Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification.

Abstract:

:A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified in vitro RNA as a competitor in a single-tube NASBA reaction. Parasite densities ranging from 10 to 10(8) Plasmodium falciparum parasites per ml could be demonstrated and quantified in whole blood. This is approximately 1,000 times more sensitive than conventional microscopy analysis of thick blood smears. Comparison of the parasite densities obtained by microscopy and QT-NASBA with 120 blood samples from Kenyan patients with clinical malaria revealed that for 112 of 120 (93%) of the samples results were within a 1-log difference. QT-NASBA may be especially useful for the detection of low parasite levels in patients with early-stage malaria and for the monitoring of the efficacy of drug treatment.

journal_name

J Clin Microbiol

authors

Schoone GJ,Oskam L,Kroon NC,Schallig HD,Omar SA

doi

10.1128/JCM.38.11.4072-4075.2000

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

4072-5

issue

11

eissn

0095-1137

issn

1098-660X

journal_volume

38

pub_type

杂志文章
  • Development and Validation of the Elecsys Anti-SARS-CoV-2 Immunoassay as a Highly Specific Tool for Determining Past Exposure to SARS-CoV-2.

    abstract::The Elecsys Anti-SARS-CoV-2 immunoassay (Roche Diagnostics) was developed to provide accurate, reliable detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutraliz...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01694-20

    authors: Muench P,Jochum S,Wenderoth V,Ofenloch-Haehnle B,Hombach M,Strobl M,Sadlowski H,Sachse C,Torriani G,Eckerle I,Riedel A

    更新日期:2020-09-22 00:00:00

  • Sequencing-based genotyping of mixed human papillomavirus infections by use of RipSeq software.

    abstract::Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00091-13

    authors: Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

    更新日期:2013-04-01 00:00:00

  • Enhanced recovery of cytomegalovirus in conventional tube cultures with a spin-amplified adsorption.

    abstract::Low-speed centrifugation-mediated adsorption was evaluated as an enhancement of infectivity of clinical and laboratory strains of cytomegalovirus (CMV) occurring with cells grown in conventional culture tubes. The time required for reporting of primary isolates of CMV from urine specimens adsorbed onto monolayers of W...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.5.965-969.1990

    authors: Oefinger PE,Shawar RM,Loo SH,Tsai LT,Arnett JK

    更新日期:1990-05-01 00:00:00

  • Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae.

    abstract::We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.2.660-662.2002

    authors: Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

    更新日期:2002-02-01 00:00:00

  • The Search for a Practical Method for Colistin Susceptibility Testing: Have We Found It by Going Back to the Future?

    abstract::Polymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this i...

    journal_title:Journal of clinical microbiology

    pub_type: 评论,杂志文章

    doi:10.1128/JCM.01608-18

    authors: Satlin MJ

    更新日期:2019-01-30 00:00:00

  • Molecular Testing for Plasmodium falciparum by Use of Serum or Plasma and Comparison with Microscopy and Rapid Diagnostic Testing in Febrile Nigerian Patients.

    abstract::Plasmodium nucleic acids have been detected in serum and plasma, but there is little published data describing the diagnostic performance of malaria nucleic acid amplification tests (NAATs) using these specimen types. Previously, our group described a multiplex NAAT for the detection of dengue virus, Leptospira, and P...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01876-15

    authors: Waggoner JJ,Okangba C,Mohamed-Hadley A,Lefterova MI,Banaei N,Oyibo W,Pinsky BA

    更新日期:2015-11-01 00:00:00

  • Characterization and distribution of Pasteurella species recovered from infected humans.

    abstract::During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.11.2984-2987.1992

    authors: Holst E,Rollof J,Larsson L,Nielsen JP

    更新日期:1992-11-01 00:00:00

  • Detection of binding antibodies to native and recombinant human immunodeficiency virus type 1 envelope glycoproteins following recombinant gp160 immunization measured by flow cytometry and enzyme immunoassays. The AIDS Vaccine Clinical Trials Network.

    abstract::The ability of antibody induced by vaccination with recombinant gp160 (rgp160) to bind to native and recombinant human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins was measured. Thirty-three HIV-1-seronegative healthy adult volunteers were injected four times with 40 or 80 micrograms of an HIV-1LAV env...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.10.2606-2612.1992

    authors: Gorse GJ,Frey SE,Newman FK,Belshe RB

    更新日期:1992-10-01 00:00:00

  • A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay.

