PCR as a confirmatory technique for laboratory diagnosis of malaria.

Abstract:

:We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.

journal_name

J Clin Microbiol

authors

Johnston SP,Pieniazek NJ,Xayavong MV,Slemenda SB,Wilkins PP,da Silva AJ

doi

10.1128/JCM.44.3.1087-1089.2006

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

1087-9

issue

3

eissn

0095-1137

issn

1098-660X

pii

44/3/1087

journal_volume

44

pub_type

杂志文章
  • Porphyromonas gingivalis strain diversity.

    abstract::Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Genotyping studies suggest that genetic variability exists among P. gingivalis strains; however, the extent of variability remains unclear and regions of variability remain largely unidentified. To assess P. gingivalis strain diversity, w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00569-09

    authors: Igboin CO,Griffen AL,Leys EJ

    更新日期:2009-10-01 00:00:00

  • Detection of cytomegalovirus from clinical specimens in centrifugation culture by in situ DNA hybridization and monoclonal antibody staining.

    abstract::An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.1.21-23.1989

    authors: Gleaves CA,Hursh DA,Rice DH,Meyers JD

    更新日期:1989-01-01 00:00:00

  • Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.

    abstract::In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4486-4492.2002

    authors: Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

    更新日期:2002-12-01 00:00:00

  • Allele-specific real-time PCR system for detection of subpopulations of genotype 1a and 1b hepatitis C NS5B Y448H mutant viruses in clinical samples.

    abstract::The Y448H mutation in NS5B has been selected by GS-9190 as well as several benzothiadiazine hepatitis C virus (HCV) polymerase inhibitors in vitro and in vivo. However, the level and the evolution kinetics of this resistance mutation prior to and during treatment are poorly understood. In this study, we developed an a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00274-11

    authors: Bae AS,Ku KS,Miller MD,Mo H,Svarovskaia ES

    更新日期:2011-09-01 00:00:00

  • Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene.

    abstract::To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.7.2756-2759.2000

    authors: Pietilä J,He Q,Oksi J,Viljanen MK

    更新日期:2000-07-01 00:00:00

  • Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum.

    abstract::The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.5.970-974.1990

    authors: Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

    更新日期:1990-05-01 00:00:00

  • Persistence of Escherichia coli O157 isolates on bovine farms in England and Wales.

    abstract::We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 mo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.2.898-902.2005

    authors: Liebana E,Smith RP,Batchelor M,McLaren I,Cassar C,Clifton-Hadley FA,Paiba GA

    更新日期:2005-02-01 00:00:00

  • Use of DNA analysis of Pasteurella haemolytica biotype T isolates to monitor transmission in bighorn sheep (Ovis canadensis canadensis).

    abstract::Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in central Idaho. Pasteurella haemolytica was isolated from five adult Rocky Mountain bighorn ewes captured from a free-ranging herd in central Idaho. The lambs from two...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.4.831-835.1993

    authors: Jaworski MD,Ward AC,Hunter DL,Wesley IV

    更新日期:1993-04-01 00:00:00

  • Molecular epidemiology of an outbreak caused by methicillin-resistant Staphylococcus aureus in a health care ward and associated nursing home.

    abstract::Our point-prevalence survey followed an outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in a long-term care facility and identified five MRSA strains, of which two possessed an outbreak genotype not encountered previously and three had another profile. All of them possessed SCCmec type V. Six methicilli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.12.6161-6163.2005

    authors: Kerttula AM,Lyytikäinen O,Vuopio-Varkila J,Ibrahem S,Agthe N,Broas M,Jägerroos H,Virolainen A

    更新日期:2005-12-01 00:00:00

  • Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D).

    abstract::Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.8.2118-2123.1993

    authors: Luk JM,Kongmuang U,Reeves PR,Lindberg AA

    更新日期:1993-08-01 00:00:00

  • Microbiological Characteristics of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in South African Women.

    abstract::We analyzed data of 263 women with at least one genital or anorectal sexually transmitted infection from a cross-sectional study conducted in rural South Africa. We provide new insights concerning the concurrence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis infectio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02848-15

    authors: Dubbink JH,de Waaij DJ,Bos M,van der Eem L,Bébéar C,Mbambazela N,Ouburg S,Peters RP,Morré SA

    更新日期:2016-01-01 00:00:00

  • First human systemic infection caused by Spiroplasma.

    abstract::Spiroplasma species are organisms that normally colonize plants and insects. We describe the first case of human systemic infection caused by Spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. Spiroplasma turonicum was identified th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02841-14

    authors: Aquilino A,Masiá M,López P,Galiana AJ,Tovar J,Andrés M,Gutiérrez F

    更新日期:2015-02-01 00:00:00

  • Evaluation of a prereduced anaerobically sterilized medium (PRAS II) system for identification anaerobic microorganisms.

    abstract::A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.3.570-572.1982

    authors: Beaucage CM,Onderdonk AB

    更新日期:1982-09-01 00:00:00

  • Molecular epidemiology of clinical isolates of methicillin-resistant Staphylococcus aureus in Taiwan.

