Suitability of infection of cells in suspension for detection of herpes simplex virus.

abstract：:We report on a case of conjunctivitis caused by a nutritionally variant streptococcus in a newborn infant. ...

journal_title：Journal of clinical microbiology

authors： Barrios H,Bump CM

更新日期：1986-02-01 00:00:00

abstract：:Although rotavirus genotype P[6] is one of the three most common VP4 specificities associated with human infection, the relatively few sequence data available in public databases suggest that the genetic variability within P[6] might be presently unexplored. Thus far, two human P[6] lineages (M37-like and AU19-like) a...

journal_title：Journal of clinical microbiology

authors： Bányai K,Martella V,Jakab F,Melegh B,Szücs G

更新日期：2004-09-01 00:00:00

abstract：:We evaluated a commercially available second-generation anti-H. pylori immunoglobulin G enzyme immunoassay (EIA) (Cobas Core Anti-Helicobacter pylori EIA; Roche S. A., Basel, Switzerland) for serodiagnosis of H. pylori infection. The results of the assay were assessed in relation to the results of bacterial culture, u...

journal_title：Journal of clinical microbiology

authors： Goossens H,Glupczynski Y,Burette A,Van den Borre C,Butzler JP

更新日期：1992-01-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream isolates were tested to determine the prevalence of vancomycin heteroresistance. We screened 619 ORSA nosocomial bloodstream isolates from 36 hospitals between 1997 and 2000. Only one isolate exhibiting heterotypic resistance was detected. Thus, v...

journal_title：Journal of clinical microbiology

authors： Tallent SM,Bischoff T,Climo M,Ostrowsky B,Wenzel RP,Edmond MB

更新日期：2002-06-01 00:00:00

abstract：:By serological methods, all 83 known types of Streptococcus pneumoniae could be shown to possess C polysaccharide and F antigen. Cross-reactions due to these two antigens between pneumococci and a broad range of most other commonly encountered streptococci were examined. The presence of an antigen closely similar or i...

journal_title：Journal of clinical microbiology

authors： Sørensen UB,Henrichsen J

更新日期：1987-10-01 00:00:00

abstract：:In this study we evaluated the ability of three commercial methods, API 20A (Analytab Products, Plainview, N.Y.), Minitek (BBL Microbiology Systems, Cockeysville, Md.), and Anaerobe-Tek (Flow Laboratories, Inc., McLean, Va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria withou...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Kaufmann CS,Keifer JC,Venbrux HJ

更新日期：1983-09-01 00:00:00

abstract：:The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase ...

journal_title：Journal of clinical microbiology

authors： Rüsch-Gerdes S,Pfyffer GE,Casal M,Chadwick M,Siddiqi S

更新日期：2006-03-01 00:00:00

abstract：:Twelve strains of Pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the People's Republic of China were compared with serogroups in the International Antigenic Typing System (IATS). The first eight groups originated in the People's Republic of China, and group II appears to have a ne...

journal_title：Journal of clinical microbiology

authors： Liu PV

更新日期：1987-05-01 00:00:00

abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...

journal_title：Journal of clinical microbiology

authors： Kurzai O,Heinz WJ,Sullivan DJ,Coleman DC,Frosch M,Mühlschlegel FA

更新日期：1999-05-01 00:00:00

abstract：:We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

journal_title：Journal of clinical microbiology

authors： Nace EK,Steurer FJ,Eberhard ML

更新日期：1999-12-01 00:00:00

abstract：:An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for...

journal_title：Journal of clinical microbiology

authors： Bardell D

更新日期：1975-02-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:The method of choice for the detection of Lassa virus is reverse transcription (RT)-PCR. However, the high degree of genetic variability of the virus poses a problem with the design of RT-PCR assays that will reliably detect all strains. Recently, we encountered difficulties in detecting some strains from Liberia and ...

journal_title：Journal of clinical microbiology

authors： Olschläger S,Lelke M,Emmerich P,Panning M,Drosten C,Hass M,Asogun D,Ehichioya D,Omilabu S,Günther S

更新日期：2010-06-01 00:00:00

abstract：:Salmonella enterica subsp. enterica serovar Agona was not frequently encountered in Finland until an increase in rates of isolation among animal and feed was seen in 1994. A small outbreak among cattle farms in the regions of Oulu and Vaasa in northwestern Finland in 1994-1995 included eight farms. After the outbreak,...

