Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia.

abstract：:We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin...

journal_title：Journal of clinical microbiology

authors： Murdoch DR,Mirrett S,Harrell LJ,Donabedian SM,Zervos MJ,Reller LB

更新日期：2003-06-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both...

journal_title：Journal of clinical microbiology

authors： Honda T,Sornchai C,Takeda Y,Miwatani T

更新日期：1982-10-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00

abstract：:The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. I...

journal_title：Journal of clinical microbiology

authors： Rudrik JT,Cavalieri SJ,Britt EM

更新日期：1985-03-01 00:00:00

abstract：:A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced by clinical isolates of enterotoxigenic E. coli (STa-ETEC) by a single monoclonal antibody-based inhibition GM1 enzyme-li...

journal_title：Journal of clinical microbiology

authors： Sanchez J,Holmgren J,Svennerholm AM

更新日期：1990-10-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:Of 532 strains of viridans streptococci isolated from clinical specimens, 517 were identified by using a scheme based on the work of Facklam (R. R. Facklam, J. Clin. Microbiol. 5:184-201, 1977). The strains were distributed among nine of the species described by Facklam and an additional group of physiologically homog...

journal_title：Journal of clinical microbiology

authors： Ruoff KL,Kunz LJ

更新日期：1982-05-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...

journal_title：Journal of clinical microbiology

authors： Haque NZ,Arshad S,Peyrani P,Ford KD,Perri MB,Jacobsen G,Reyes K,Scerpella EG,Ramirez JA,Zervos MJ

更新日期：2012-05-01 00:00:00

abstract：:Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for pri...

journal_title：Journal of clinical microbiology

authors： Johnson LD,Fuccillo DA,Stalder H,Oxman MA,Fraser CE,Madden DL

更新日期：1979-03-01 00:00:00

abstract：:Single colonies of beta-hemolytic streptococci could be grouped by antibody-coated latex bead agglutination or coagglutination with staphylococci coated with antibody after incubation of the colonies with mutanolysin. This simple and quick procedure provided an enzymatic means of enhancing the sensitivity of tests suc...

journal_title：Journal of clinical microbiology

authors： Calandra GB,Henson CL

更新日期：1982-01-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:Previous analysis of the microbiology of advanced caries by culture and real-time PCR emphasized the high incidence and abundance of gram-negative anaerobic species, particularly Prevotella-like bacteria. The diversity of Prevotella-like bacteria was further explored by analyzing pooled bacterial DNA from lesions of c...

journal_title：Journal of clinical microbiology

authors： Nadkarni MA,Caldon CE,Chhour KL,Fisher IP,Martin FE,Jacques NA,Hunter N

更新日期：2004-11-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00

abstract：:Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simula...

journal_title：Journal of clinical microbiology

authors： Poutrel B,Mendolia C,Sutra L,Fournier JM

更新日期：1990-02-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differenti...

journal_title：Journal of clinical microbiology

authors： Haas WH,Butler WR,Kirschner P,Plikaytis BB,Coyle MB,Amthor B,Steigerwalt AG,Brenner DJ,Salfinger M,Crawford JT,Böttger EC,Bremer HJ

更新日期：1997-12-01 00:00:00

abstract：:Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

journal_title：Journal of clinical microbiology

authors： Jabs DA,Forman M,Enger C,Jackson JB

更新日期：1999-05-01 00:00:00

abstract：:VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C(6)) with the IR(6) sequence was explored. Sensitivity was a...

journal_title：Journal of clinical microbiology

authors： Liang FT,Steere AC,Marques AR,Johnson BJ,Miller JN,Philipp MT

更新日期：1999-12-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00

abstract：:Of 382 strains tested, 261 strains of the species Bacteroides fragilis, B. thetaiotaomicron, B. ovatus, and B. distasonis produced phenylacetic acid; the remaining strains, belonging exclusively to the species B. vulgatus, failed to do so. This differentiation characteristic may be useful in routine clinical bacteriol...

journal_title：Journal of clinical microbiology

authors： van Assche PF

更新日期：1978-11-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:Respiratory tract infections are a leading cause of morbidity and mortality worldwide. Even with the advancement of diagnostic tools, the causative agent of 20 to 30% of upper respiratory tract infections go undiagnosed. Recently, a newly identified human respiratory virus, human metapneumovirus (hMPV), was discovered...

journal_title：Journal of clinical microbiology

authors： Regev L,Hindiyeh M,Shulman LM,Barak A,Levy V,Azar R,Shalev Y,Grossman Z,Mendelson E

更新日期：2006-04-01 00:00:00

abstract：:Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [C...

journal_title：Journal of clinical microbiology

authors： Lepine DG,Neumann PW,Frenette SL,O'Shaughnessy MV

更新日期：1990-06-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:A gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by Campylobacter spp. This technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chroma...

journal_title：Journal of clinical microbiology

authors： Bär W,Fricke G

更新日期：1987-09-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00