    abstract::During July 2000 and October 2001, a total of 595 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were collected from six medical centers distributed in northern, central, and southern Taiwan. Specimen sources included blood (n = 279), pus (n = 173), sputum (n = 94), body fluids (n = 21), cathe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.1.307-310.2004

    authors: Huang YC,Su LH,Wu TL,Liu CE,Young TG,Chen PY,Hseuh PR,Lin TY

    更新日期:2004-01-01 00:00:00

  • Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species.

    abstract::A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.3.513-518.1981

    authors: Shinoda T,Kaufman L,Padhye AA

    更新日期:1981-03-01 00:00:00

  • Biochemical characterization of Haemophilus species with the minitek differentiation system.

    abstract::Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.14.5.579-581.1981

    authors: Kawakami Y,Okimura Y,Kanai M

    更新日期:1981-11-01 00:00:00

  • Evaluation of three influenza A and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses.

    abstract::The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 30 influenza A/H3 vi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02464-09

    authors: Selvaraju SB,Selvarangan R

    更新日期:2010-11-01 00:00:00

  • Vancomycin-resistant enterococci colonizing the intestinal tracts of hospitalized patients.

    abstract::A point prevalence culture survey was carried out to investigate the prevalence of fecal carriage of vancomycin-resistant enterococci (VRE) among patients admitted to an 800-bed general hospital where no VRE had been isolated previously. Twenty-two of 636 patients (3.5%) were found to be VRE carriers. Eighteen strains...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.11.2842-2846.1995

    authors: Gordts B,Van Landuyt H,Ieven M,Vandamme P,Goossens H

    更新日期:1995-11-01 00:00:00

  • Clinical significance of mites in urine.

    abstract::We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.12.6200-6201.2005

    authors: Dini LA,Frean JA

    更新日期:2005-12-01 00:00:00

  • Immunodiagnosis of Ehrlichia canis infection with recombinant proteins.

    abstract::Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.1.315-322.2001

    authors: McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

    更新日期:2001-01-01 00:00:00

  • Clinical value of specific immunoglobulin E detection by enzyme-linked immunosorbent assay in cases of acquired and congenital toxoplasmosis.

    abstract::The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.4.1681-1686.2003

    authors: Foudrinier F,Villena I,Jaussaud R,Aubert D,Chemla C,Martinot F,Pinon JM

    更新日期:2003-04-01 00:00:00

  • Typing Candida albicans oral isolates from human immunodeficiency virus-infected patients by multilocus enzyme electrophoresis and DNA fingerprinting.

    abstract::A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.5.1235-1248.1996

    authors: Boerlin P,Boerlin-Petzold F,Goudet J,Durussel C,Pagani JL,Chave JP,Bille J

    更新日期:1996-05-01 00:00:00

  • Characterization of Borrelia burgdorferi isolated from erythema migrans lesions: interrelationship of three molecular typing methods.

    abstract::Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.8.2954-2957.2001

    authors: Iyer R,Liveris D,Adams A,Nowakowski J,McKenna D,Bittker S,Cooper D,Wormser GP,Schwartz I

    更新日期:2001-08-01 00:00:00

  • Characterization of clinically significant strains of coagulase-negative staphylococci.

    abstract::On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.2.258-269.1983

    authors: Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

    更新日期:1983-08-01 00:00:00

  • Recovery of Blastomyces dermatitidis from blood of a patient with disseminated blastomycosis.

    abstract::This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.8.1421-1423.1987

    authors: Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

    更新日期:1987-08-01 00:00:00

  • Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods.

    abstract::Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.5.2449-2451.2005

    authors: Tsang RS,Sill ML,Advani A,Xing D,Newland P,Hallander H

    更新日期:2005-05-01 00:00:00

  • Armored RNA as virus surrogate in a real-time reverse transcriptase PCR assay proficiency panel.

    abstract::In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.1.67-70.2006

    authors: Hietala SK,Crossley BM

    更新日期:2006-01-01 00:00:00

  • Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications.

    abstract::As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02161-20

    authors: Rodrigues CMC,Jolley KA,Smith A,Cameron JC,Feavers IM,Maiden MCJ

    更新日期:2020-12-17 00:00:00

  • Evaluation of GeneXpert MTB/RIF for diagnosis of tuberculous meningitis.

    abstract::Tuberculous meningitis (TBM) is the most severe form of tuberculosis. Microbiological confirmation is rare, and treatment is often delayed, increasing mortality and morbidity. The GeneXpert MTB/RIF test was evaluated in a large cohort of patients with suspected tuberculous meningitis. Three hundred seventy-nine patien...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01834-13

    authors: Nhu NT,Heemskerk D,Thu do DA,Chau TT,Mai NT,Nghia HD,Loc PP,Ha DT,Merson L,Thinh TT,Day J,Chau Nv,Wolbers M,Farrar J,Caws M

    更新日期:2014-01-01 00:00:00

  • Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to ospA genotype and evidence of lateral gene exchange of ospC.

    abstract::It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.7.1860-1866.1995

    authors: Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

    更新日期:1995-07-01 00:00:00