Erroneous reporting of coagulase-negative Staphylococci as Kocuria spp. by the Vitek 2 system.

abstract：:The results of PCR-based molecular typing of Helicobacter pylori strains by restriction fragment length polymorphism analysis of a 1, 161-bp nucleotide sequence of the midregion of the vacA gene are reported. A total of 48 H. pylori strains isolated from gastric biopsy specimens obtained from 18 patients with peptic u...

journal_title：Journal of clinical microbiology

authors： Donati M,Storni E,D'Apote L,Moreno S,Tucci A,Poli L,Cevenini R

更新日期：1999-04-01 00:00:00

abstract：:An indirect fluorescent antibody (IFA) technique was evaluated as a procedure for rapid detection and identification of members of the Bacteroidaceae. Antisera were prepared against 31 members of this family, including species of Bacteroides and Fusobacterium commonly isolated from human infections. The antisera had d...

journal_title：Journal of clinical microbiology

authors： Stauffer LR,Hill EO,Holland JW,Altemeier WA

更新日期：1975-10-01 00:00:00

abstract：:The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there is currently no consensus on how "epidemic clones" should be defined. The objectives of this work were to compare the patterns of sequence diversity on two sets of ...

journal_title：Journal of clinical microbiology

authors： Cantinelli T,Chenal-Francisque V,Diancourt L,Frezal L,Leclercq A,Wirth T,Lecuit M,Brisse S

更新日期：2013-11-01 00:00:00

abstract：:A fully automated antifungal susceptibility test system recently updated to reflect the new species-specific clinical breakpoints (CBPs) of fluconazole for Candida (Vitek 2 AF03 yeast susceptibility test; bioMérieux, Inc., Durham, NC) was compared in three different laboratories with the Clinical and Laboratory Standa...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Procop GW,Wiederhold NP

更新日期：2014-06-01 00:00:00

abstract：:A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selec...

journal_title：Journal of clinical microbiology

authors： Noel JS,Lee TW,Kurtz JB,Glass RI,Monroe SS

更新日期：1995-04-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

journal_title：Journal of clinical microbiology

authors： McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

更新日期：2006-12-01 00:00:00

abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...

journal_title：Journal of clinical microbiology

authors： Meyer MP,Eddy T,Baughn RE

更新日期：1994-03-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:A total of 30 isolates of Treponema hyodysenteriae collected in the Saint-Hyacinthe (Quebec, Canada) area were serotyped by agar gel double immunodiffusion by using extracted lipopolysaccharide and hyperimmune rabbit antisera. Only 17% (5 of 30) of the isolates were typed with antisera specific for each of the seven k...

journal_title：Journal of clinical microbiology

authors： Li ZS,Bélanger M,Jacques M

更新日期：1991-12-01 00:00:00

abstract：:A survey was conducted among Spanish children with gastroenteritis treated in an emergency room. Reverse transcription-PCR with specimens negative for other enteric pathogens was used. The minimum incidence of human calicivirus infection was 7.7%, with Lordsdale as the predominant genotype. The clinical features and s...

journal_title：Journal of clinical microbiology

authors： Roman E,Negredo A,Dalton RM,Wilhelmi I,Sánchez-Fauquier A

更新日期：2002-10-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

journal_title：Journal of clinical microbiology

authors： Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

更新日期：1984-09-01 00:00:00

abstract：:Multilocus sequence typing was successfully completed on 494 isolates of Streptococcus uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12-month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis cases that occurred in different cows within the same herd an...

journal_title：Journal of clinical microbiology

authors： Davies PL,Leigh JA,Bradley AJ,Archer SC,Emes RD,Green MJ

更新日期：2016-01-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed to study the subclass distribution of immunoglobulin G (IgG) specific to the core glycolipid (CGL) of the Re mutant of Salmonella minnesota R595 in serum samples from individuals with an IgG response toward these antigens. In a group of healthy blood donors, we detect...

journal_title：Journal of clinical microbiology

authors： Nys M,Joassin L,Somzee A,Demonty J

更新日期：1988-05-01 00:00:00

abstract：:Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...

