Rapid identification of smooth Brucella species with a monoclonal antibody.

Abstract:

:A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal antibody with specificity for the O side chain of B. abortus. Reaction with anti-rat immunoglobulin G conjugated to horseradish peroxidase and development in 4-chloro-1-naphthol resulted in colonies of naturally occurring smooth Brucella species staining purple. Results could be obtained within 4 h after colonies were visible on plates and individual colonies could be detected. Yersinia enterocolitica serovar O:9 strains were the only other organisms tested which showed cross-reaction by using this procedure. Because of its speed, sensitivity, and specificity, this technique should be very useful for identifying smooth Brucella strains in diagnostic laboratories.

journal_name

J Clin Microbiol

authors

Roop RM 2nd,Preston-Moore D,Bagchi T,Schurig GG

doi

10.1128/JCM.25.11.2090-2093.1987

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

2090-3

issue

11

eissn

0095-1137

issn

1098-660X

journal_volume

25

pub_type

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