Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping).

abstract：:A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specifici...

journal_title：Journal of clinical microbiology

authors： Kok T,Barancek K,Burrell CJ

更新日期：1990-06-01 00:00:00

abstract：:A group of 120 sera from blood donors was screened by complement fixation and commercially available immunofluorescence, solid-phase fluorescence immunoassay, enzyme-linked immunosorbent assay, and indirect hemagglutination tests. Twenty-four of the sera were positive by three or more of the five tests and judged to b...

journal_title：Journal of clinical microbiology

authors： Phipps PH,Grégoire L,Rossier E,Perry E

更新日期：1983-12-01 00:00:00

abstract：:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses. ...

journal_title：Journal of clinical microbiology

authors： Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli M

更新日期：2001-05-01 00:00:00

abstract：:While foreign-born persons constitute only 11% of the population in the state of Rhode Island, they account for more than 65% of incident tuberculosis (TB) annually. We investigated the molecular-epidemiological differences between foreign-born and U.S.-born TB patients to estimate the degree of recent transmission an...

journal_title：Journal of clinical microbiology

authors： Vanhomwegen J,Kwara A,Martin M,Gillani FS,Fontanet A,Mutungi P,Crellin J,Obaro S,Gosciminski M,Carter EJ,Rastogi N

更新日期：2011-03-01 00:00:00

abstract：:Helicobacter pylori is a gram-negative, curved, rod-shaped bacterium known to cause gastritis and to be an important factor in the pathogenesis of peptic ulcers. Serological testing has recently been proposed as an aid in diagnosis of H. pylori infections. In this study, we used the Western immunoblot technique to eva...

journal_title：Journal of clinical microbiology

authors： Andersen LP,Espersen F

更新日期：1992-07-01 00:00:00

abstract：:We studied 315 coagulase-negative Staphylococcus strains recovered prospectively during 240 surgical procedures (206 subjects) from proven or suspected device-associated bone and joint infections. Sixteen strains (5.1%) had decreased susceptibility to glycopeptides: 15 (12 S. epidermidis strains, 2 S. capitis strains,...

journal_title：Journal of clinical microbiology

authors： Cremniter J,Slassi A,Quincampoix JC,Sivadon-Tardy V,Bauer T,Porcher R,Lortat-Jacob A,Piriou P,Judet T,Herrmann JL,Gaillard JL,Rottman M

更新日期：2010-04-01 00:00:00

abstract：:Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enteroto...

journal_title：Journal of clinical microbiology

authors： Gustafsson B,Möllby R

更新日期：1982-02-01 00:00:00

abstract：:From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-...

journal_title：Journal of clinical microbiology

authors： Bonnet R,Souweine B,Gauthier G,Rich C,Livrelli V,Sirot J,Joly B,Forestier C

更新日期：1998-06-01 00:00:00

abstract：:The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...

journal_title：Journal of clinical microbiology

authors： Zaia JA,Gallez-Hawkins G,Churchill MA,Morton-Blackshere A,Pande H,Adler SP,Schmidt GM,Forman SJ

更新日期：1990-12-01 00:00:00

abstract：:The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resu...

journal_title：Journal of clinical microbiology

authors： Vatanshenassan M,Boekhout T,Lass-Flörl C,Lackner M,Schubert S,Kostrzewa M,Sparbier K

更新日期：2018-08-27 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that d...

journal_title：Journal of clinical microbiology

authors： Pisanic N,Randad PR,Kruczynski K,Manabe YC,Thomas DL,Pekosz A,Klein SL,Betenbaugh MJ,Clarke WA,Laeyendecker O,Caturegli PP,Larman HB,Detrick B,Fairley JK,Sherman AC,Rouphael N,Edupuganti S,Granger DA,Granger SW,Coll

更新日期：2020-12-17 00:00:00

abstract：:In a multicenter cooperative study, an enzyme immunoassay (EIA) using purified antigen of lymphadenopathy-associated virus was compared with radioimmune precipitation (RIP) for detection of antibody to human immunodeficiency virus (HIV) in 634 patients with acquired immunodeficiency syndrome or related conditions, 687...

journal_title：Journal of clinical microbiology

authors： Handsfield HH,Wandell M,Goldstein L,Shriver K

更新日期：1987-05-01 00:00:00

abstract：:Strains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the a...

