DNA macroarray for identification and typing of Staphylococcus aureus isolates.

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:We describe the adoption of nucleic acid amplification tests (NAAT) for Clostridium difficile diagnosis and their impact on stool rejection policies and C. difficile positivity rates. Of the laboratories with complete surveys, 51 (43%) reported using NAAT in 2011. Laboratories using NAAT had stricter rejection policie...

journal_title：Journal of clinical microbiology

authors： Cohen J,Limbago B,Dumyati G,Holzbauer S,Johnston H,Perlmutter R,Dunn J,Nadle J,Lyons C,Phipps E,Beldavs Z,Clark LA,Lessa FC,CDC's Clostridium difficile Infection Surveillance Investigators.

更新日期：2014-02-01 00:00:00

abstract：:We examined Enterococcus faecalis strains clinically isolated from 100 patients with urinary tract infections (UTIs) for their susceptibility to levofloxacin (LVX) by measuring the MIC and investigated amino acid mutations by direct DNA sequencing, which were then correlated with LVX resistance. Next, we studied risk ...

journal_title：Journal of clinical microbiology

authors： Yasufuku T,Shigemura K,Shirakawa T,Matsumoto M,Nakano Y,Tanaka K,Arakawa S,Kawabata M,Fujisawa M

更新日期：2011-11-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced by clinical isolates of enterotoxigenic E. coli (STa-ETEC) by a single monoclonal antibody-based inhibition GM1 enzyme-li...

journal_title：Journal of clinical microbiology

authors： Sanchez J,Holmgren J,Svennerholm AM

更新日期：1990-10-01 00:00:00

abstract：:We have developed a new research assay that combines sequence-specific sample preparation and isothermal amplification for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae infections. The assay targets both the omp gene and the cryptic plasmid of C. trachomatis and the multicopy opa gene of N. gonorrho...

journal_title：Journal of clinical microbiology

authors： O'Neil D,Doseeva V,Rothmann T,Wolff J,Nazarenko I

更新日期：2011-12-01 00:00:00

abstract：:Ten clinical yeast isolates submitted to the Centers for Disease Control from diverse geographic areas were identified as Candida ciferrii and Candida chiropterorum. The association of C. ciferrii with clinical specimens, particularly its repeated isolation from a case of onychomycosis, suggests that this species may ...

journal_title：Journal of clinical microbiology

authors： Furman RM,Ahearn DG

更新日期：1983-11-01 00:00:00

abstract：:Seventy-six nontuberculous mycobacterial isolates obtained from patients living in Greece were analyzed with the GenoType Mycobacterium CM (for common mycobacteria) and AS (for additional species) assays. GenoType correctly identified all but one of the mycobacterial species. For this species, additional probes should...

journal_title：Journal of clinical microbiology

authors： Gitti Z,Neonakis I,Fanti G,Kontos F,Maraki S,Tselentis Y

更新日期：2006-06-01 00:00:00

abstract：:Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

journal_title：Journal of clinical microbiology

authors： Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

更新日期：2006-09-01 00:00:00

abstract：:Polymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this i...

journal_title：Journal of clinical microbiology

authors： Satlin MJ

更新日期：2019-01-30 00:00:00

abstract：:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

journal_title：Journal of clinical microbiology

authors： Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

更新日期：2007-08-01 00:00:00

abstract：:A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells sim...

journal_title：Journal of clinical microbiology

authors： Siitari H,Turunen P,Schrimsher J,Nunn M

更新日期：1990-09-01 00:00:00

abstract：:In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using...

journal_title：Journal of clinical microbiology

authors： Aidoo S,Ampofo WK,Brandful JA,Nuvor SV,Ansah JK,Nii-Trebi N,Barnor JS,Apeagyei F,Sata T,Ofori-Adjei D,Ishikawa K