journal_title：Journal of clinical microbiology

authors： Lindqvist N,Siitonen A,Pelkonen S

更新日期：2002-10-01 00:00:00

abstract：:A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiolog...

journal_title：Journal of clinical microbiology

authors： Cacho JB,Aguirre PM,Hernanz A,Velasco AC

更新日期：1989-02-01 00:00:00

abstract：:Probable transmission of an extended-spectrum-beta-lactamase-producing Escherichia coli strain (sequence type ST131) between a father and daughter was documented. The father developed severe, recurrent pyelonephritis with multiple small abscesses; the daughter later developed septic shock, bacteremia, and extensive em...

journal_title：Journal of clinical microbiology

authors： Ender PT,Gajanana D,Johnston B,Clabots C,Tamarkin FJ,Johnson JR

更新日期：2009-11-01 00:00:00

abstract：:A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% ...

journal_title：Journal of clinical microbiology

authors： Vinayak VK,Purnima,Singh K,Venkatwswarlu K,Nain CK,Mehta SK

更新日期：1986-06-01 00:00:00

abstract：:Prostate tissues from patients with prostate cancer and benign prostatic hyperplasia (BPH) frequently contain histological inflammation, and a proportion of these patients show evidence of Propionibacterium acnes infection in the prostate gland. We developed a multicolor fluorescent in situ hybridization (FISH) assay ...

journal_title：Journal of clinical microbiology

authors： Alexeyev OA,Marklund I,Shannon B,Golovleva I,Olsson J,Andersson C,Eriksson I,Cohen R,Elgh F

更新日期：2007-11-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:We report a fatal case of acute lower respiratory tract disease with human rhinovirus C (HRV-C) as the unique cause in a 19-month-old girl with a history of repeated episodes of bronchiolitis. HRV-C type 8 nucleic acids were observed in respiratory, stool, and cerebrospinal fluid samples, and infectious virions were i...

journal_title：Journal of clinical microbiology

authors： Lupo J,Schuffenecker I,Morel-Baccard C,Bardet J,Payen V,Kaiser L,Constant S,Lobrinus JA,Lin-Marq N,Lina B,Morand P,Tapparel C

更新日期：2015-05-01 00:00:00

abstract：:Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...

journal_title：Journal of clinical microbiology

authors： Aoki FH,Imai T,Tanaka R,Mikami Y,Taguchi H,Nishimura NF,Nishimura K,Miyaji M,Schreiber AZ,Branchini ML

更新日期：1999-02-01 00:00:00

abstract：:We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs wa...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-11-01 00:00:00

abstract：:The prevalence of coinfection of Campylobacter species in dogs was determined using four isolation methods. In 26% of the positive-testing stools, multiple Campylobacter species were identified. The use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection ...

journal_title：Journal of clinical microbiology

authors： Koene MG,Houwers DJ,Dijkstra JR,Duim B,Wagenaar JA

更新日期：2004-02-01 00:00:00

abstract：:Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

journal_title：Journal of clinical microbiology

authors： Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

更新日期：1998-06-01 00:00:00

abstract：:The detection of the human RNA viruses, calicivirus and astrovirus, requires high sensitivity and broad reactivity. A novel single-tube nested reverse transcription-PCR (RT-PCR) method is described here, in which all of the required reagents are included in the one tube; however, those required for the nested amplific...

journal_title：Journal of clinical microbiology

authors： Ratcliff RM,Doherty JC,Higgins GD

更新日期：2002-11-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Tiu FS,Stamm WE

更新日期：1995-09-01 00:00:00

abstract：:A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR t...

journal_title：Journal of clinical microbiology

authors： Cleary TJ,Roudel G,Casillas O,Miller N

更新日期：2003-10-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for blastomycosis was evaluated with 65 human sera from culturally or histologically proven cases of blastomycosis, 53 sera from humans with heterologous infections, and 115 sera from apparently normal people. The diagnostic value of the ELISA was compared with that of the ...

journal_title：Journal of clinical microbiology

authors： Turner S,Kaufman L,Jalbert M

更新日期：1986-02-01 00:00:00