Suitability of infection of cells in suspension for detection of herpes simplex virus.


:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, depending on the cell line used in the tests. The mean time to diagnosis by the suspension-infection technique was 1 day, compared with 4.8 days by conventional culture. Comparable detection of low-level positive specimens was observed with the methods. In a clinical setting, the isolation rates obtained by suspension-infection and conventional culture were indistinguishable. These results indicate that the suspension-infection method can be used for the detection of herpes simplex virus and can yield rapid diagnostic results without a time-consuming centrifugation step.


J Clin Microbiol


Luker G,Chow C,Richards DF,Johnson FB





Has Abstract


1991-07-01 00:00:00












  • Rapid speciation of Haemophilus with the porphyrin production test versus the satellite test for X.

    abstract::The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more ra...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lund ME,Blazevic DJ

    更新日期:1977-02-01 00:00:00

  • Simultaneous runs of the Bayer VERSANT HIV-1 version 3.0 and HCV bDNA version 3.0 quantitative assays on the system 340 platform provide reliable quantitation and improved work flow.

    abstract::Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Elbeik T,Markowitz N,Nassos P,Kumar U,Beringer S,Haller B,Ng V

    更新日期:2004-07-01 00:00:00

  • Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens.

    abstract::Specimens of colonic mucosa from two pigs with diarrhea were examined by light and electron microscopy. The epithelial surfaces of both pigs were extensively colonized by large spirochetes morphologically compatible with Treponema hyodysenteriae or Treponema innocens. The microorganisms were intimately attached end-on...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jacques M,Girard C,Higgins R,Goyette G

    更新日期:1989-05-01 00:00:00

  • Streamlining HIV testing for HIV preexposure prophylaxis.

    abstract::HIV-testing algorithms for preexposure prophylaxis (PrEP) should be optimized to minimize the risk of drug resistance, the time off PrEP required to evaluate false-positive screening results, and costs and to expedite the start of therapy for those confirmed to be infected. HIV rapid tests (RTs) for anti-HIV antibodie...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Guanira JV,Leigler T,Kallas E,Schechter M,Sharma U,Glidden D,Grant RM,iPrEx Study Team.

    更新日期:2015-01-01 00:00:00

  • Relationships among the results of coagulase, staphylococcal toxin, and thermonuclease tests on staphylococci from cow milk.

    abstract::Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jasper DE,Infante F,Dellinger JD

    更新日期:1985-04-01 00:00:00

  • Simultaneous Identification of Multiple β-Lactamases in Acinetobacter baumannii in Relation to Carbapenem and Ceftazidime Resistance, Using Liquid Chromatography-Tandem Mass Spectrometry.

    abstract::Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Trip H,Mende K,Majchrzykiewicz-Koehorst JA,Sedee NJ,Hulst AG,Jansen HJ,Murray CK,Paauw A

    更新日期:2015-06-01 00:00:00

  • Phylogenetic analyses of the VP4 and VP7 genes of porcine group A rotaviruses in Sao Paulo State, Brazil: first identification of G5P[23] in piglets.

    abstract::This study determined the group A rotavirus occurrence in pig farms from 7 different cities in São Paulo State, Brazil. Out of 143 samples, 70 tested positive. Sequence analyses of 37 strains indicated that the strains had the G3, G5, G9, and P[6], P[13]/P[22]-like, and P[23] genotypes. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tonietti PO,Hora AS,Silva FD,Ruiz VL,Gregori F

    更新日期:2013-08-01 00:00:00

  • Genetic diversity and dynamic distribution of Mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in Thailand.

    abstract::This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

    更新日期:2014-12-01 00:00:00

  • Investigation of apparent false-positive urine latex particle agglutination tests for the detection of group B streptococcus antigen.

    abstract::In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Harris MC,Deuber C,Polin RA,Nachamkin I

    更新日期:1989-10-01 00:00:00

  • Mechanism of cross-contamination of blood culture bottles in outbreaks of pseudobacteremia associated with nonsterile blood collection tubes.

    abstract::Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

    更新日期:1985-07-01 00:00:00

  • Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes.

    abstract::Giardia spp. are waterborne organisms that are the most commonly identified pathogenic intestinal protozoans in the United States. Current detection techniques for Giardia species in water include microscopy and immunofluorescence techniques. Species of the genus Giardia are classified on the basis of taxonomic criter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mahbubani MH,Bej AK,Perlin MH,Schaefer FW 3rd,Jakubowski W,Atlas RM

    更新日期:1992-01-01 00:00:00

  • Evaluation of Trichinella spiralis larva group 1 antigens for serodiagnosis of human trichinellosis.

    abstract::To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

    更新日期:2004-09-01 00:00:00

  • Microculture assay for isolation of human immunodeficiency virus type 1 and for titration of infected peripheral blood mononuclear cells.

    abstract::To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Dimitrov DH,Melnick JL,Hollinger FB

    更新日期:1990-04-01 00:00:00

  • Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.

    abstract::Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zanoni R,Krieg A,Peterhans E

    更新日期:1989-03-01 00:00:00

  • Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium tuberculosis isolates with five or fewer copies of IS6110.

