Carious dentine provides a habitat for a complex array of novel Prevotella-like bacteria.

abstract：:Blood culture contamination greatly affects clinical decisions. Hence, it is of interest to assess the influence of factors such as the volume of blood drawn and the site of blood draw on the rates of blood culture contamination. In a retrospective study, blood cultures from infants and children up to 18 years of age ...

journal_title：Journal of clinical microbiology

authors： Gonsalves WI,Cornish N,Moore M,Chen A,Varman M

更新日期：2009-11-01 00:00:00

abstract：:Candidate live-virus vaccines for respiratory syncytial virus are being developed and are beginning to be evaluated in clinical trials. To distinguish candidate vaccine strains from wild-type strains isolated during these trials, we developed PCR assays specific to two sets of candidate vaccine strains. The two sets w...

journal_title：Journal of clinical microbiology

authors： Zheng H,Peret TC,Randolph VB,Crowley JC,Anderson LJ

更新日期：1996-02-01 00:00:00

abstract：:Helicobacter pylori strains display remarkable genetic diversity, and the presence of strains bearing the toxigenic vacA s1 allele, a complete cag pathogenicity island (PAI), cagA alleles containing multiple EPIYA phosphorylation sites, and expressing the BabA adhesin correlates with development of gastroduodenal dise...

journal_title：Journal of clinical microbiology

authors： Talarico S,Gold BD,Fero J,Thompson DT,Guarner J,Czinn S,Salama NR

更新日期：2009-06-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:Among 234 isolates comprising 26 different Candida species colonizing the oropharynx of 181 (54.3% of 399 surveyed) HIV-infected outpatients, 27 (11.7%) were fluconazole resistant. Antibacterial treatment was associated with increased rates of yeast colonization, while antiretroviral therapy and pneumococcal vaccinati...

journal_title：Journal of clinical microbiology

authors： Yang YL,Hung CC,Wang AH,Tseng FC,Leaw SN,Tseng YT,Su CL,Chen HT,Lauderdale TL,Lo HJ

更新日期：2010-07-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00

abstract：:psaA is the gene encoding pneumococcal surface adhesin A (PsaA), a 37-kDa protein expressed on the surface of Streptococcus pneumoniae. PCR primers for psaA have been shown to amplify the target DNA sequence in all 90 serotypes of S. pneumoniae and in none of 67 heterologous pathogens and colonizing bacteria of the up...

journal_title：Journal of clinical microbiology

authors： Scott JA,Marston EL,Hall AJ,Marsh K

更新日期：2003-06-01 00:00:00

abstract：:Clinically important species of Candida and related organisms were compared for extent of nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA (rDNA) gene. This rDNA region is sufficiently variable to allow reliable separation of all known clinically significant yeast species. Of the 204 descri...

journal_title：Journal of clinical microbiology

authors： Kurtzman CP,Robnett CJ

更新日期：1997-05-01 00:00:00

abstract：:Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and ...

journal_title：Journal of clinical microbiology

authors： Holliday MG,Ford M,Perry JD,Gould FK

更新日期：1999-04-01 00:00:00

abstract：:A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...

journal_title：Journal of clinical microbiology

authors： Dhar AK,Roux MM,Klimpel KR

更新日期：2001-08-01 00:00:00

abstract：:Whether targeted or universal decolonization strategies for the control of methicillin-resistant Staphylococcus aureus (MRSA) select for resistance to decolonizing agents is unresolved. The REDUCE-MRSA trial (ClinicalTrials registration no. NCT00980980) provided an opportunity to investigate this question. REDUCE-MRSA...

journal_title：Journal of clinical microbiology

authors： Hayden MK,Lolans K,Haffenreffer K,Avery TR,Kleinman K,Li H,Kaganov RE,Lankiewicz J,Moody J,Septimus E,Weinstein RA,Hickok J,Jernigan J,Perlin JB,Platt R,Huang SS

更新日期：2016-11-01 00:00:00

abstract：:We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

journal_title：Journal of clinical microbiology

authors： Cheah FC,Anderson TP,Darlow BA,Murdoch DR

更新日期：2005-01-01 00:00:00

abstract：:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human corona...

