Involvement of streptococcal mitogenic exotoxin Z in streptococcal toxic shock syndrome.

abstract：:The performance of a disk diffusion test using broth from positive blood cultures as inoculum (direct disk diffusion [dDD]) was evaluated for a collection of 20 challenge isolates of Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa Isolates seeded into human blood were inoculated into Bactec Plu...

journal_title：Journal of clinical microbiology

authors： Chandrasekaran S,Abbott A,Campeau S,Zimmer BL,Weinstein M,Thrupp L,Hejna J,Walker L,Ammann T,Kirn T,Patel R,Humphries RM

更新日期：2018-02-22 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2010-05-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

journal_title：Journal of clinical microbiology

authors： Medina SM,Gutierrez MF,Liprandi F,Ludert JE

更新日期：2000-09-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:We describe the first case of Epstein-Barr virus (EBV)-associated thymic carcinoid tumor found by in situ hybridization (ISH) on paraffin-embedded sections. ISH revealed that both tumor cells and infiltrated lymphocytes were EBV positive, while a few EBV-infected lymphocytes were detected in 2 of 11 thymuses and 1 of ...

journal_title：Journal of clinical microbiology

authors： Takeuchi H,Fujita H,Iwasaki F,Takeuchi T,Imadome K,Okumiya T,Matsui T,Takahashi M

更新日期：2004-06-01 00:00:00

abstract：:A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reasses...

journal_title：Journal of clinical microbiology

authors： Kondas AV,Olson VA,Li Y,Abel J,Laker M,Rose L,Wilkins K,Turner J,Kline R,Damon IK

更新日期：2015-04-01 00:00:00

abstract：:The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of...

journal_title：Journal of clinical microbiology

authors： Pasteran F,Mendez T,Guerriero L,Rapoport M,Corso A

更新日期：2009-06-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysi...

journal_title：Journal of clinical microbiology

authors： Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PD

更新日期：1981-09-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...

journal_title：Journal of clinical microbiology

authors： Hakim H,Gibson C,Pan J,Srivastava K,Gu Z,Bankowski MJ,Hayden RT

更新日期：2007-07-01 00:00:00

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:Treatment of patients with chronic hepatitis B virus (HBV) infections with potent antiviral therapy often results in dramatic reductions in the levels of viremia to very low levels. Monitoring of serum HBV DNA levels is a consistent method for the assessment of antiviral potency; however, widely used hybridization ass...

journal_title：Journal of clinical microbiology

authors： Lopez VA,Bourne EJ,Lutz MW,Condreay LD

更新日期：2002-06-01 00:00:00

abstract：:An in vivo model of hub-related catheter sepsis in rabbits is reported. The model was used to investigate the protection offered by a new hub design against external contamination by Pseudomonas aeruginosa or Staphylococcus epidermidis and to reassess the diagnostic value of the semiquantitative culture method in bact...

journal_title：Journal of clinical microbiology

authors： Segura M,Alía C,Valverde J,Franch G,Torres Rodríguez JM,Sitges-Serra A

更新日期：1990-11-01 00:00:00

abstract：:A case of severe progressive subcutaneous abscesses and necrotizing tenosynovitis of the right arm of a 30-year-old woman caused by Rhodococcus aurantiacus is reported. ...

journal_title：Journal of clinical microbiology

authors： Tsukamura M,Hikosaka K,Nishimura K,Hara S

更新日期：1988-02-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from genome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), th...

journal_title：Journal of clinical microbiology

authors： Aradaib IE,Wilson WC,Schore CE,Mohammed ME,Yilma TD,Cullor JS,Osburn BI

更新日期：1998-09-01 00:00:00

abstract：:We compared the efficacy of human papillomavirus (HPV) DNA detection between a PCR-based genechip (Easychip HPV Blot [hereafter referred to as HPV Blot]; King Car, Taiwan) method and Hybrid Capture II (HCII; Digene, Gaithersburg, MD) in women with previous normal (n = 146) or abnormal (> or =atypical squamous cells of...

journal_title：Journal of clinical microbiology

authors： Huang SL,Chao A,Hsueh S,Chao FY,Huang CC,Yang JE,Lin CY,Yan CC,Chou HH,Huang KG,Huang HJ,Wu TI,Tseng MJ,Qiu JT,Lin CT,Chang TC,Lai CH

更新日期：2006-05-01 00:00:00

abstract：:A commercial DNA probe kit designed to detect rRNA from legionellae was evaluated for its ability to correctly discriminate between legionellae and non-legionellae taken from culture plates. The probe kit, made by the Gen-Probe Corp. (San Diego, Calif.), was radiolabeled with 125I, and probe bacterial RNA hybridizatio...

journal_title：Journal of clinical microbiology

authors： Edelstein PH

更新日期：1986-03-01 00:00:00

abstract：:The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk patients were paired with subsequent bloodstream isolates. Organisms were typed by using restriction endonuclease digest...

journal_title：Journal of clinical microbiology

authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

更新日期：1990-12-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for...

journal_title：Journal of clinical microbiology

authors： Bardell D

更新日期：1975-02-01 00:00:00

abstract：:Of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (Ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (Ad1 in two cases, Ad18 or Ad31) which was selected over Ad40 during serial passage in the 293 cells. The selection of Ad1 over Ad40...

journal_title：Journal of clinical microbiology

authors： Brown M

更新日期：1985-08-01 00:00:00

abstract：:In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

journal_title：Journal of clinical microbiology

authors： Ghannoum MA,Rex JH,Galgiani JN

更新日期：1996-03-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay ...

journal_title：Journal of clinical microbiology

authors： Seno M,Kanamoto Y,Takao S,Takei N,Fukuda S,Umisa H

更新日期：1990-07-01 00:00:00