Abstract:
:West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In vitro experiments showed that QBC analysis may be as much as 100-fold more sensitive than thick smear. Spirochetes were also cultured from blood samples from both patients in modified Kelly's medium and were identified as B. crocidurae by partial sequencing of the PCR-amplified rrs gene.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
van Dam AP,van Gool T,Wetsteyn JC,Dankert Jdoi
10.1128/JCM.37.6.2027-2030.1999subject
Has Abstractpub_date
1999-06-01 00:00:00pages
2027-30issue
6eissn
0095-1137issn
1098-660Xjournal_volume
37pub_type
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