Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species.

abstract：:JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a central nervous system infection that mainly affects AIDS patients. The extensive application of highly active antiretroviral therapy (HAART) is leading to the appearance of "long-term" survival PML patients. A reliable...

journal_title：Journal of clinical microbiology

authors： García de Viedma D,Alonso R,Miralles P,Berenguer J,Rodriguez-Créixems M,Bouza E

更新日期：1999-03-01 00:00:00

abstract：:Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require redecontamination and reincubation before the appropriate tests can be performed for identification, significantly affecting the turnaround tim...

journal_title：Journal of clinical microbiology

authors： Zheng X,Pang M,Engler HD,Tanaka S,Reppun T

更新日期：2001-10-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for detection of immunoglobulin M (IgM) and IgG antibodies to Mycoplasma pneumoniae was developed. The EIA was evaluated on the basis of results in the M. pneumoniae complement fixation (MPCF) test and the cold agglutinin test. Serum samples from 430 patients with respiratory infections of ...

journal_title：Journal of clinical microbiology

authors： Uldum SA,Jensen JS,Søndergård-Andersen J,Lind K

更新日期：1992-05-01 00:00:00

abstract：:Because congenital toxoplasmosis is almost solely the result of maternal infection acquired during gestation, it is critical to determine whether infection during pregnancy has occurred. In the United States, definitive diagnosis of the acute infection and the time of its occurrence have been compromised by a lack of ...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Liesenfeld O,Kinney S,Press C,Remington JS

更新日期：2002-07-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Ovine progressive pneumonia virus (OPPV) is a lentivirus which causes a progressive disease in sheep. Immunodominant epitopes have been identified in the envelope gp40 glycoprotein. Synthetic peptides representing these regions are able to detect the presence of OPPV antibodies in 96% of infected sheep. ...

journal_title：Journal of clinical microbiology

authors： Kwang J,Torres JV

更新日期：1994-07-01 00:00:00

abstract：:Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, sub...

journal_title：Journal of clinical microbiology

authors： Manneck T,Braissant O,Haggenmüller Y,Keiser J

更新日期：2011-04-01 00:00:00

abstract：:Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

journal_title：Journal of clinical microbiology

authors： Escamilla J,Kilpatrick ME,Ugarte HF

更新日期：1986-01-01 00:00:00

abstract：:We compared the coreceptor tropism-predicting performance of a specific genotypic algorithm for HIV-1 subtype D and that of the geno2pheno algorithm with different cutoffs. The D-specific algorithm and geno2pheno with a false-positivity rate cutoff of 2.5% had the same concordance with the phenotypic determination. Th...

journal_title：Journal of clinical microbiology

authors： Dina J,Raymond S,Maillard A,Le Guillou-Guillemette H,Rodalec A,Beby-Defaux A,Giraudeau G,Vallet S,Mourez T,Payan C,Vabret A,Ruffault A,Ferre V,Izopet J,Plantier JC

更新日期：2013-09-01 00:00:00

abstract：:We enrolled 22 patients with gas-forming pyogenic liver abscess in a study to assess the mechanism of gas formation. Klebsiella pneumoniae was cultured from specimens from all patients. Gas and pus samples from abscesses revealed four major components: nitrogen, oxygen, carbon dioxide, and hydrogen; this implicates mi...

journal_title：Journal of clinical microbiology

authors： Lee HL,Lee HC,Guo HR,Ko WC,Chen KW

更新日期：2004-06-01 00:00:00

abstract：:Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulenc...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Kaster N,Kuskowski MA,Ling GV

更新日期：2003-01-01 00:00:00

abstract：:Fully standardized reproducible and sensitive quantification assays for cytomegalovirus (CMV) are needed to better define thresholds for antiviral therapy initiation and interruption. We evaluated the newly released Abbott RealTime CMV assay for CMV quantification in whole blood (WB) that includes automated extraction...

journal_title：Journal of clinical microbiology

authors： Schnepf N,Scieux C,Resche-Riggon M,Feghoul L,Xhaard A,Gallien S,Molina JM,Socié G,Viglietti D,Simon F,Mazeron MC,Legoff J

更新日期：2013-07-01 00:00:00

abstract：:The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >/=4 mg/liter to >/=0. 5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacilli...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Stoakes L,Massey V,Diagre D,Fitzgerald V,El Sayed S,Lannigan R

更新日期：2000-02-01 00:00:00

abstract：:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase,...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1983-10-01 00:00:00

abstract：:West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fev...

