Rapid detection of blaKPC carbapenemase genes by internally controlled real-time PCR assay using bactec blood culture bottles.

abstract：:Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization-time of flight mass spectro...

journal_title：Journal of clinical microbiology

authors： Kwon YJ,Shin JH,Byun SA,Choi MJ,Won EJ,Lee D,Lee SY,Chun S,Lee JH,Choi HJ,Kee SJ,Kim SH,Shin MG

更新日期：2019-03-28 00:00:00

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...

journal_title：Journal of clinical microbiology

authors： Gunn BA

更新日期：1976-08-01 00:00:00

abstract：:To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

journal_title：Journal of clinical microbiology

authors： Everts RJ,Heneghan JP,Adholla PO,Reller LB

更新日期：2001-01-01 00:00:00

abstract：:Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specific...

journal_title：Journal of clinical microbiology

authors： Mecham JO,Wilson WC

更新日期：2004-02-01 00:00:00

abstract：:The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resu...

journal_title：Journal of clinical microbiology

authors： Vatanshenassan M,Boekhout T,Lass-Flörl C,Lackner M,Schubert S,Kostrzewa M,Sparbier K

更新日期：2018-08-27 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...

journal_title：Journal of clinical microbiology

authors： White LA,Ades EW

更新日期：1990-02-01 00:00:00

abstract：:Human papillomavirus type 16 (HPV 16) plays a cardinal role in the pathogenesis of cervical cancer. HPV 16 has intratypic variants which show different geographical distributions and different oncogenic potentials. To analyze the presence of sequence variations of HPV 16 variants in northeast China, 71 cervical carcin...

journal_title：Journal of clinical microbiology

authors： Shang Q,Wang Y,Fang Y,Wei L,Chen S,Sun Y,Li B,Zhang F,Gu H

更新日期：2011-07-01 00:00:00

abstract：:An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

journal_title：Journal of clinical microbiology

authors： Barreau C,Wagener G

更新日期：1990-08-01 00:00:00

abstract：:We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In ...

journal_title：Journal of clinical microbiology

authors： Grange PA,Jary A,Isnard C,Burrel S,Boutolleau D,Touati A,Bébéar C,Saule J,Martinet P,Robert JL,Moulene D,Vermersch-Langlin A,Benhaddou N,Janier M,Dupin N

更新日期：2021-01-21 00:00:00

abstract：:Sputum smear microscopy is an easy, inexpensive, and rapid method for detecting tubercle bacilli when there are more than 10,000 bacilli/ml in the original sputum. Furthermore, because the microscopic method provides not only quantitative, but also qualitative information, such as the shape of bacilli, it has remained...

journal_title：Journal of clinical microbiology

authors： Yamada H,Mitarai S,Wahyunitisari MR,Mertaniasih NM,Sugamoto T,Chikamatsu K,Aono A,Matsumoto H,Fujiki A

更新日期：2011-10-01 00:00:00

abstract：:A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) we...

journal_title：Journal of clinical microbiology

authors： Tang X,Zhao Z,Hu J,Wu B,Cai X,He Q,Chen H

更新日期：2009-04-01 00:00:00

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR ...

journal_title：Journal of clinical microbiology

authors： Baele M,Baele P,Vaneechoutte M,Storms V,Butaye P,Devriese LA,Verschraegen G,Gillis M,Haesebrouck F

更新日期：2000-11-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

更新日期：2004-12-01 00:00:00

abstract：:Hepatitis C virus (HCV)-positive sera from 106 chronically infected patients which had previously been genotyped were characterized by serotyping. Genotypes were determined by a first-generation line probe assay (INNO-LiPA HCV) and by sequence analyses of the core, core-E1, and NS5B regions. HCV serotypes were determi...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Kleter B,Pike I,Quint W

更新日期：1996-07-01 00:00:00

abstract：:The genetic compositions of 71 isoniazid-resistant Mycobacterium tuberculosis strains from Poland were determined by spoligotyping. Nearly 80% of the isolates belonged to either the T or the Haarlem family. The genotypic diversity was largely due to variation within those families. The scarcity of imported genotypes s...

journal_title：Journal of clinical microbiology

authors： Augustynowicz-Kopec E,Jagielski T,Zwolska Z

更新日期：2008-12-01 00:00:00

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:Whole-cell chromosomal digests of 54 isolates of Streptococcus suis encompassing all known serotypes from a geographically varied collection were examined by PstI restriction fragment length polymorphisms and then hybridized with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from a mixture of 16S and 23S rRNAs ...

journal_title：Journal of clinical microbiology

authors： Okwumabua O,Staats J,Chengappa MM

更新日期：1995-04-01 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...

journal_title：Journal of clinical microbiology

authors： Takizawa Y,Taneike I,Nakagawa S,Oishi T,Nitahara Y,Iwakura N,Ozaki K,Takano M,Nakayama T,Yamamoto T

更新日期：2005-07-01 00:00:00

abstract：:The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens....

journal_title：Journal of clinical microbiology

authors： Rudd RJ,Trimarchi CV

更新日期：1987-08-01 00:00:00

abstract：:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

journal_title：Journal of clinical microbiology

authors： Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

更新日期：2014-12-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ,Fenwick BW

更新日期：2021-01-21 00:00:00