Suitability of infection of cells in suspension for detection of herpes simplex virus.

Abstract:

:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, depending on the cell line used in the tests. The mean time to diagnosis by the suspension-infection technique was 1 day, compared with 4.8 days by conventional culture. Comparable detection of low-level positive specimens was observed with the methods. In a clinical setting, the isolation rates obtained by suspension-infection and conventional culture were indistinguishable. These results indicate that the suspension-infection method can be used for the detection of herpes simplex virus and can yield rapid diagnostic results without a time-consuming centrifugation step.

journal_name

J Clin Microbiol

authors

Luker G,Chow C,Richards DF,Johnson FB

doi

10.1128/JCM.29.7.1554-1557.1991

keywords:

subject

Has Abstract

pub_date

1991-07-01 00:00:00

pages

1554-7

issue

7

eissn

0095-1137

issn

1098-660X

journal_volume

29

pub_type

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