Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide ligation assay on DNA chips.

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and ...

journal_title：Journal of clinical microbiology

authors： Himmelreich CA,Barenkamp SJ,Storch GA

更新日期：1985-02-01 00:00:00

abstract：:The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

journal_title：Journal of clinical microbiology

authors： Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

更新日期：1990-05-01 00:00:00

abstract：:During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Vibits H,Ursing J,Korner B

更新日期：1991-05-01 00:00:00

abstract：:A patient with a dual Salmonella infection is described. Salmonella group B was recovered from three blood culture sets but was not detected in seven stool cultures. Salmonella group C2 was isolated from three of seven stool cultures but was not recovered from blood cultures. Specific, non-cross-reactive antibodies to...

journal_title：Journal of clinical microbiology

authors： Papasian CJ,Bartholomew WR,Neter E,Amsterdam D

更新日期：1984-09-01 00:00:00

abstract：:The Binax legionella urinary antigen (LUA) enzyme immunoassay (Binax, Portland, Maine) was evaluated in 159 patients with suspected or proven legionellosis and 209 controls. A positive LUA test was found in 37% of patients with suspected legionellosis overall and in 83% of those with proven Legionella pneumophila sero...

journal_title：Journal of clinical microbiology

authors： Kazandjian D,Chiew R,Gilbert GL

更新日期：1997-04-01 00:00:00

abstract：:The performances of a commercially available qualitative plasma PCR assay (AMPLICOR CMV test; Roche Diagnostics) and the pp65 antigenemia assay (AG) were evaluated for the monitoring of cytomegalovirus (CMV) viremia in 43 allogeneic stem cell transplant recipients. In addition, the suitabilities of both assays for tri...

journal_title：Journal of clinical microbiology

authors： Solano C,Muñoz I,Gutiérrez A,Farga A,Prósper F,García-Conde J,Navarro D,Gimeno C

更新日期：2001-11-01 00:00:00

abstract：:The correlation between response to antiviral therapy and pretreatment viral load in patients with chronic hepatitis C has prompted the development of quantitative assays to measure viral load. The aim of our study was to assess the clinical relevance of the newly developed semiautomated PCR system COBAS HCV MONITOR v...

journal_title：Journal of clinical microbiology

authors： Martinot-Peignoux M,Le Breton V,Fritsch S,Le Guludec G,Labouret N,Keller F,Marcellin P

更新日期：2000-07-01 00:00:00

abstract：:By serological methods, all 83 known types of Streptococcus pneumoniae could be shown to possess C polysaccharide and F antigen. Cross-reactions due to these two antigens between pneumococci and a broad range of most other commonly encountered streptococci were examined. The presence of an antigen closely similar or i...

journal_title：Journal of clinical microbiology

authors： Sørensen UB,Henrichsen J

更新日期：1987-10-01 00:00:00

abstract：:We report the first documented case of endocarditis in a man infected with Bartonella alsatica, which causes bacteremia in healthy wild rabbits. B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negativ...

journal_title：Journal of clinical microbiology

authors： Raoult D,Roblot F,Rolain JM,Besnier JM,Loulergue J,Bastides F,Choutet P

更新日期：2006-01-01 00:00:00

abstract：:A new disposable plastic tube device and technique for ovum and parasite concentration, with upper and lower chambers separated by a fine screen, was compared with the standard gauze and open-funnel filtration technique as well as with the technique of direct smearing with an acid-fast stain. The new concentrator was ...

journal_title：Journal of clinical microbiology

authors： Zierdt WS

更新日期：1984-11-01 00:00:00

abstract：:The prevalence of coinfection of Campylobacter species in dogs was determined using four isolation methods. In 26% of the positive-testing stools, multiple Campylobacter species were identified. The use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection ...

journal_title：Journal of clinical microbiology

authors： Koene MG,Houwers DJ,Dijkstra JR,Duim B,Wagenaar JA

更新日期：2004-02-01 00:00:00

abstract：:Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide informa...