journal_title：Journal of clinical microbiology

authors： van Dam AP,van Gool T,Wetsteyn JC,Dankert J

更新日期：1999-06-01 00:00:00

abstract：:The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA). Consecutive urine specimens from 101 pregnant women and 287 nonpreg...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Jang D,Luinstra K,Faught M,Dalby D,Sellors J,Chernesky M

更新日期：1998-11-01 00:00:00

abstract：:Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results i...

journal_title：Journal of clinical microbiology

authors： Tsang RS,Sill ML,Advani A,Xing D,Newland P,Hallander H

更新日期：2005-05-01 00:00:00

abstract：:A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and si...

journal_title：Journal of clinical microbiology

authors： Zhou X,Kong F,Sorrell TC,Wang H,Duan Y,Chen SC

更新日期：2008-07-01 00:00:00

abstract：:Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

journal_title：Journal of clinical microbiology

authors： Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

更新日期：2000-07-01 00:00:00

abstract：:Subgroup J avian leukosis virus (ALV-J) is an avian retrovirus that causes severe economic losses in the poultry industry. The early identification and removal of virus-shedding birds are important to reduce the spread of congenital and contact infections. In this study, a TaqMan-based real-time PCR method for the rap...

journal_title：Journal of clinical microbiology

authors： Qin L,Gao Y,Ni W,Sun M,Wang Y,Yin C,Qi X,Gao H,Wang X

更新日期：2013-01-01 00:00:00

abstract：:Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

journal_title：Journal of clinical microbiology

authors： Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

更新日期：1996-01-01 00:00:00

abstract：:Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...

journal_title：Journal of clinical microbiology

authors： Kumar A,Shukla D,Kumar R,Idris MZ,Misra UK,Dhole TN

更新日期：2012-11-01 00:00:00

abstract：:One of the major unanswered questions regarding the presence of Listeria monocytogenes in foods is how many cells must be ingested in order to cause illness. To answer this question, studies were undertaken by using Macaca fascicularis (cynomolgus monkey) as an animal model. Healthy nonhuman primates were dosed with v...

journal_title：Journal of clinical microbiology

authors： Farber JM,Daley E,Coates F,Beausoleil N,Fournier J

更新日期：1991-11-01 00:00:00

abstract：:It was noted in our laboratory that certain strains of Haemophilus influenzae yielded zone sizes interpreted as resistant to the ampicillin (AMP) disk on chocolate-Mueller-Hinton agar (CMH) but showed no evidence of beta-lactamase (beta-Lac) activity. Although it is known that a second mechanism of AMP resistance exis...

journal_title：Journal of clinical microbiology

authors： Heelan JS,Chesney D,Guadagno G

更新日期：1992-07-01 00:00:00

abstract：:In a follow-up study, enterotoxigenic Escherichia coli (ETEC) infections in 145 children from two communities located in northeastern Argentina were monitored for 2 years. The occurrence of diarrhea was monitored by weekly household visits. Of 730 fecal specimens collected, 137 (19%) corresponded to diarrheal episodes...

journal_title：Journal of clinical microbiology

authors： Viboud GI,Jouve MJ,Binsztein N,Vergara M,Rivas M,Quiroga M,Svennerholm AM

更新日期：1999-09-01 00:00:00

abstract：:Alpha-toxin is a major Staphylococcus aureus virulence factor. This study evaluated potential relationships between in vitro alpha-toxin expression of S. aureus bloodstream isolates, anti-alpha-toxin antibody in serum of patients with S. aureus bacteremia (SAB), and clinical outcomes in 100 hemodialysis and 100 postsu...

journal_title：Journal of clinical microbiology

authors： Sharma-Kuinkel BK,Wu Y,Tabor DE,Mok H,Sellman BR,Jenkins A,Yu L,Jafri HS,Rude TH,Ruffin F,Schell WA,Park LP,Yan Q,Thaden JT,Messina JA,Fowler VG Jr,Esser MT

更新日期：2015-01-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction produ...

journal_title：Journal of clinical microbiology

authors： Lehmann PF,Lin D,Lasker BA

更新日期：1992-12-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...

journal_title：Journal of clinical microbiology

authors： Kawano J,Shimizu A,Saitoh Y,Yagi M,Saito T,Okamoto R

更新日期：1996-09-01 00:00:00

abstract：:Levels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and lim...

journal_title：Journal of clinical microbiology

authors： Sum SS,Wong DK,Yuen MF,Yuan HJ,Yu J,Lai CL,Ho D,Zhang L

更新日期：2004-08-01 00:00:00