Dichotomy of glycoprotein g gene in herpes simplex virus type 1 isolates.

Abstract:

:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-specific monoclonal antibody (MAb) to screen for mutations in the immunodominant region of this protein in 108 clinical HSV-1 isolates. Of these, 42 isolates showed no reactivity to the anti-gG-1 MAb. One hundred five strains were further examined by DNA sequencing of the middle part of the gG-1 gene, encompassing 106 amino acids including the immunodominant region and epitope of the anti-gG-1 MAb. By phylogenetic comparisons based on the sequence data, we observed two (main) genetic variants of the gG-1 gene among the clinical isolates corresponding to reactivity or nonreactivity to the anti-gG-1 MAb. Furthermore, four strains appeared to be recombinants of the two gG-1 variants. In addition, one strain displayed a gG-1-negative phenotype due to a frameshift mutation, in the form of insertion of a cytosine nucleotide. When immunoglobulin G reactivity to HSV-1 in sera from patients infected with either of the two variants was investigated, no significant differences were found between the two groups, either in a type-common enzyme-linked immunosorbent assay (ELISA) or in a type-specific gG-1 antigen-based ELISA. Despite the here-documented existence of two variants of the gG-1 gene affecting the immunodominant region of the protein, other circumstances, such as early phase of infection, might be sought for explaining the seronegativity to gG-1 commonly found in a proportion of the HSV-1-infected patients.

journal_name

J Clin Microbiol

authors

Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

doi

10.1128/jcm.40.9.3245-3251.2002

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

3245-51

issue

9

eissn

0095-1137

issn

1098-660X

journal_volume

40

pub_type

杂志文章
  • Erroneous reporting of coagulase-negative Staphylococci as Kocuria spp. by the Vitek 2 system.

    abstract::Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isola...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.3.1448-1450.2005

    authors: Ben-Ami R,Navon-Venezia S,Schwartz D,Schlezinger Y,Mekuzas Y,Carmeli Y

    更新日期:2005-03-01 00:00:00

  • Cavitating pneumonia caused by Corynebacterium group JK.

    abstract::Corynebacterium group JK organisms were isolated from lung fluid aspirated with a fine needle from one of four thick-walled cavitary lesions in a 72-year-old female. Cultures and stains for mycobacteria and fungi were negative. The patient was successfully treated with an 8-week course of vancomycin. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.10.2216-2217.1988

    authors: McNaughton RD,Villanueva RR,Donnelly R,Freedman J,Nawrot R

    更新日期:1988-10-01 00:00:00

  • Clinical implications of evolutionary patterns of homologous, full-length hepatitis B virus quasispecies in different hosts after perinatal infection.

    abstract::Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03338-13

    authors: Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

    更新日期:2014-05-01 00:00:00

  • Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures.

    abstract::The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00485-18

    authors: Granato PA,Unz MM,Widen RH,Silbert S,Young S,Heflin KL,Conover MS,Buchan BW,Ledeboer NA

    更新日期:2018-08-27 00:00:00

  • Recovery of Salmonella group B from blood and Salmonella group C2 from feces and serological evidence of dual infection in one patient.

    abstract::A patient with a dual Salmonella infection is described. Salmonella group B was recovered from three blood culture sets but was not detected in seven stool cultures. Salmonella group C2 was isolated from three of seven stool cultures but was not recovered from blood cultures. Specific, non-cross-reactive antibodies to...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.3.584-585.1984

    authors: Papasian CJ,Bartholomew WR,Neter E,Amsterdam D

    更新日期:1984-09-01 00:00:00

  • Characterization and distribution of Pasteurella species recovered from infected humans.

    abstract::During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.11.2984-2987.1992

    authors: Holst E,Rollof J,Larsson L,Nielsen JP

    更新日期:1992-11-01 00:00:00

  • Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation.

    abstract::A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.8.3827-3830.2004

    authors: Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

    更新日期:2004-08-01 00:00:00

  • Genital Candida species detected in samples from women in Melbourne, Australia, before and after treatment with antibiotics.

    abstract::Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00218-06

    authors: Pirotta MV,Garland SM

    更新日期:2006-09-01 00:00:00

  • Tear fluid of hepatitis C virus carriers could be infectious.

    abstract::For up to 20 to 40% of patients chronically infected with hepatitis C virus (HCV), the mode of transmission is still unknown. We demonstrate that tear fluid contains HCV RNA-carrying material with the properties of infectious virus and conclude that smear infection with tear fluid may play a role in HCV transmission. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.8.2202-2203.1995

    authors: Feucht HH,Zöllner B,Schröter M,Altrogge H,Laufs R

    更新日期:1995-08-01 00:00:00

  • An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species.

    abstract::An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH pr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.1.92-97.1994

    authors: Switchenko AC,Miyada CG,Goodman TC,Walsh TJ,Wong B,Becker MJ,Ullman EF

    更新日期:1994-01-01 00:00:00

  • Genome sequencing of Mycobacterium abscessus isolates from patients in the united states and comparisons to globally diverse clinical strains.

    abstract::Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01144-14

    authors: Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

    更新日期:2014-10-01 00:00:00

  • Selective buffered charcoal-yeast extract medium for isolation of nocardiae from mixed cultures.

    abstract::The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.7.1891-1892.1992

    authors: Garrett MA,Holmes HT,Nolte FS

    更新日期:1992-07-01 00:00:00

  • Prospective study of Campylobacter jejuni infection in Chilean infants evaluated by culture and serology.

