Abstract:
:Norovirus (NV) is the most common causative agent of nonbacterial gastroenteritis. Reports of surveillance of NV in facilities that reported outbreaks are frequently found in publications, but reports of that in facilities without outbreaks are not found. We investigated the molecular epidemiology of NV isolates derived from asymptomatic food handlers working at a non-outbreak food catering facility in Hokkaido, Japan, from February to March in 2005 and January to February in 2006 by RNA polymerase gene sequencing. Approximately 12% (20/159) of the samples were positive for genogroup II (GII; 10.1% in 2005 and 14.2% in 2006). The GI genotypes were not detected. The data from the phylogenetic analysis indicated that, among the 20 strains detected, 13 strains were GII/genotype 2 (GII/2), two were GII/3, three were GII/8, and two were GII/12. GII/4, which has been found most frequently in recent outbreaks worldwide, including Japan, was not detected. We found that one individual was coinfected with two genotypes, GII/2 and GII/12. This is the first report of the detection of NV genotypes in asymptomatic food handlers working at a non-outbreak facility. The excretion of NV from healthy individuals may be an infection source of NV outbreaks as well as other food-borne diseases.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Okabayashi T,Yokota S,Ohkoshi Y,Ohuchi H,Yoshida Y,Kikuchi M,Yano K,Fujii Ndoi
10.1128/JCM.00305-08subject
Has Abstractpub_date
2008-06-01 00:00:00pages
1985-8issue
6eissn
0095-1137issn
1098-660Xpii
JCM.00305-08journal_volume
46pub_type
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