Abstract:
:A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified in vitro RNA as a competitor in a single-tube NASBA reaction. Parasite densities ranging from 10 to 10(8) Plasmodium falciparum parasites per ml could be demonstrated and quantified in whole blood. This is approximately 1,000 times more sensitive than conventional microscopy analysis of thick blood smears. Comparison of the parasite densities obtained by microscopy and QT-NASBA with 120 blood samples from Kenyan patients with clinical malaria revealed that for 112 of 120 (93%) of the samples results were within a 1-log difference. QT-NASBA may be especially useful for the detection of low parasite levels in patients with early-stage malaria and for the monitoring of the efficacy of drug treatment.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Schoone GJ,Oskam L,Kroon NC,Schallig HD,Omar SAdoi
10.1128/JCM.38.11.4072-4075.2000subject
Has Abstractpub_date
2000-11-01 00:00:00pages
4072-5issue
11eissn
0095-1137issn
1098-660Xjournal_volume
38pub_type
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