    abstract::A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.5.1040-1044.1989

    authors: Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

    更新日期:1989-05-01 00:00:00

  • Isolation of an unusual mycobacterium from an AIDS patient.

    abstract::A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.9.2316-2319.1996

    authors: Tortoli E,Kirschner P,Bartoloni A,Burrini C,Manfrin V,Mantella A,Scagnelli M,Scarparo C,Simonetti MT,Böttger EC

    更新日期:1996-09-01 00:00:00

  • Luminol-amplified chemiluminescence: a sensitive method for detecting the carrier state in chronic granulomatous disease.

    abstract::Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.1.52-56.1980

    authors: Mills EL,Rholl KS,Quie PG

    更新日期:1980-07-01 00:00:00

  • Indirect enzyme-linked immunosorbent assay for detection of Brucella melitensis-specific antibodies in goat milk.

    abstract::Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.2.721-725.2005

    authors: Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

    更新日期:2005-02-01 00:00:00

  • Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens.

    abstract::A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00728-17

    authors: Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

    更新日期:2017-12-01 00:00:00

  • Interlaboratory comparison of the toluidine red unheated serum test antigen preparation.

    abstract::The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.3.434-437.1984

    authors: Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

    更新日期:1984-09-01 00:00:00

  • Use of Automated Urine Microscopy Analysis in Clinical Diagnosis of Urinary Tract Infection: Defining an Optimal Diagnostic Score in an Academic Medical Center Population.

    abstract::A retrospective case record study was conducted that established a scoring tool based on clinical and iQ200 parameters, able to predict or rule out the clinical diagnosis of UTI in the majority of adult patients in an academic hospital. Automated standardized quantitative urine analysis, such as iQ200 analysis, is on ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02030-17

    authors: Foudraine DE,Bauer MP,Russcher A,Kusters E,Cobbaert CM,van der Beek MT,Stalenhoef JE

    更新日期:2018-05-25 00:00:00

  • Epidemiology of Invasive Group B Streptococcal Disease in Alberta, Canada, from 2003 to 2013.

    abstract::Group B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding anti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00355-16

    authors: Alhhazmi A,Hurteau D,Tyrrell GJ

    更新日期:2016-07-01 00:00:00

  • Susceptibility testing of fungi: current status of correlation of in vitro data with clinical outcome.

    abstract::In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.34.3.489-495.1996

    authors: Ghannoum MA,Rex JH,Galgiani JN

    更新日期:1996-03-01 00:00:00

  • Rapid identification of smooth Brucella species with a monoclonal antibody.

    abstract::A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.11.2090-2093.1987

    authors: Roop RM 2nd,Preston-Moore D,Bagchi T,Schurig GG

    更新日期:1987-11-01 00:00:00

  • Recognition of a new serogroup of Legionnaires disease bacterium.

    abstract::A strain of the Legionnaires disease bacterium (LDB) that was isolated by Joseph E. McDade from a postmortem lung specimen of a patient with fatal atypical pneumonia at the Veterans Administration Hospital in Togus, Maine was serologically different from 16 other strains of LDB that had been isolated previously from p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: McKinney RM,Thomason BM,Harris PP,Thacker L,Lewallen KR,Wilkinson HW,Hebert GA,Moss CW

    更新日期:1979-01-01 00:00:00

  • Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis.

    abstract::A total of 46 Borrelia burgdorferi sensu lato isolates that were isolated from patients with Lyme borreliosis and infected animals or were extracted from ticks of the genus Ixodes were analyzed. Large restriction fragment patterns obtained after cleavage of genomic DNAs with MluI were analyzed by pulsed-field gel elec...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.11.2873-2877.1993

    authors: Belfaiza J,Postic D,Bellenger E,Baranton G,Girons IS

    更新日期:1993-11-01 00:00:00

  • PCR of peripheral blood for diagnosis of meningococcal disease.

    abstract::Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JCM.34.7.1637-1640.1996

    authors: Newcombe J,Cartwright K,Palmer WH,McFadden J

    更新日期:1996-07-01 00:00:00

  • Serratia rubidaea as an invasive pathogen.

    abstract::Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.1.216-217.1996

    authors: Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

    更新日期:1996-01-01 00:00:00

  • Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan.

    abstract::We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the inves...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01263-15

    authors: Kawahara R,Seto K,Taguchi M,Nakajima C,Kumeda Y,Suzuki Y

    更新日期:2015-09-01 00:00:00

  • Molecular characterization of endocarditis-associated Staphylococcus aureus.

    abstract::Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00651-13

    authors: Nethercott C,Mabbett AN,Totsika M,Peters P,Ortiz JC,Nimmo GR,Coombs GW,Walker MJ,Schembri MA

    更新日期:2013-07-01 00:00:00

  • Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested.

    abstract::Six rapid agglutination tests for identification of Staphylococcus aureus were evaluated by using 62 strains of S. aureus, 63 strains of S. saprophyticus, and 67 strains of other coagulase-negative staphylococci. S. saprophyticus was responsible for 19 of 26 false-positive results and 20 uninterpretable reactions. Thu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.7.1398-1399.1988

    authors: Gregson DB,Low DE,Skulnick M,Simor AE

    更新日期:1988-07-01 00:00:00

  • Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci.

    abstract::Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalid...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Fenton LJ,Harper MH

    更新日期:1979-02-01 00:00:00