Abstract:
:Microfiltration has become a popular procedure for the concentration and enumeration of bacteria. We developed a rapid and sensitive method for the differentiation of gram-positive and gram-negative bacteria, utilizing a polycarbonate membrane filter, crystal violet, iodine, 95% ethanol, and 6% carbol fuchsin, that can be completed in 60 to 90 s. Gram reactions of 49 species belonging to 30 genera of bacteria were correctly determined by the filter-Gram stain. The sensitivities of the filter-Gram stain and conventional slide-Gram stain were compared by testing dilutions of Escherichia coli, Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae suspensions in the presence and absence of whole human blood. The filter-Gram stain was approximately 100-fold more sensitive than the slide-Gram stain. The filter-Gram stain detected 2 to 100 bacteria, whereas the slide-Gram stain failed to detect less than 1,000 bacteria. The sensitivities of the methods were not significantly altered by the addition of whole human blood to the dilutions of bacteria tested. The filter-Gram stain could be a useful tool for the examination of body fluids with very low numbers of bacteria.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Romero S,Schell RF,Pennell DRdoi
10.1128/JCM.26.7.1378-1382.1988subject
Has Abstractpub_date
1988-07-01 00:00:00pages
1378-82issue
7eissn
0095-1137issn
1098-660Xjournal_volume
26pub_type
杂志文章abstract::We developed a strategy to determine the clinical impact associated with errors in clinical microbiology testing. Over a 9-month period, we used a sequential three-stage method to prospectively evaluate 480 consecutive corrected microbiology laboratory reports. The three stages were physician review of the corrected r...
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journal_title:Journal of clinical microbiology
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pub_type: 杂志文章
doi:10.1128/JCM.26.12.2572-2575.1988
更新日期:1988-12-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.2.409-413.1998
更新日期:1998-02-01 00:00:00
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doi:10.1128/JCM.20.3.586-.1984
更新日期:1984-09-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.39.12.4264-4268.2001
更新日期:2001-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00120-10
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doi:10.1128/JCM.37.7.2343-2345.1999
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.2.201-205.1988
更新日期:1988-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.5.944-948.1990
更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.9.2491-2494.1998
更新日期:1998-09-01 00:00:00
abstract::A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Journal of clinical microbiology
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