Rapid identification of antibiotic-resistant corynebacteria with the API 20S system.

Abstract:

:The API 20S system (Analytab Products, Plainview, N.Y.) was evaluated for the rapid identification of multiply antibiotic-resistant aerobic diphtheroids. Sixty-eight clinical isolates of multiply resistant Centers for Disease Control group JK and group D2 corynebacteria had API 20S profiles which were clearly different from those of a number of strains of other Corynebacterium species which were tested. The API 20S system allowed more rapid identification of antibiotic-resistant diphtheroids than conventional biochemical tests. Its use for corynebacteria other than group JK and group D2 is not recommended at this time.

journal_name

J Clin Microbiol

authors

Kelly MC,Smith ID,Anstey RJ,Thornley JH,Rennie RP

doi

10.1128/JCM.19.2.245-247.1984

subject

Has Abstract

pub_date

1984-02-01 00:00:00

pages

245-7

issue

2

eissn

0095-1137

issn

1098-660X

journal_volume

19

pub_type

杂志文章
  • Molecular epidemiology of an outbreak caused by methicillin-resistant Staphylococcus aureus in a health care ward and associated nursing home.

    abstract::Our point-prevalence survey followed an outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in a long-term care facility and identified five MRSA strains, of which two possessed an outbreak genotype not encountered previously and three had another profile. All of them possessed SCCmec type V. Six methicilli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.12.6161-6163.2005

    authors: Kerttula AM,Lyytikäinen O,Vuopio-Varkila J,Ibrahem S,Agthe N,Broas M,Jägerroos H,Virolainen A

    更新日期:2005-12-01 00:00:00

  • Molecular detection and epidemiology of Sapporo-like viruses.

    abstract::Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.2.530-536.2000

    authors: Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

    更新日期:2000-02-01 00:00:00

  • Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens.

    abstract::Specimens of colonic mucosa from two pigs with diarrhea were examined by light and electron microscopy. The epithelial surfaces of both pigs were extensively colonized by large spirochetes morphologically compatible with Treponema hyodysenteriae or Treponema innocens. The microorganisms were intimately attached end-on...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.5.1139-1141.1989

    authors: Jacques M,Girard C,Higgins R,Goyette G

    更新日期:1989-05-01 00:00:00

  • Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting.

    abstract::Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.8.2198-2200.1993

    authors: van Belkum A,Struelens M,Quint W

    更新日期:1993-08-01 00:00:00

  • Comparison of Manual and Fully Automated AIX1000 Rapid Plasma Reagin Assays for Laboratory Diagnosis of Syphilis.

    abstract::The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00214-18

    authors: Sanfilippo AM,Freeman K,Schmitz JL

    更新日期:2018-07-26 00:00:00

  • Use of Automated Urine Microscopy Analysis in Clinical Diagnosis of Urinary Tract Infection: Defining an Optimal Diagnostic Score in an Academic Medical Center Population.

    abstract::A retrospective case record study was conducted that established a scoring tool based on clinical and iQ200 parameters, able to predict or rule out the clinical diagnosis of UTI in the majority of adult patients in an academic hospital. Automated standardized quantitative urine analysis, such as iQ200 analysis, is on ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02030-17

    authors: Foudraine DE,Bauer MP,Russcher A,Kusters E,Cobbaert CM,van der Beek MT,Stalenhoef JE

    更新日期:2018-05-25 00:00:00

  • Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats.

    abstract::Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.5.1159-1165.1994

    authors: Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

    更新日期:1994-05-01 00:00:00

  • Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India.

    abstract::Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.6.1592-1594.1996

    authors: Husain M,Seth P,Dar L,Broor S

    更新日期:1996-06-01 00:00:00

  • Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide ligation assay on DNA chips.

    abstract::A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.10.4850-4852.2004

    authors: Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

    更新日期:2004-10-01 00:00:00

  • Evaluation of confirmatory strategies for detection of type-specific antibodies against herpes simplex virus type 2.

    abstract::In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.2.407-413.2002

    authors: Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

    更新日期:2002-02-01 00:00:00

  • Effect of atypical antibiotic resistance on microorganism identification by pattern recognition.

    abstract::We classified microorganisms from the clinical laboratory by using information provided by the Gram stain and antibiotic sensitivity profiles obtained with the Bauer-Kirby technique. Approximately 4,000 microorganisms, routinely identified and tested for antibiotic sensitivities in a large hospital microbiology labora...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Boyd JC,Lewis JW,Marr JJ,Harper AM,Kowalski BR

    更新日期:1978-12-01 00:00:00

  • Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.

    abstract::A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00064-13

    authors: Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

    更新日期:2013-06-01 00:00:00

  • Clinical laboratory evaluation of a urine screening device.

    abstract::A study was conducted to compare the Bac-T-Screen Bacterial Detection Device for Urines (BDD; Marion Laboratories, Kansas City, Mo.) with urine Gram stain as a screen for bacteriuria. We analyzed 631 urine samples with the BDD and compared the results to urine Gram stains and quantitative cultures. A total of 90 (14%)...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.3.674-679.1983

    authors: Pfaller MA,Baum CA,Niles AC,Murray PR

    更新日期:1983-09-01 00:00:00

  • Monitoring Shedding of Five Genotypes of RotaTeq Vaccine Viruses by Genotype-Specific Real-Time Reverse Transcription-PCR Assays.

    abstract::RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00035-18

    authors: Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

    更新日期:2018-05-25 00:00:00

  • Interpretation of spectrophotometric absorbance values to define results of enzyme-linked immunosorbent assays.