journal_title：Journal of clinical microbiology

authors： Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

更新日期：2015-08-01 00:00:00

abstract：:Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Cl...

journal_title：Journal of clinical microbiology

authors： Baldellon C,Mégraud F

更新日期：1985-03-01 00:00:00

abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

journal_title：Journal of clinical microbiology

authors： Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

更新日期：2012-12-01 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:Discovery of bats with severe acute respiratory syndrome (SARS)-related coronaviruses (CoVs) raised the specter of potential future outbreaks of zoonotic SARS-CoV-like disease in humans, which largely went unheeded. Nevertheless, the novel SARS-CoV-2 of bat ancestral origin emerged to infect humans in Wuhan, China, in...

journal_title：Journal of clinical microbiology

authors： Saif LJ,Jung K

更新日期：2020-07-23 00:00:00

abstract：:Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...

journal_title：Journal of clinical microbiology

authors： Welch DF,Hensel DM,Pickett DA,San Joaquin VH,Robinson A,Slater LN

更新日期：1993-09-01 00:00:00

abstract：:The nasopharyngeal Haemophilus influenzae flora of healthy children in a day care center was analyzed by repeated sampling during 4 winter months. The average carrier rate was 39%, but 74% of the children became colonized at some time during the study. The H. influenzae isolates were identified by capsular type, bioty...

journal_title：Journal of clinical microbiology

authors： Trottier S,Stenberg K,Svanborg-Edén C

更新日期：1989-10-01 00:00:00

abstract：:The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle ...

journal_title：Journal of clinical microbiology

authors： Wellenberg GJ,Verstraten ER,Mars MH,Van Oirschot JT

更新日期：1998-02-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:The prevalence of tick-borne infections has been steadily increasing in both number and geographic distribution in the United States and abroad. This increase, in conjunction with the continued recognition of novel pathogens transmitted by ticks, has made accurate diagnosis of these infections challenging. Mainstay se...

journal_title：Journal of clinical microbiology

authors： Buchan BW,Jobe DA,Mashock M,Gerstbrein D,Faron ML,Ledeboer NA,Callister SM

更新日期：2019-10-23 00:00:00

abstract：:Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the...

journal_title：Journal of clinical microbiology

authors： Kozyreva VK,Truong CL,Greninger AL,Crandall J,Mukhopadhyay R,Chaturvedi V

更新日期：2017-08-01 00:00:00

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect Legionella species in bronchoalveolar fluid specimens. All Legionella strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Reinthaler FF,Pschaid A,Pierer K,Kleinhappl B,Eber E,Marth E

更新日期：1993-12-01 00:00:00

abstract：:Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

journal_title：Journal of clinical microbiology

authors： Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

更新日期：1994-05-01 00:00:00

abstract：:A LightCycler-based PCR protocol was developed which targets the ospA gene for the identification and quantification of the different Borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (HybProbe) and an internally labeled primer. The detection limit of th...

journal_title：Journal of clinical microbiology

authors： Rauter C,Oehme R,Diterich I,Engele M,Hartung T

更新日期：2002-01-01 00:00:00

abstract：:West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fev...

journal_title：Journal of clinical microbiology

authors： van Dam AP,van Gool T,Wetsteyn JC,Dankert J

更新日期：1999-06-01 00:00:00

abstract：:Between 1999 and 2006, 15 cats were diagnosed with disease attributable to a novel mycobacterial species. The infections consisted of granulomatous lesions in the skin, subcutis, and ocular or periocular tissues with an indolent but progressive clinical course. Lesions typically were found in facial regions or on the ...

journal_title：Journal of clinical microbiology

authors： Fyfe JA,McCowan C,O'Brien CR,Globan M,Birch C,Revill P,Barrs VR,Wayne J,Hughes MS,Holloway S,Malik R

更新日期：2008-02-01 00:00:00

abstract：:The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

journal_title：Journal of clinical microbiology

authors： Woods GL,LaTemple D,Cruz C

更新日期：1994-04-01 00:00:00