulin G to Pasteurella haemolytica capsular polysaccharide by enzyme-linked immunosorbent assay.

abstract：:Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew Clostridium argentinense. This case highlights the...

journal_title：Journal of clinical microbiology

authors： Taylor SM,Wolfe CR,Dixon TC,Ruch DS,Cox GM

更新日期：2010-02-01 00:00:00

abstract：:In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

journal_title：Journal of clinical microbiology

authors： Beall B,Gherardi G,Facklam RR,Hollingshead SK

更新日期：2000-10-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:Dogs orally infected with Neorickettsia helminthoeca developed immunoglobulin G titers against Erlichia risticii, Erlichia sennetsu, and Erlichia canis similar to those against N. helminthoeca antigen, as determined by immunofluorescence. Western immunoblotting showed that the major common antigens shared among the mi...

journal_title：Journal of clinical microbiology

authors： Rikihisa Y

更新日期：1991-09-01 00:00:00

abstract：:Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

journal_title：Journal of clinical microbiology

authors： Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

更新日期：2015-07-01 00:00:00

abstract：:More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five peer-group laboratories. The comparison indicates that the automated system recognizes the nine groups of significant mic...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Gavan TL,Sonnenwirth A,Taylor WI,Washington JA 2nd

更新日期：1979-08-01 00:00:00

abstract：:Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...

journal_title：Journal of clinical microbiology

authors： Reijans M,Dingemans G,Klaassen CH,Meis JF,Keijdener J,Mulders B,Eadie K,van Leeuwen W,van Belkum A,Horrevorts AM,Simons G

更新日期：2008-04-01 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

更新日期：1995-06-01 00:00:00

abstract：:The Limulus amoebocyte lysate (LAL) test was evaluated for rapid detection of gram-negative bacteriuria in an adult patient population. Time to gelation of a standard LAL preparation was used as a measure of significant (greater than 10(5) bacteria per ml) gram-negative bacteriuria, and the results of 190 LAL assays w...

journal_title：Journal of clinical microbiology

authors： Nachum R,Shanbrom E

更新日期：1981-01-01 00:00:00

abstract：:A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h. ...

journal_title：Journal of clinical microbiology

authors： Clemons KV,Gadberry JL

更新日期：1982-04-01 00:00:00

abstract：:The Enzygnost alpha method was tested against the complement fixation test and anti-VCA immunofluorescence to determine the respective titers of anti-cytomegalovirus and anti-Epstein-Barr virus immunoglobulin G antibodies. For cytomegalovirus, the Enzygnost results showed 97.99% agreement with the readings obtained by...

journal_title：Journal of clinical microbiology

authors： Gutierrez J,Maroto MD,Piédrola G

更新日期：1994-10-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:The spores of Henneguya salminicola, a common tissue parasite of salmonid fishes in the northern hemisphere, were observed in stool specimens from two different patients with diarrhea. The spores' superficial resemblance to human spermatozoa resulted, in one instance, in an incorrect report, leading to suspicion of se...

journal_title：Journal of clinical microbiology

authors： McClelland RS,Murphy DM,Cone DK

更新日期：1997-11-01 00:00:00

abstract：:The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

journal_title：Journal of clinical microbiology

authors： Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

更新日期：2003-04-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:The diagnosis of filarial infections among individuals residing in areas where the disease is not endemic requires both strong clinical suspicion and expert training in infrequently practiced parasitological methods. Recently developed filarial molecular diagnostic assays are highly sensitive and specific but have lim...

journal_title：Journal of clinical microbiology

authors： Fink DL,Fahle GA,Fischer S,Fedorko DF,Nutman TB

更新日期：2011-01-01 00:00:00

abstract：:The use of 1% unmodified rice starch and 1% horse serum instead of 2% soluble starch and 5% serum in Granada medium is described. These components result in a medium of increased stability, preventing spoilage after a few days of storage at room temperature. ...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodríguez-Granger J,Camacho-Muñoz E,Sampedro A

更新日期：2005-04-01 00:00:00

abstract：:Transmissible spongiform encephalopathy strains can be differentiated by their behavior in bioassays and by molecular analyses of the disease-associated prion protein (PrP) in a posttranslationally transformed conformation (PrPSc). Until recently, isolates from cases of bovine spongiform encephalopathy (BSE) appeared ...

journal_title：Journal of clinical microbiology

authors： Jacobs JG,Langeveld JP,Biacabe AG,Acutis PL,Polak MP,Gavier-Widen D,Buschmann A,Caramelli M,Casalone C,Mazza M,Groschup M,Erkens JH,Davidse A,van Zijderveld FG,Baron T

更新日期：2007-06-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00

abstract：:Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-speci...

journal_title：Journal of clinical microbiology

authors： Kim CM,Song ES,Jang HJ,Kim HJ,Lee S,Shin JH,Kim SJ,Jeong SH,Jeong J,Koh K,Choi GE,Lee EY,Chang CL

更新日期：2010-05-01 00:00:00

abstract：:Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

journal_title：Journal of clinical microbiology

authors： Shibl AM,Al-Sowaygh IA

更新日期：1980-02-01 00:00:00

abstract：:The serum antibody response of infants and children immunized with Formalin-inactivated respiratory syncytial virus (RSV) vaccine 20 years ago was determined by using an enzyme-linked immunosorbent assay specific for the RSV fusion (F) and large (G) glycoproteins and a neutralization assay. Twenty-one young infants (2...

journal_title：Journal of clinical microbiology

authors： Murphy BR,Prince GA,Walsh EE,Kim HW,Parrott RH,Hemming VG,Rodriguez WJ,Chanock RM

更新日期：1986-08-01 00:00:00

abstract：:Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for ...

journal_title：Journal of clinical microbiology

authors： Merlino J,Gill R,Robertson GJ

更新日期：1996-12-01 00:00:00

abstract：:Pulsed-field gel electrophoresis revealed that multiple consecutive isolates of Legionella pneumophila from two cardiac transplant patients remained genomically stable, despite exposure to host defenses and antimicrobial agents. ...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Johnson WM,Tyler SD,Bezanson GS,Burbridge S

更新日期：1994-12-01 00:00:00

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:The use of rapid tests for human immunodeficiency virus (HIV) has become standard in HIV testing algorithms employed in resource-limited settings. We report an extensive HIV rapid test validation study conducted among Ugandan blood bank donors at low risk for HIV infection. The operational characteristics of four read...

journal_title：Journal of clinical microbiology

authors： Eller LA,Eller MA,Ouma BJ,Kataaha P,Bagaya BS,Olemukan RL,Erima S,Kawala L,de Souza MS,Kibuuka H,Wabwire-Mangen F,Peel SA,O'Connell RJ,Robb ML,Michael NL

更新日期：2007-10-01 00:00:00

abstract：:Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

journal_title：Journal of clinical microbiology

authors： Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

更新日期：1996-08-01 00:00:00

abstract：:The beta and gamma genera of papillomaviruses consist of epidermodysplasia verruciformis-related human papillomaviruses (HPVs) and phylogenetically related cutaneous HPVs. Here, we have developed a consensus primer PCR assay and reverse line blot typing system coupled thereto (referred to as beta and gamma cutaneous H...

journal_title：Journal of clinical microbiology

authors： Brink AA,Lloveras B,Nindl I,Heideman DA,Kramer D,Pol R,Fuente MJ,Meijer CJ,Snijders PJ

更新日期：2005-11-01 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00