    abstract::An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.4.398-401.1980

    authors: Heck FC,Williams JD,Pruett J

    更新日期:1980-04-01 00:00:00

  • Comparative analysis of immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay using virus-like particles or virus-infected mouse brain antigens to detect IgM antibody in sera from patients with evident flaviviral infections.

    abstract::The use of immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) serves as a valuable tool for the diagnosis of acute flaviviral infections, since IgM antibody titers are detectable early, peak at about 2 weeks postinfection, and subsequently decline to lower levels over the next few mo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.7.3227-3236.2005

    authors: Holmes DA,Purdy DE,Chao DY,Noga AJ,Chang GJ

    更新日期:2005-07-01 00:00:00

  • MDCK-SIAT1 cells show improved isolation rates for recent human influenza viruses compared to conventional MDCK cells.

    abstract::The ability to isolate and propagate influenza virus is an essential tool for the yearly surveillance of circulating virus strains and to ensure accurate clinical diagnosis for appropriate treatment. The suitability of MDCK-SIAT1 cells, engineered to express increased levels of alpha-2,6-linked sialic acid receptors, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00398-08

    authors: Oh DY,Barr IG,Mosse JA,Laurie KL

    更新日期:2008-07-01 00:00:00

  • Detection of circulating free and complexed staphylococcal antigens by enzyme-linked immunosorbent assay.

    abstract::An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.6.1019-1024.1982

    authors: Lentino JR,Rytel MW

    更新日期:1982-12-01 00:00:00

  • Multilocus sequence analysis provides insights into molecular epidemiology of Chlamydia pecorum infections in Australian sheep, cattle, and koalas.

    abstract::Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed a C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00992-13

    authors: Jelocnik M,Frentiu FD,Timms P,Polkinghorne A

    更新日期:2013-08-01 00:00:00

  • Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

    abstract::A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.12.3331-3334.1997

    authors: Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

    更新日期:1997-12-01 00:00:00

  • Typing of Salmonella enterica serotype paratyphi C isolates from various countries by plasmid profiles and pulsed-field gel electrophoresis.

    abstract::Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.6.2058-2060.1999

    authors: Kariuki S,Cheesbrough J,Mavridis AK,Hart CA

    更新日期:1999-06-01 00:00:00

  • Modified method for testing the quality of albumin-containing enrichments used in growth media for mycobacteria.

    abstract::Many commercially available media for cultivation of mycobacteria have failed to support the growth of these organisms. This is especially true of media prepared with albumin-containing enrichments. Earlier, we developed a method for rapid identification of good albumin enrichments for agar-based media used to test th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.5.1068-1070.1990

    authors: Butler WR,Warren NG,Kubica GP,Kilburn JO

    更新日期:1990-05-01 00:00:00

  • Chemical decontamination with N-acetyl-L-cysteine-sodium hydroxide improves recovery of viable Mycobacterium avium subsp. paratuberculosis organisms from cultured milk.

    abstract::Mycobacterium avium subsp. paratuberculosis is shed into the milk and feces of cows with advanced Johne's disease, allowing the transmission of M. avium subsp. paratuberculosis between animals. The objective of this study was to formulate an optimized protocol for the isolation of M. avium subsp. paratuberculosis in m...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00508-13

    authors: Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

    更新日期:2013-07-01 00:00:00

  • Genetic similarity of Candida albicans strains from vaginitis patients and their partners.

    abstract::The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.1.39-46.1993

    authors: Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

    更新日期:1993-01-01 00:00:00

  • Inverse association between Lancefield group G Streptococcus colonization and sore throat in slum and nonslum settings in Brazil.

    abstract::Group G Streptococcus has been implicated as a causative agent of pharyngitis in outbreak situations, but its role in endemic disease remains elusive. We found an unexpected inverse association of Streptococcus dysgalactiae subsp. equisimilis colonization and sore throat in a study of 2,194 children of 3 to 15 years o...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02095-10

    authors: Tartof SY,Farrimond F,de Matos JA,Reis JN,Ramos RT,Andrade AN,dos Reis MG,Riley LW

    更新日期:2011-01-01 00:00:00

  • Cultivation and characterization of human rotaviruses with "super short" RNA patterns.

    abstract::Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.1.183-185.1987

    authors: Albert MJ,Unicomb LE,Bishop RF

    更新日期:1987-01-01 00:00:00

  • Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.

    abstract::We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.9.2718-2722.1998

    authors: Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

    更新日期:1998-09-01 00:00:00

  • Epidemiologic relationship between fluoroquinolone-resistant Salmonella enterica Serovar Choleraesuis strains isolated from humans and pigs in Taiwan (1997 to 2002).

    abstract::The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.6.2798-2804.2005

    authors: Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

    更新日期:2005-06-01 00:00:00

  • Detection of hepatitis C virus RNA: comparison of one-stage polymerase chain reaction (PCR) with nested-set PCR.

    abstract::We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.2.289-291.1993

    authors: Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

    更新日期:1993-02-01 00:00:00

  • Detection of Mycobacterium tuberculosis in BACTEC 12B broth cultures by the Roche Amplicor PCR assay.

    abstract::To evaluate the ability of the Amplicor MTB Assay to detect Mycobacterium tuberculosis complex (MTBC) organisms in BACTEC 12B broth cultures, 249 cultures with a growth index (GI) of > or = 20 from 160 patients were tested retrospectively. Specimens were processed by standard methods, and then BACTEC 12B vials and Mid...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.4.900-902.1997

    authors: Smith MB,Bergmann JS,Woods GL

    更新日期:1997-04-01 00:00:00