journal_title：Journal of clinical microbiology

authors： Ribeiro SC,Gomes LL,Amaral EP,Andrade MR,Almeida FM,Rezende AL,Lanes VR,Carvalho EC,Suffys PN,Mokrousov I,Lasunskaia EB

更新日期：2014-07-01 00:00:00

abstract：:Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80%...

journal_title：Journal of clinical microbiology

authors： Romero EC,Billerbeck AE,Lando VS,Camargo ED,Souza CC,Yasuda PH

更新日期：1998-05-01 00:00:00

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...

journal_title：Journal of clinical microbiology

authors： Sun Y,Shah KV,Müller M,Muñoz N,Bosch XF,Viscidi RP

更新日期：1994-09-01 00:00:00

abstract：:The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desor...

journal_title：Journal of clinical microbiology

authors： Triest D,Stubbe D,De Cremer K,Piérard D,Normand AC,Piarroux R,Detandt M,Hendrickx M

更新日期：2015-02-01 00:00:00

abstract：:RNA of the newly identified human metapneumovirus (HMPV) was detected in nasopharyngeal aspirates of 11 of 63 (17.5%) young children with respiratory tract disease. Markers of infection caused by another member of the Pneumovirinae subfamily of the family Paramyxoviridae, respiratory syncytial virus (RSV), were identi...

journal_title：Journal of clinical microbiology

authors： Viazov S,Ratjen F,Scheidhauer R,Fiedler M,Roggendorf M

更新日期：2003-07-01 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:Blood culture bottles with antimicrobial removal systems are recommended for patients who develop fever while on antibiotics. This study compared the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Alert FA bottles to effectively remove vancomycin, cefoxitin, ceftriaxone, ...

journal_title：Journal of clinical microbiology

authors： Flayhart D,Borek AP,Wakefield T,Dick J,Carroll KC

更新日期：2007-03-01 00:00:00

abstract：:This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA ins...

journal_title：Journal of clinical microbiology

authors： Iversen J,Stendal G,Gerdes CM,Meyer CH,Andersen CØ,Frimodt-Møller N

更新日期：2016-02-01 00:00:00

abstract：:The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on ...

journal_title：Journal of clinical microbiology

authors： Hofmann-Thiel S,Molodtsov N,Antonenka U,Hoffmann H

更新日期：2016-12-01 00:00:00

abstract：:Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isol...

journal_title：Journal of clinical microbiology

authors： Shafer RW,Winters MA,Mayers DL,Japour AJ,Kuritzkes DR,Weislow OS,White F,Erice A,Sannerud KJ,Iversen A,Pena F,Dimitrov D,Frenkel LM,Reichelderfer PS

更新日期：1996-07-01 00:00:00

abstract：:Over a 6-year period, 24 extended-spectrum beta-lactamase (ESBL)-producing isolates of Pseudomonas aeruginosa were collected from 18 patients living in a nursing home. These isolates had a delayed development of a red pigment and exhibited a similar antibiotype (resistance to all beta-lactams except for imipenem and t...

journal_title：Journal of clinical microbiology

authors： Dubois V,Arpin C,Noury P,Andre C,Coulange L,Quentin C

更新日期：2005-08-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage typ...

journal_title：Journal of clinical microbiology

authors： Thong KL,Goh YL,Yasin RM,Lau MG,Passey M,Winston G,Yoannes M,Pang T,Reeder JC

更新日期：2002-11-01 00:00:00

abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

journal_title：Journal of clinical microbiology

authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

更新日期：1981-02-01 00:00:00

abstract：:A selective medium (CFAT) was developed for the detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. Neutral acriflavin and potassium tellurite were used in combination with the known selective agents cadmium and fluoride to eliminate most of the competing plaque flora. Compos...

journal_title：Journal of clinical microbiology

authors： Zylber LJ,Jordan HV

更新日期：1982-02-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00

abstract：:The Tigecycline Evaluation and Surveillance Trial (TEST) was designed to monitor global longitudinal changes in bacterial susceptibility to a panel of antimicrobial agents, including tigecycline. In this study, we examine susceptibility among Gram-positive isolates collected from pediatric patients globally between 20...

journal_title：Journal of clinical microbiology

authors： Brandon M,Dowzicky MJ

更新日期：2013-07-01 00:00:00