    abstract::Rapid tests for detection of methicillin-resistant Staphylococcus aureus (MRSA) carriage are important to limit the transmission of MRSA in the health care setting. We evaluated the performance of the BD GeneOhm MRSA real-time PCR assay using a diverse collection of MRSA isolates, mainly from Copenhagen, Denmark, but ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02153-08

    authors: Bartels MD,Boye K,Rohde SM,Larsen AR,Torfs H,Bouchy P,Skov R,Westh H

    更新日期:2009-05-01 00:00:00

  • Performance characteristics of a commercially prepared biphasic blood culture bottle.

    abstract::A biphasic blood culture bottle (BiPB: GIBCO Laboratories, North Andover, Mass.) with an architectural design that physically separates the agar slant from the broth was compared with a conventional vented monophasic bottle (MPB-A) for use in the routine culture of blood. Both bottles contained tryptic soy broth. Tryp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.4.700-703.1986

    authors: Weckbach LS,Staneck JL

    更新日期:1986-04-01 00:00:00

  • Preliminary evaluation of a rapid colorimetric method for identification of pathogenic Neisseria.

    abstract::A rapid colorimetric method for the identification of pathogenic Neisseria (Identicult-Neisseria; Scott Laboratories, Inc.) based on beta-galactosidase, gamma-glutamylaminopeptidase, and gamma-prolylaminopeptidase is described. All 82 clinical isolates of Neisseria gonorrhoeae, 9 clinical isolates of N. meningitidis, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.1.141-142.1986

    authors: Hosmer ME,Cohenford MA,Ellner PD

    更新日期:1986-07-01 00:00:00

  • Sequence polymorphisms in a surface PPE protein distinguish types I, II, and III of Mycobacterium avium subsp. paratuberculosis.

    abstract::In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02031-07

    authors: Griffiths TA,Rioux K,De Buck J

    更新日期:2008-04-01 00:00:00

  • The rise of Tropheryma whipplei: a 12-year retrospective study of PCR diagnoses in our reference center.

    abstract::Tropheryma whipplei is the causative agent of classic Whipple's disease (WD) and other clinical entities, such as localized infection. Asymptomatic carriers have also been reported, mainly based on the testing of fecal samples. Our objective was to undertake a retrospective analysis of molecular biology usage for the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01517-12

    authors: Edouard S,Fenollar F,Raoult D

    更新日期:2012-12-01 00:00:00

  • Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.

    abstract::Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one comme...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02251-14

    authors: Ye S,Lambert SB,Grimwood K,Roczo-Farkas S,Nimmo GR,Sloots TP,Kirkwood CD,Whiley DM

    更新日期:2015-01-01 00:00:00

  • Detection of cytomegalovirus antibody with latex agglutination.

    abstract::Transfusion-acquired cytomegalovirus (CMV) infections should be prevented in seronegative immunocompromised patients by providing blood products from donors who are also seronegative. Latex agglutination was investigated as a simple and rapid method for detecting antibody against CMV. Latex beads were coated with CMV ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.1.68-70.1985

    authors: Adler SP,McVoy M,Biro VG,Britt WJ,Hider P,Marshall D

    更新日期:1985-07-01 00:00:00

  • Proficiency analysis of drug susceptibility testing by national-level tuberculosis reference laboratories from 1995 to 2003.

    abstract::A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00784-07

    authors: Bai GH,Kim SJ,Chang CL,National and Regional Tuberculosis Reference Laboratories.

    更新日期:2007-11-01 00:00:00

  • Serodiagnosis of acute hepatitis B virus infection by a modified competitive binding radioimmunoassay.

    abstract::Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.5.973-975.1982

    authors: Schable CA,Maynard JE

    更新日期:1982-11-01 00:00:00

  • Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization.

    abstract::The present report describes an analysis of two virulence genes of Helicobacter pylori. Parts of the cagA gene, as well as parts from the signal (s) and middle (m) regions of the mosaic vacA gene, were amplified with biotin-labelled PCR primers and the products were subsequently analyzed by a single-step reverse hybri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.5.1271-1276.1998

    authors: van Doorn LJ,Figueiredo C,Rossau R,Jannes G,van Asbroek M,Sousa JC,Carneiro F,Quint WG

    更新日期:1998-05-01 00:00:00

  • Comparison of techniques for recovering murine cytomegalovirus from a macrophage-enriched subpopulation of mice.

    abstract::We previously demonstrated the transmission of murine cytomegalovirus to syngeneic mice and preformed monolayers of mouse embryo fibroblasts with plastic-adherent peritoneal exudate cells from infected mice as a source of macrophages. In the present studies we compared this standard feeder layer method with a reverse ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.6.785-789.1980

    authors: Winkler SR,Booss J

    更新日期:1980-12-01 00:00:00

  • Aspergillus citrinoterreus, a new species of section Terrei isolated from samples of patients with nonhematological predisposing conditions.