    abstract::The use of IS6110 as a marker for molecular epidemiological studies is limited when a Mycobacterium tuberculosis isolate has five or fewer copies of IS6110. Restriction fragment length polymorphism analysis with a highly polymorphic GC-rich repetitive sequence located in the plasmid pTBN12 (PGRS RFLP) and spoligotypin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Flores L,Jarlsberg LG,Kim EY,Osmond D,Grinsdale J,Kawamura M,Desmond E,Hopewell PC,Kato-Maeda M

    更新日期:2010-02-01 00:00:00

  • Genetic heterogeneity among strains of Treponema phagedenis-like spirochetes isolated from dairy cattle with papillomatous digital dermatitis in Japan.

    abstract::Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Yano T,Yamagami R,Misumi K,Kubota C,Moe KK,Hayashi T,Yoshitani K,Ohtake O,Misawa N

    更新日期:2009-03-01 00:00:00

  • Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis.

    abstract::Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrap...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ramírez JR,Agudelo S,Muskus C,Alzate JF,Berberich C,Barker D,Velez ID

    更新日期:2000-10-01 00:00:00

  • Comparison of the INNO-LiPA and PapType assays for detection of human papillomavirus in archival vulva dysplasia and/or neoplasia tissue biopsy specimens.

    abstract::INNO-LiPA and PapType human papillomavirus (HPV) genotyping assays were compared for detection of HPV genotypes on archival vulvar tissue. The INNO-LiPA assay detected 49 HPV-16 infections, compared with 47 detected by the PapType assay. The INNO-LiPA assay detected amplifiable DNA in 59 (91%) biopsy specimens, compar...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tan SE,Garland SM,Rumbold AR,Tabrizi SN

    更新日期:2011-11-01 00:00:00

  • Quantitative estimation by a standardized enzyme-linked immunosorbent assay of human T-cell lymphotropic virus type I antibodies in adult T-cell leukemia and acquired immune deficiency syndrome.

    abstract::Sera from patients with adult T-cell leukemia and asymptomatic carriers of human T-cell lymphotropic virus type I (HTLV-I) from widely separated areas of the world reacted strongly in a standardized quantitative enzyme-linked immunosorbent assay procedure with HTLV-I viral antigen prepared from a strain isolated in th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Halbert SP,Poiesz B,Friedman-Kien AE,Montagna R,Blattner WA,Anken M

    更新日期:1986-02-01 00:00:00

  • Five cases of Haemophilus segnis appendicitis.

    abstract::The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Welch WD,Southern PM Jr,Schneider NR

    更新日期:1986-11-01 00:00:00

  • Breakthrough Hormographiella aspergillata infections arising in neutropenic patients treated empirically with caspofungin.

    abstract::Hormographiella aspergillata, a filamentous basidiomycete, has rarely been involved in human infections. We describe 2 febrile neutropenic patients who developed a severe pulmonary infection due to H. aspergillata while receiving empirical caspofungin therapy for presumed fungal pneumonia. After introduction of liposo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Suarez F,Olivier G,Garcia-Hermoso D,Randriamalala E,Ghez D,Bruneau J,Kauffmann-Lacroix C,Bougnoux ME,Lortholary O

    更新日期:2011-01-01 00:00:00

  • Opportunistic infection of the spleen caused by Aureobasidium pullulans.

    abstract::The mold Aureobasidium pullulans was isolated on several nutrient media from a splenic abscess in a patient with disseminated lymphoma. Examination of stained smears and paraffin sections revealed fungal structures characteristic of this organism. This is the first reported association of A. pullulans with an opportun...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Salkin IF,Martinez JA,Kemna ME

    更新日期:1986-05-01 00:00:00

  • Identification of newly described Streptococcus pneumoniae serotype 6D by use of the Quellung reaction and PCR.

    abstract::We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identif...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Oftadeh S,Satzke C,Gilbert GL

    更新日期:2010-09-01 00:00:00

  • Comparison of cytomegalovirus loads in plasma and leukocytes of patients with cytomegalovirus retinitis. The Cytomegalovirus Retinitis and Viral Resistance Study Group.

    abstract::Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jabs DA,Forman M,Enger C,Jackson JB

    更新日期:1999-05-01 00:00:00

  • Fluorescent pseudomonads capable of growth at 41 degrees C but distinct from Pseudomonas aeruginosa.

    abstract::One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ajello GW,Hoadley AW

    更新日期:1976-11-01 00:00:00

  • Detection of poliovirus antigen by enzyme immunoassay.

    abstract::A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ukkonen P,Huovilainen A,Hovi T

    更新日期:1986-12-01 00:00:00

  • Antibodies to various bacterial cell wall peptidoglycans in human and rabbit sera.

    abstract::Sera from patients with verified systemic staphylococcal infection contained antibodies reactive with peptidoglycan (PG) from Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Micrococcus lysodeikticus, Bacillus subtilis, and Escherichia coli. The presence of anti-PG cross-reactive antibodies ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wergeland HI,Endresen C

    更新日期:1987-03-01 00:00:00

  • Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens.

    abstract::A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conse...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nix WA,Oberste MS,Pallansch MA

    更新日期:2006-08-01 00:00:00

  • Characterization of Clostridium difficile strains isolated from patients in Ontario, Canada, from 2004 to 2006.

    abstract::Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

    更新日期:2008-09-01 00:00:00

  • DNA macroarray for identification and typing of Staphylococcus aureus isolates.

    abstract::A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

    更新日期:2004-05-01 00:00:00