journal_title：Journal of clinical microbiology

authors： Sun Y,Kong F,Zhao Z,Gilbert GL

更新日期：2005-09-01 00:00:00

abstract：:On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from...

journal_title：Journal of clinical microbiology

authors： Schuetz AN,Hemarajata P,Mehta N,Campbell S,Mitchell S,Palavecino E,Butler-Wu S,Miller MB

更新日期：2020-12-17 00:00:00

abstract：:A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive b...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Venturoli S,Gallinella G,Gibellini D,Gentilomi G,La Placa M

更新日期：1990-11-01 00:00:00

abstract：:A commercial DNA probe kit designed to detect rRNA from legionellae was evaluated for its ability to correctly discriminate between legionellae and non-legionellae taken from culture plates. The probe kit, made by the Gen-Probe Corp. (San Diego, Calif.), was radiolabeled with 125I, and probe bacterial RNA hybridizatio...

journal_title：Journal of clinical microbiology

authors： Edelstein PH

更新日期：1986-03-01 00:00:00

abstract：:Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relativ...

journal_title：Journal of clinical microbiology

authors： Archambault D,East N,Perk K,Dahlberg JE

更新日期：1988-05-01 00:00:00

abstract：:Malassezia yeasts are associated with several dermatological disorders. The conventional identification of Malassezia species by phenotypic methods is complicated and time-consuming, and the results based on culture methods are difficult to interpret. A comparative molecular approach based on the use of three molecula...

journal_title：Journal of clinical microbiology

authors： Gupta AK,Boekhout T,Theelen B,Summerbell R,Batra R

更新日期：2004-09-01 00:00:00

abstract：:Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...

journal_title：Journal of clinical microbiology

authors： Geraats-Peters CW,Brouwers M,Schneeberger PM,van der Zanden AG,Bruisten SM,Weers-Pothoff G,Boel CH,van den Brule AJ,Harmsen HG,Hermans MH

更新日期：2005-11-01 00:00:00

abstract：:Many laboratory workers prefer the rhodamine-auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rho...

journal_title：Journal of clinical microbiology

authors： McCarter YS,Robinson A

更新日期：1994-10-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Kettlitz C,Werner G,Witte W

更新日期：2003-09-01 00:00:00

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. ...

journal_title：Journal of clinical microbiology

authors： Stone NR,Gorton RL,Barker K,Ramnarain P,Kibbler CC

更新日期：2013-04-01 00:00:00

abstract：:An organism resembling Corynebacterium striatum was isolated from the blood of a patient with acute aortic valvular insufficiency and no history of valvular heart disease. At autopsy, histopathologic examination of the aortic valve revealed pleomorphic gram-positive bacilli and destruction of valvular tissue. Our isol...

journal_title：Journal of clinical microbiology

authors： Markowitz SM,Coudron PE

更新日期：1990-01-01 00:00:00

abstract：:Fifteen monoclonal antibodies (MAbs) made to coxsackievirus B-3 were tested against a panel of enteroviruses by indirect immunofluorescence. The MAbs included seven which reacted with coxsackievirus B-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enterovi...

journal_title：Journal of clinical microbiology

authors： Yagi S,Schnurr D,Lin J

更新日期：1992-09-01 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus d...

journal_title：Journal of clinical microbiology

authors： Tremblay CL,Giguel F,Merrill DP,Wong JT,Rosenberg E,Kalams S,Walker BD,D'Aquila RT,Hirsch MS

更新日期：2000-11-01 00:00:00

abstract：:Over a decade ago the first cryptococcal lateral flow assay (LFA) became available. This affordable point-of-care test has undeniably led to tremendous improvements in early detection of cryptococcosis (1,2). However, from several studies it must be concluded that the reliability of recently introduced LFA's falls sho...

journal_title：Journal of clinical microbiology

authors： Shi D,Haas PJ,Boekhout T,Hahn RC,Hagen F

更新日期：2021-01-20 00:00:00