    abstract::The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03088-14

    authors: Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

    更新日期:2015-02-01 00:00:00

  • Direct fluorescent-antibody testing followed by culture for diagnosis of 2009 H1N1 influenza A.

    abstract::During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.05255-11

    authors: Bakerman P,Balasuriya L,Fried O,Tellez D,Garcia-Filion P,Dalton H

    更新日期:2011-10-01 00:00:00

  • Use of nasopharyngeal aspirate for diagnosis of pneumocystis pneumonia.

    abstract::Quantitative PCR on nasopharyngeal aspirate (NPA) can achieve high sensitivity and specificity in diagnosing Pneumocystis pneumonia (PCP) compared to microscopic examination of bronchoscopic specimens in a population with low HIV prevalence. Since NPA is a minimally invasive procedure, it is ideal as a screening test ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03264-12

    authors: To KK,Wong SC,Xu T,Poon RW,Mok KY,Chan JF,Cheng VC,Chan KH,Hung IF,Yuen KY

    更新日期:2013-05-01 00:00:00

  • Irreproducible and uninterpretable Polymyxin B MICs for Enterobacter cloacae and Enterobacter aerogenes.

    abstract::Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02129-13

    authors: Landman D,Salamera J,Quale J

    更新日期:2013-12-01 00:00:00

  • Recurrent bacteremic peritonitis caused by Enterococcus cecorum in a patient with liver cirrhosis.

    abstract::Enterococcus cecorum (formerly Streptococcus cecorum), originally isolated from poultry intestines, has rarely been encountered in human diseases. A 60-year-old man with liver cirrhosis and hepatocellular carcinoma developed peritonitis on the seventh day of his hospitalization. Cultures of one blood sample and one as...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Hsueh PR,Teng LJ,Chen YC,Yang PC,Ho SW,Luh KT

    更新日期:2000-06-01 00:00:00

  • Evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species.

    abstract::A total of 410 well-defined Campylobacter, Helicobacter, and Wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the Centers for Disease Control, Atlanta, Ga. All C. coli (43 strains), C. cryaerophila (34...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.10.2335-2339.1990

    authors: Popovic-Uroic T,Patton CM,Nicholson MA,Kiehlbauch JA

    更新日期:1990-10-01 00:00:00

  • Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps).

    abstract::Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02206-16

    authors: Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

    更新日期:2017-03-01 00:00:00

  • Use of a simple separation column in detection of immunoglobulin M antibody to Epstein-Barr virus.

    abstract::The use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin M antibody to Epstein-Barr virus. The efficiency of this product in absorbing interfering immunoglobulin G was similar to that of another absorption product, staphylococcal protein A, but it has the advan...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.2.298-299.1984

    authors: Sumaya CV,Ench Y,Carrillo MA,Pope RM

    更新日期:1984-08-01 00:00:00

  • Three new serotypes of Salmonella.

    abstract::Three new Salmonella serotypes belonging to Kauffmann's subgenus I (F. Kauffmann, The Bacteriology of Enterobacteriaceae, 1966) were identified. These serotypes were Salmonella brazos 6,14,18:a:e,n,z15, Salmonella midway 6,14,24:d:1,7, and Salmonella balboa 48a, 48b:z41:monophasic. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.6.748-749.1980

    authors: Sutch KE,Blackburn BO

    更新日期:1980-06-01 00:00:00

  • Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant enterococci.

    abstract::We are reporting on a nosocomial outbreak of 213 cases of vancomycin-resistant enterococcus infection involving 2,812 enterococcal isolates from patients over a period of 36 months. In 1990, the Enterococcus faecium vancomycin susceptibility rate was found to be 85.7% (36 of 42 cases), and an incidence of 10.9% (42 of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.5.1280-1285.1993

    authors: Boyle JF,Soumakis SA,Rendo A,Herrington JA,Gianarkis DG,Thurberg BE,Painter BG

    更新日期:1993-05-01 00:00:00

  • Multicenter performance evaluation of a new TaqMan PCR assay for monitoring human immunodeficiency virus RNA load.

    abstract::A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with the licensed COBAS AMPLICOR HIV-1 MONITOR (CAHIM) assay. In this study, we have analyzed the tests' sensitivi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00505-06

    authors: Perrin L,Pawlotsky JM,Bouvier-Alias M,Sarrazin C,Zeuzem S,Colucci G

    更新日期:2006-12-01 00:00:00