Abstract:
:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli Mdoi
10.1128/JCM.39.5.2022-2024.2001subject
Has Abstractpub_date
2001-05-01 00:00:00pages
2022-4issue
5eissn
0095-1137issn
1098-660Xjournal_volume
39pub_type
杂志文章abstract::A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.10.3094-3095.1998
更新日期:1998-10-01 00:00:00
abstract::A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conse...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00542-06
更新日期:2006-08-01 00:00:00
abstract::The utility of the GeneXpert MTB/RIF (Xpert) assay for detection of Mycobacterium tuberculosis in sputum samples has been extensively studied. However, the performance of the Xpert assay as applied to other readily accessible body fluids such as exhaled breath condensate (EBC), saliva, urine, and blood has not been es...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01743-13
更新日期:2013-12-01 00:00:00
abstract::Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.3.1112-1117.2005
更新日期:2005-03-01 00:00:00
abstract::We report five new IS711 chromosomal locations that are specific for marine mammal Brucella groups of strains and useful for their identification and classification. Our data support their current classification into two species, Brucella ceti and B. pinnipedialis, with subgroups in each, but also the possibility of a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01069-10
更新日期:2010-10-01 00:00:00
abstract::Real-time PCR shows the widespread presence of Coxiella burnetii DNA in a broad range of commercially available milk and milk products. MLVA genotyping shows that this is the result of the presence of a predominant C. burnetii genotype in the dairy cattle population. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.06831-11
更新日期:2012-06-01 00:00:00
abstract::Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::E test strips (AB Biodisk, Culver City, Calif.) tested on 2% NaCl-supplemented Mueller-Hinton agar and Alamar panels (Alamar, Sacramento, Calif.) correctly characterized 127 of 127 methicillin-resistant Staphylococcus aureus isolates and 100 of 100 methicillin-susceptible S. aureus isolates. These overnight antimicrob...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.11.3056-3057.1993
更新日期:1993-11-01 00:00:00
abstract::In order to investigate the seroprevalence of human herpesvirus 8 (HHV-8) infection in central and southern Italy, sera from human immunodeficiency virus (HIV)-seronegative subjects, with and without Kaposi's sarcoma (KS), were analyzed by immunofluorescence assay, using BC-3, a cell line latently infected with HHV-8....
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.4.1150-1153.1999
更新日期:1999-04-01 00:00:00
abstract::Antibiotic susceptibility studies were compared by the quantitative minimum inhibitory concentration and Autobac I. Four hundred common isolates from clinical materials including both gram-negative and -positive organisms were evaluated. Results demonstrate that the Autobac I method can provide reliable semiquantitati...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.11.5.465-469.1980
更新日期:1980-05-01 00:00:00
abstract::Norovirus (NV) is the most common causative agent of nonbacterial gastroenteritis. Reports of surveillance of NV in facilities that reported outbreaks are frequently found in publications, but reports of that in facilities without outbreaks are not found. We investigated the molecular epidemiology of NV isolates deriv...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00305-08
更新日期:2008-06-01 00:00:00
abstract::Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.14.6.684-685.1981
更新日期:1981-12-01 00:00:00
abstract::A total of 137 strains of Staphylococcus species were blindly tested by four rapid serological assays, and the results were compared with those of the tube coagulase assay. For the S. aureus isolates, the Sero-STAT Staph assay gave six false-negative results, four of which were for methicillin-resistant strains. The A...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.5.703-704.1984
更新日期:1984-05-01 00:00:00
abstract::Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,meta分析
doi:10.1128/JCM.02057-18
更新日期:2019-05-24 00:00:00
abstract::PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was applied to 108 mycobacterial isolates representing 34 species to evaluate its potential as a rapid reference method. A total...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.11.2969-2973.1997
更新日期:1997-11-01 00:00:00
abstract::Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.32.9.2216-2220.1994
更新日期:1994-09-01 00:00:00
abstract::Subgroup J avian leukosis virus (ALV-J) is an avian retrovirus that causes severe economic losses in the poultry industry. The early identification and removal of virus-shedding birds are important to reduce the spread of congenital and contact infections. In this study, a TaqMan-based real-time PCR method for the rap...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02030-12
更新日期:2013-01-01 00:00:00
abstract::The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.8.1411-1415.1987
更新日期:1987-08-01 00:00:00
abstract::Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.1.315-322.2001
更新日期:2001-01-01 00:00:00
abstract::Crude extracts of Dolichos biflorus can be coupled to polystyrene particles to yield an agglutination reagent for the detection of group C streptococcal antigen extracts. The reagent is relatively inexpensive and simple to prepare and can be employed for the definitive identification of this beta-hemolytic streptococc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.1.83-84.1984
更新日期:1984-01-01 00:00:00
abstract::Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.6.2816-2818.2004
更新日期:2004-06-01 00:00:00
abstract::A cross-sectional study of nasopharyngeal colonization with Streptococcus pneumoniae was performed among 573 children attending 29 day-care centers (DCCs) in Norway prior to the start of mass vaccination with the heptavalent pneumococcal conjugate vaccine (PCV-7). A sensitive sampling method was employed, including tr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02296-07
更新日期:2008-08-01 00:00:00
abstract::Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.8.1793-1796.1989
更新日期:1989-08-01 00:00:00
abstract::The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.1.199-201.1999
更新日期:1999-01-01 00:00:00
abstract::A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.8.1464-1470.1988
更新日期:1988-08-01 00:00:00
abstract::Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01261-19
更新日期:2019-11-22 00:00:00
abstract::Microfiltration has become a popular procedure for the concentration and enumeration of bacteria. We developed a rapid and sensitive method for the differentiation of gram-positive and gram-negative bacteria, utilizing a polycarbonate membrane filter, crystal violet, iodine, 95% ethanol, and 6% carbol fuchsin, that ca...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.7.1378-1382.1988
更新日期:1988-07-01 00:00:00
abstract::A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.5.852-854.1984
更新日期:1984-11-01 00:00:00
abstract::Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00090-09
更新日期:2009-06-01 00:00:00