Abstract:
:A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysis in polyacrylamide-agarose composite gels. The rotaviral genome electrophoretic migration pattern produced by double-stranded RNA extracted directly from a specimen by this procedure was qualitatively identical to the electrophoretic migration pattern obtained with double-stranded RNA extracted from purified rotavirus derived from the same specimen. Direct extraction of specimens containing porcine rotavirus-like virus by this procedure gave preparations that had electrophoretic migration patterns similar, but not identical, to the characteristic electrophoretic migration pattern of the rotaviral genome. Sufficient rotaviral double-stranded RNA could be extracted from 6 ml of fecal or intestinal specimen by this procedure to permit 15 or more electrophoretic assays.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PDdoi
10.1128/JCM.14.3.273-280.1981subject
Has Abstractpub_date
1981-09-01 00:00:00pages
273-80issue
3eissn
0095-1137issn
1098-660Xjournal_volume
14pub_type
杂志文章abstract::A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...
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journal_title:Journal of clinical microbiology
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pub_type: 杂志文章,多中心研究
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更新日期:1999-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JCM.32.5.1406-1407.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1990-10-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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