Evaluation of a new reagent for anti-cytomegalovirus and anti-Epstein-Barr virus immunoglobulin G.

abstract：:In order to better understand the role of Mycobacterium leprae nasal carriage in the maintenance of infection reservoirs and transmission of leprosy, we applied a polymerase chain reaction (PCR) that detected a 531-bp fragment of the pra gene of M. leprae on nasal swab specimens collected through a total population su...

journal_title：Journal of clinical microbiology

authors： Klatser PR,van Beers S,Madjid B,Day R,de Wit MY

更新日期：1993-11-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacter jejuni (16 strains) from Campylobacter coli (10 strains). This observation was confirmed by the study of DNA homology obtained by a quantitative filter hybridization method. The hippurate hydrolysis tes...

journal_title：Journal of clinical microbiology

authors： Derclaye I,Delor I,Van Bouchaute M,Moureau P,Wauters G,Cornelis GR

更新日期：1989-05-01 00:00:00

abstract：:Bacterial vaginosis (BV) is the most common cause of vaginal discharge in reproductive-age women. BV has been associated with poor reproductive outcomes such as preterm delivery, the acquisition of sexually transmitted infections, including HIV, and pelvic inflammatory disease. BV represents the acquisition of a diver...

journal_title：Journal of clinical microbiology

authors： Coleman JS,Gaydos CA

更新日期：2018-08-27 00:00:00

abstract：:Failure to demonstrate immunoglobulin M (IgM) antibodies by indirect immunofluorescence (IgM-IFA) in sera from some patients with acute acquired toxoplasmosis has recently been attributed to an inhibitory effect of high titers of IgG antibodies in these sera (Pyndiah et al. J. Clin. Microbiol. 9:170-174, 1979). To con...

journal_title：Journal of clinical microbiology

authors： Filice GA,Yeager AS,Remington JS

更新日期：1980-09-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. A...

journal_title：Journal of clinical microbiology

authors： Hassan AA,Khan IU,Abdulmawjood A,Lämmler C

更新日期：2001-04-01 00:00:00

abstract：:This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

journal_title：Journal of clinical microbiology

authors： Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

更新日期：2014-12-01 00:00:00

abstract：:Iodophor and alcohol pledgets were compared with the Medi-Flex Prep Kit II for skin disinfection before venipuncture. Of 12,367 blood cultures collected, 6,362 were done with conventional pledgets and 6, 005 were done with Medi-Flex kits. Contamination occurred in 351 of 6,362 blood cultures (5.5%; range, 3.7 to 8.1%)...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Mirrett S,Reimer LG,Fernando C,Meredith FT,Reller LB

更新日期：2000-12-01 00:00:00

abstract：:We examined Enterococcus faecalis strains clinically isolated from 100 patients with urinary tract infections (UTIs) for their susceptibility to levofloxacin (LVX) by measuring the MIC and investigated amino acid mutations by direct DNA sequencing, which were then correlated with LVX resistance. Next, we studied risk ...

journal_title：Journal of clinical microbiology

authors： Yasufuku T,Shigemura K,Shirakawa T,Matsumoto M,Nakano Y,Tanaka K,Arakawa S,Kawabata M,Fujisawa M

更新日期：2011-11-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as indicated by the abse...

journal_title：Journal of clinical microbiology

authors： Schoeb TR,Dybvig K,Keisling KF,Davidson MK,Davis JK

更新日期：1997-07-01 00:00:00

abstract：:Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gen...

journal_title：Journal of clinical microbiology

authors： Nethercott C,Mabbett AN,Totsika M,Peters P,Ortiz JC,Nimmo GR,Coombs GW,Walker MJ,Schembri MA

更新日期：2013-07-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:Of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (Ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (Ad1 in two cases, Ad18 or Ad31) which was selected over Ad40 during serial passage in the 293 cells. The selection of Ad1 over Ad40...

journal_title：Journal of clinical microbiology

authors： Brown M

更新日期：1985-08-01 00:00:00

abstract：:Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping have focused ...

journal_title：Journal of clinical microbiology

authors： Batt SL,Charalambous BM,McHugh TD,Martin S,Gillespie SH

更新日期：2005-06-01 00:00:00

abstract：:Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in t...

journal_title：Journal of clinical microbiology

authors： Li H,Dyer N,Keller J,Crawford TB

更新日期：2000-04-01 00:00:00

abstract：:Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping signatures may enable the analysis of the strain population structure in different settings and will enable the rapid identification o...

journal_title：Journal of clinical microbiology

authors： Streicher EM,Victor TC,van der Spuy G,Sola C,Rastogi N,van Helden PD,Warren RM

更新日期：2007-01-01 00:00:00

abstract：:Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

journal_title：Journal of clinical microbiology

authors： Truant AL,Barksdale BL,Huber TW,Elliott LB

更新日期：1983-01-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:This study evaluated the performance of the Rapidec Carba NP test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing Enterobacteriaceae clinical isolates. In total, 252 clinical Enterobacteriaceae isolates that had been genetically ...

journal_title：Journal of clinical microbiology

authors： Hombach M,von Gunten B,Castelberg C,Bloemberg GV

更新日期：2015-12-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:Three variants of a Corynebacterium diphtheriae subsp. mitis strain belfanti, two toxigenic (phage types 7 and 11) and one nontoxigenic (phage type 7), were simultaneously isolated from the throat of an adult. ...

journal_title：Journal of clinical microbiology

authors： Chang DN,Laughren GS,Chalvardjian NE

更新日期：1978-12-01 00:00:00

abstract：:Aspergillus terreus isolates collected from patients at The M. D. Anderson Cancer Center in Houston, TX, and at The University Hospital of Innsbruck, Austria, were analyzed using random amplification of polymorphic DNA-PCR with three different primers. No strain similarity in either institution was detected, indicatin...

journal_title：Journal of clinical microbiology

authors： Lass-Flörl C,Grif K,Kontoyiannis DP

更新日期：2007-08-01 00:00:00

abstract：:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, de...

journal_title：Journal of clinical microbiology

authors： Luker G,Chow C,Richards DF,Johnson FB

更新日期：1991-07-01 00:00:00

abstract：:Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. R...

journal_title：Journal of clinical microbiology

authors： Proenca-Modena JL,Paula FE,Buzatto GP,Carenzi LR,Saturno TH,Prates MC,Silva ML,Delcaro LS,Valera FC,Tamashiro E,Anselmo-Lima WT,Arruda E

更新日期：2014-08-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal. ...

journal_title：Journal of clinical microbiology

authors： Johnston SP,Pieniazek NJ,Xayavong MV,Slemenda SB,Wilkins PP,da Silva AJ

更新日期：2006-03-01 00:00:00

abstract：:A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a ...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Helden PD

更新日期：1992-06-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00