Abstract:
:The 16S rRNA gene PCR in the diagnosis of bone and joint infections has not been systematically tested. Five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16S rRNA gene PCR detection of bacteria in parallel. The amplicons with mixed sequences were also cloned. When discordant results were observed, culture and PCR were performed once again. Bacteria were detected in 139 of 525 samples. Culture and 16S rRNA gene PCR yielded identical documentation in 475 samples. Discrepancies were linked to 13 false-positive culture results, 5 false-positive PCR results, 9 false-negative PCR results, 16 false-negative culture results, and 7 mixed infections. Cloning and sequencing of 16S rRNA gene amplicons in 6 of 8 patients with mixed infections identified 2 to 8 bacteria per sample. Rarely described human pathogens such as Alcaligenes faecalis, Comamonas terrigena, and 21 anaerobes were characterized. We also detected, by 16S rRNA gene PCR, four previously identified bacteria never reported in human infection, Alkanindiges illinoisensis, dehydroabietic acid-degrading bacterium DhA-73, unidentified Hailaer soda lake bacterium, and uncultured bacterium clone HuCa4. Seven organisms representing new potential species were also detected. PCR followed by cloning and sequencing may help to identify new pathogens involved in mixed bone infection.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Fenollar F,Roux V,Stein A,Drancourt M,Raoult Ddoi
10.1128/JCM.44.3.1018-1028.2006subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1018-28issue
3eissn
0095-1137issn
1098-660Xpii
44/3/1018journal_volume
44pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.16.2.281-285.1982
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doi:10.1128/JCM.25.9.1611-1622.1987
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doi:10.1128/JCM.33.7.1791-1792.1995
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.28.1.128-130.1990
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pub_type: 杂志文章
doi:10.1128/JCM.31.6.1408-1412.1993
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.5.915-921.1989
更新日期:1989-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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