Evaluation of the Abbott RealTime MTB and RealTime MTB INH/RIF Assays for Direct Detection of Mycobacterium tuberculosis Complex and Resistance Markers in Respiratory and Extrapulmonary Specimens.

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

journal_title：Journal of clinical microbiology

authors： Niimi K,Shepherd MG,Cannon RD

更新日期：2001-06-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott mPLUS featur...

journal_title：Journal of clinical microbiology

authors： Lucic D,Jones S,Wiesneth R,Barry C,Webb E,Belova L,Dolan P,Ho S,Abravaya K,Cloherty G

更新日期：2013-12-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

journal_title：Journal of clinical microbiology

authors： Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

更新日期：1996-01-01 00:00:00

abstract：:During a survey of Group G and C streptococcal infections of humans two epidemiologically unrelated Group G streptococcal isolates were identified, one from a case of bacteremia and one from a wound infection. These isolates were atypical among this sample in that the emm gene could not be amplified from them by PCR. ...

journal_title：Journal of clinical microbiology

authors： Whatmore AM,Engler KH,Gudmundsdottir G,Efstratiou A

更新日期：2001-11-01 00:00:00

abstract：:We report the first case of maxillary sinusitis caused by Actinomucor elegans in an 11-year-old patient. Histopathological and mycological examinations of surgical maxillary sinuses samples showed coenocytic hyphae characteristic of mucoraceous fungi. The fungi recovered had stolons and rhizoids, nonapophyseal and glo...

journal_title：Journal of clinical microbiology

authors： Davel G,Featherston P,Fernández A,Abrantes R,Canteros C,Rodero L,Sztern C,Perrotta D

更新日期：2001-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

journal_title：Journal of clinical microbiology

authors： Fridriksdóttir V,Nesse LL,Gudding R

更新日期：1992-05-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...

journal_title：Journal of clinical microbiology

authors： Spilker T,Baldwin A,Bumford A,Dowson CG,Mahenthiralingam E,LiPuma JJ

更新日期：2009-08-01 00:00:00

abstract：:Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...

journal_title：Journal of clinical microbiology

authors： Steingrube VA,Brown BA,Gibson JL,Wilson RW,Brown J,Blacklock Z,Jost K,Locke S,Ulrich RF,Wallace RJ Jr

更新日期：1995-12-01 00:00:00

abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

journal_title：Journal of clinical microbiology

authors： Kotilainen P

更新日期：1990-12-01 00:00:00

abstract：:Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necro...

journal_title：Journal of clinical microbiology

authors： Van Bost S,Jacquemin E,Oswald E,Mainil J

更新日期：2003-09-01 00:00:00

abstract：:Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

journal_title：Journal of clinical microbiology

authors： Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

更新日期：1999-12-01 00:00:00

abstract：:Human (n = 33) and canine (n = 53) Campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. We observed 100% typeability and high overall diversity. The majority of human strains (23/33) clustered separately from canine strains, indicating that dogs...

journal_title：Journal of clinical microbiology

authors： Damborg P,Guardabassi L,Pedersen K,Kokotovic B

更新日期：2008-04-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and ...

journal_title：Journal of clinical microbiology

authors： Zhang G,Weintraub A

更新日期：1998-12-01 00:00:00

abstract：:The diagnosis and management of pneumonia are limited by the use of culture-based techniques of microbial identification, which may fail to identify unculturable, fastidious, and metabolically active viable but unculturable bacteria. Novel high-throughput culture-independent techniques hold promise but have not been s...

journal_title：Journal of clinical microbiology

authors： Dickson RP,Erb-Downward JR,Prescott HC,Martinez FJ,Curtis JL,Lama VN,Huffnagle GB

更新日期：2014-10-01 00:00:00

abstract：:From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and a...

journal_title：Journal of clinical microbiology

authors： Tran A,Talmud D,Lejeune B,Jovenin N,Renois F,Payan C,Leveque N,Andreoletti L

更新日期：2010-05-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

journal_title：Journal of clinical microbiology

authors： Nimri LF

更新日期：1993-10-01 00:00:00

abstract：:A total of 1,013 isolates of Enterobacteriaceae were identified in parallel by the Repliscan (Cathra International, Ontario, Canada) and API 20E (Analytab Products, Plainview, N.Y.) systems. There was a 62% agreement at the genus level between the two systems. Of the 38% discrepant results, Repliscan classified 22% as...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Quall CO,Fox JM

更新日期：1981-01-01 00:00:00

abstract：:Eleven isolates representing five distinct outbreaks of pneumococcal conjunctivitis were examined for phenotypic and genetic characteristics. None of the strains possessed capsules, and all strains were susceptible to optochin, bile soluble, and Gen-Probe AccuProbe test positive. All 11 isolates were confirmed as Stre...

journal_title：Journal of clinical microbiology

authors： Carvalho MG,Steigerwalt AG,Thompson T,Jackson D,Facklam RR

更新日期：2003-09-01 00:00:00

abstract：:The AutoSCAN-3 (American MicroScan, Mahwah, N.J.) is an instrument capable of automated reading of commercially available microdilution trays for identification and quantitative susceptibility testing of rapidly growing bacteria. This study compared the results of visual and automated reading of microdilution trays fo...

journal_title：Journal of clinical microbiology

authors： DeGirolami PC,Eichelberger KA,Salfity LC,Rizzo MF

更新日期：1983-12-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...

journal_title：Journal of clinical microbiology

authors： Bai GH,Kim SJ,Chang CL,National and Regional Tuberculosis Reference Laboratories.

更新日期：2007-11-01 00:00:00

abstract：:Endotoxin concentrations were determined in middle-ear effusions (MEEs) from 89 children with chronic otitis media by using the Limulu's amoebocyte lysate assay. Mean concentrations of endotoxin in Haemophilus influenzae-positive and Streptococcus pneumoniae-positive MEEs were 157 and 21.8 ng/ml, respectively, and wer...

journal_title：Journal of clinical microbiology

authors： DeMaria TF,Prior RB,Briggs BR,Lim DJ,Birck HG

更新日期：1984-07-01 00:00:00