更新日期：2001-07-01 00:00:00

abstract：:Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

journal_title：Journal of clinical microbiology

authors： Carlson JR,McCarthy LR

更新日期：1979-03-01 00:00:00

abstract：:Monoclonal antibodies were generated from mice immunized with scolex protein antigen of Cysticercus cellulosae. Three monoclonal antibodies specific for cysticercal antigens, which did not show any cross-reactivity with Taenia solium or Taenia saginata antigens, were selected. Each monoclonal antibody coupled to Sepha...

journal_title：Journal of clinical microbiology

authors： Nascimento E,Tavares CA,Lopes JD

更新日期：1987-07-01 00:00:00

abstract：:The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

journal_title：Journal of clinical microbiology

authors： Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

更新日期：2010-06-01 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Callegaro A,Scarparo C,Penati V,Nista D,Bornigia S,Lacchini C,Scagnelli M,Santini G,De Sio G

更新日期：1998-12-01 00:00:00

abstract：:BBL CHROMagar VanRE (CVRE) was compared with bile esculin azide agar plus vancomycin to screen for vancomycin-resistant enterococcus (VRE) colonization. CVRE distinguishes Enterococcus faecalis (green colonies) from Enterococcus faecium (mauve colonies) on the basis of chromogenic substrate use. CVRE sensitivity and s...

journal_title：Journal of clinical microbiology

authors： Kallstrom G,Doern CD,Dunne WM Jr

更新日期：2010-03-01 00:00:00

abstract：:A combined clinical and molecular epidemiologic analysis of 46 strains of Clostridium difficile, including 16 nosocomial isolates from one ward (outbreak ward) plus 17 other nosocomial isolates and 13 community-acquired isolates, was performed. HindIII digests of total cellular DNA were analyzed by restriction enzyme ...

journal_title：Journal of clinical microbiology

authors： Kristjánsson M,Samore MH,Gerding DN,DeGirolami PC,Bettin KM,Karchmer AW,Arbeit RD

更新日期：1994-08-01 00:00:00

abstract：:In Mycobacterium smegmatis and a limited number of Mycobacterium tuberculosis strains, the involvement of alterations of the 16S rRNA gene (rrs) in resistance to kanamycin has been shown. To investigate the extent to which mutations in a specific region of the rrs gene and the kanamycin-resistant phenotype in clinical...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Katsukawa C,Tamaru A,Abe C,Makino M,Mizuguchi Y,Taniguchi H

更新日期：1998-05-01 00:00:00

abstract：:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses. ...

journal_title：Journal of clinical microbiology

authors： Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli M

更新日期：2001-05-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

journal_title：Journal of clinical microbiology

authors： Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

更新日期：2000-06-01 00:00:00

abstract：:Herpes simplex virus type 2 (HSV-2) seroprevalence in 68 patients with AIDS was 77% by Western blot (immunoblot) and 44% by glycoprotein G-2 immunoassay. Each of 16 patients with culture-proven HSV-2 infection was positive by Western blot versus 8 by glycoprotein G-2 immunoassay. No differences in age, race, duration ...

journal_title：Journal of clinical microbiology

authors： Safrin S,Arvin A,Mills J,Ashley R

更新日期：1992-05-01 00:00:00

abstract：:Growth on different media and the influence of culture conditions were studied on 19 recently isolated strains of Haemophilus ducreyi, none of which had more than four passages on artificial media. The results were compared with 10 laboratory strains, which had an unknown number of passages in vitro. For all strains, ...

journal_title：Journal of clinical microbiology

authors： Sturm AW,Zanen HC

更新日期：1984-05-01 00:00:00

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:It is unclear whether patients colonized with methicillin-resistant Staphylococcus aureus (MRSA) continue to harbor the same genotype during hospital readmissions. We characterized 140 MRSA strains isolated from 33 persistent MRSA carriers with hospital readmissions. Nearly half of the patients continued to harbor the...

journal_title：Journal of clinical microbiology

authors： Eghbaria S,Schnitser K,Danial-Farran N,Keness Y,Bisharat N

更新日期：2014-04-01 00:00:00