Abstract:
:Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine tuberculosis since 1997 by the EU, M. bovis infections still occur sporadically in cattle and other mammals, including humans. Here, the transmission routes of a bTB outbreak in a wildlife park in Germany affecting different cervid species, bison, lynx, and pot-bellied pigs were followed by employing whole-genome sequencing (WGS) combined with spoligotyping and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing. One single M. bovis strain persisted from 2002 to 2015, and transmission between the park and a distantly located captive cervid farm was verified. The spoligotyping patterns remained identical, while MIRU-VNTR typing of 24 loci of the standardized panel and locus 2163a as an additional locus revealed one change at locus 2165 in a strain from a fallow deer and one at locus 2461 in isolates from red deer over the whole time period. WGS analysis confirmed the close relatedness of the isolates, with a maximum of 12 single nucleotide polymorphisms (SNPs) detected between any two sequenced isolates. In conclusion, our data confirm a longitudinal outbreak of M. bovis in a German wildlife park and provide the first insights into the dynamics of different genotyping markers in M. bovis.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Kohl TA,Utpatel C,Niemann S,Moser Idoi
10.1128/JCM.00302-18subject
Has Abstractpub_date
2018-08-27 00:00:00issue
9eissn
0095-1137issn
1098-660Xpii
JCM.00302-18journal_volume
56pub_type
杂志文章abstract::A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.8.2346-2348.1998
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.2.287-291.1995
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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更新日期:1983-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.11.2873-2877.1993
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pub_type: 杂志文章,多中心研究
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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更新日期:1982-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.2.245-247.1984
更新日期:1984-02-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.8.2016-2021.1995
更新日期:1995-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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