journal_title：Journal of clinical microbiology

authors： Hess E,Renzi F,Koudad D,Dol M,Cornelis GR

更新日期：2017-06-01 00:00:00

abstract：:We describe two cases of Clostridium glycolicum wound infections in immunocompetent adults. The bacterium was identified by 16S rRNA gene sequencing. This is the third published report of the recovery of this organism from human clinical material and highlights the importance of the organism as a potential human patho...

journal_title：Journal of clinical microbiology

authors： Jiang W,Abrar S,Romagnoli M,Carroll KC

更新日期：2009-05-01 00:00:00

abstract：:A recent case report of a child infected with enterohemorrhagic Escherichia coli (EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211-213,...

journal_title：Journal of clinical microbiology

authors： Wieler LH,Busse B,Steinrück H,Beutin L,Weber A,Karch H,Baljer G

更新日期：2000-06-01 00:00:00

abstract：:Beagle dogs inoculated intravenously with 1.75 x 10(9) viable Encephalitozoon cuniculi spores at 12.5 months of age were monitored for 18 months to assess infection in the adult dog. Parameters monitored included packed cell volume, total and differential leukocyte counts, and humoral and cellular responses to infecti...

journal_title：Journal of clinical microbiology

authors： Szabo JR,Shadduck JA

更新日期：1988-03-01 00:00:00

abstract：:We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified wit...

journal_title：Journal of clinical microbiology

authors： Tiballi RN,He X,Zarins LT,Revankar SG,Kauffman CA

更新日期：1995-04-01 00:00:00

abstract：:We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes. ...

journal_title：Journal of clinical microbiology

authors： Ito K,Iida M,Yamazaki M,Moriya K,Moroishi S,Yatsuyanagi J,Kurazono T,Hiruta N,Ratchtrachenchai OA

更新日期：2007-03-01 00:00:00

abstract：:Chromosomal DNAs of selected Bacteroides organisms whose relatedness had been previously determined by "conventional" filter-annealing studies (J. L. Johnson, Int. J. Syst. Bacteriol. 28:245, 1978) were further analyzed by restriction endonuclease analysis coupled with the Southern hybridization procedure (E. M. South...

journal_title：Journal of clinical microbiology

authors： Bradbury WC,Murray RG,Mancini C,Morris VL

更新日期：1985-01-01 00:00:00

abstract：:Three new Salmonella serotypes belonging to Kauffmann's subgenus I (F. Kauffmann, The Bacteriology of Enterobacteriaceae, 1966) were identified. These serotypes were Salmonella brazos 6,14,18:a:e,n,z15, Salmonella midway 6,14,24:d:1,7, and Salmonella balboa 48a, 48b:z41:monophasic. ...

journal_title：Journal of clinical microbiology

authors： Sutch KE,Blackburn BO

更新日期：1980-06-01 00:00:00

abstract：:Reverse transcription-PCR (RT-PCR) is a sensitive method for detection of RNA virus nucleic acid sequences in clinical respiratory specimens. Previous studies have focused on RT-PCR for a single virus, but this approach is limited by the inability to establish a specific etiology when the RT-PCR result is negative and...

journal_title：Journal of clinical microbiology

authors： Gilbert LL,Dakhama A,Bone BM,Thomas EE,Hegele RG

更新日期：1996-01-01 00:00:00

abstract：:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

journal_title：Journal of clinical microbiology

authors： Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

更新日期：2014-12-01 00:00:00

abstract：:Among nine patients with bacteremia caused by Granulicatella or Gemella in a 6-year period (July 1995 to June 2001), three had bacteremia caused by erythromycin-resistant Granulicatella adiacens and one had bacteremia caused by erythromycin-resistant Gemella haemolysans. All four isolates possessed mef genes, whereas ...

journal_title：Journal of clinical microbiology

authors： Woo PC,To AP,Lau SK,Fung AM,Yuen KY

更新日期：2004-07-01 00:00:00

abstract：:We evaluated the abilities of three different avian influenza A viruses to attenuate the wild-type human influenza A/Korea/1/82 (H3N2) virus in squirrel monkeys, chimpanzees, and adult seronegative human volunteers. Two of these, avian influenza A/Mallard/NY/78 and A/Mallard/Alberta/76 viruses, appeared to be satisfac...

journal_title：Journal of clinical microbiology

authors： Snyder MH,Clements ML,Herrington D,London WT,Tierney EL,Murphy BR

更新日期：1986-09-01 00:00:00