Typing of human Mycobacterium avium isolates in Italy by IS1245-based restriction fragment length polymorphism analysis.

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that d...

journal_title：Journal of clinical microbiology

authors： Pisanic N,Randad PR,Kruczynski K,Manabe YC,Thomas DL,Pekosz A,Klein SL,Betenbaugh MJ,Clarke WA,Laeyendecker O,Caturegli PP,Larman HB,Detrick B,Fairley JK,Sherman AC,Rouphael N,Edupuganti S,Granger DA,Granger SW,Coll

更新日期：2020-12-17 00:00:00

abstract：:A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Dodge DE,Pierer K,Young KK,Liao Y,Santner BI,Eber E,Roeger MG,Stuenzner D,Sixl-Voigt B,Marth E

更新日期：1997-06-01 00:00:00

abstract：:We evaluated a commercially available second-generation anti-H. pylori immunoglobulin G enzyme immunoassay (EIA) (Cobas Core Anti-Helicobacter pylori EIA; Roche S. A., Basel, Switzerland) for serodiagnosis of H. pylori infection. The results of the assay were assessed in relation to the results of bacterial culture, u...

journal_title：Journal of clinical microbiology

authors： Goossens H,Glupczynski Y,Burette A,Van den Borre C,Butzler JP

更新日期：1992-01-01 00:00:00

abstract：:Adhesin-encoding operons (pap, sfa/foc, and afa) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. A quick and sensitive assay to identify these operons was developed by using the polymerase chain reaction (PCR). Three pairs of 25-mer primers were defined from the se...

journal_title：Journal of clinical microbiology

authors： Le Bouguenec C,Archambaud M,Labigne A

更新日期：1992-05-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

journal_title：Journal of clinical microbiology

authors： Goldwater PN

更新日期：1995-06-01 00:00:00

abstract：:We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity ...

journal_title：Journal of clinical microbiology

authors： Fiscus SA,Brambilla D,Grosso L,Schock J,Cronin M

更新日期：1998-01-01 00:00:00

abstract：:Monitoring antiretroviral therapy requires that human immunodeficiency virus type 1 (HIV-1) viremia assays are applicable to all distinct variants. This study evaluates the performance of three commercial viral load assays-Versant HIV-1 RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2-...

journal_title：Journal of clinical microbiology

authors： Holguín A,López M,Molinero M,Soriano V

更新日期：2008-09-01 00:00:00

abstract：:A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to...

journal_title：Journal of clinical microbiology

authors： Delisle GJ,Ley A

更新日期：1989-04-01 00:00:00

abstract：:We evaluated the performance and the cost of toxigenic culture using a commercial chromogenic medium (CDIF) for 538 stool specimens. Compared with real-time PCR, this method was found to detect an additional 9% of positive specimens and result in 61% reduction in material costs, with a trade-off increase in turnaround...

journal_title：Journal of clinical microbiology

authors： Luk S,To WK,Ng TK,Hui WT,Lee WK,Lau F,Ching AM

更新日期：2014-02-01 00:00:00

abstract：:A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobul...

journal_title：Journal of clinical microbiology

authors： Saijo M,Niikura M,Morikawa S,Kurane I

更新日期：2001-02-01 00:00:00

abstract：:We implemented hospital information system (HIS) alerts to deter unnecessary test orders for ovum and parasite (O&P) exams and Clostridium difficile PCR. The HIS alerts decreased noncompliant O&P orders (orders after >72 h of hospitalization) from 49.8% to 30.9%, an overall decrease of 19%, and reduced noncompliant C....

journal_title：Journal of clinical microbiology

authors： Otto CC,Shuptar SL,Milord P,Essick CJ,Nevrekar R,Granovsky SL,Seo SK,Babady NE,Martin SC,Tang YW,Pessin MS

更新日期：2015-08-01 00:00:00

abstract：:Meningococcal disease caused by N. meningitidis serogroup B (MenB) has been endemic in Brazil since 1997. In this study, we determined the prevalence of serosubtypes of MenB isolated in 10 Brazilian states and the Federal District during 1997 and 1998 and investigated the extent of PorA VR sequence variation among the...

journal_title：Journal of clinical microbiology

authors： Sacchi CT,Lemos AP,Popovic T,De Morais JC,Whitney AM,Melles CE,Brondi LM,Monteiro LM,Paiva MV,Solari CA,Mayer LW

更新日期：2001-08-01 00:00:00

abstract：:An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in s...

journal_title：Journal of clinical microbiology

authors： Roosendaal R,Vos JH,Roumen T,van Vugt R,Cattoli G,Bart A,Klaasen HL,Kuipers EJ,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2000-07-01 00:00:00

abstract：:The diagnosis of syphilis is challenging and often relies on serologic tests to detect treponemal or nontreponemal antibodies. Recently, the Centers for Disease Control and Prevention and the Association of Public Health Laboratories proposed an update to the syphilis serology testing algorithm, in which serum samples...

journal_title：Journal of clinical microbiology

authors： Binnicker MJ,Jespersen DJ,Rollins LO

更新日期：2011-04-01 00:00:00

abstract：:Whole-cell sonicates of Treponema pallidum, Nichols strain, were evaluated in an enzyme-linked immunosorbent assay (Elisa) for syphilis, and results were read in a Dynatek Microelisa Reader. The antigen was evaluated with sera from patients with syphilis, persons presumed normal, and biological false-positives. Two hu...

journal_title：Journal of clinical microbiology

authors： Pope V,Hunter EF,Feeley JC

更新日期：1982-04-01 00:00:00

abstract：:We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...

journal_title：Journal of clinical microbiology

authors： Rabella N,Drew WL

更新日期：1990-04-01 00:00:00

abstract：:A total of 101 isolates of penicillinase-producing and non-penicillinase-producing Neisseria gonorrhoeae with known nutritional requirements, plasmid content, and serovars, were examined for lectin agglutination patterns. These isolates were from outbreaks in Georgia, California, Hawaii, and Pennsylvania. Cell suspens...

journal_title：Journal of clinical microbiology

authors： Schalla WO,Whittington WL,Rice RJ,Larsen SA

更新日期：1985-09-01 00:00:00

abstract：:Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Le TA,Fabre L,Roumagnac P,Grimont PA,Scavizzi MR,Weill FX

更新日期：2007-11-01 00:00:00

abstract：:Monoclonal antibodies were generated from mice immunized with scolex protein antigen of Cysticercus cellulosae. Three monoclonal antibodies specific for cysticercal antigens, which did not show any cross-reactivity with Taenia solium or Taenia saginata antigens, were selected. Each monoclonal antibody coupled to Sepha...

journal_title：Journal of clinical microbiology

authors： Nascimento E,Tavares CA,Lopes JD

更新日期：1987-07-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:Antiretroviral therapy (ART) during acute HIV infection (AHI) interrupts viral dynamics and may delay the emergence of serological markers targeted by current HIV screening and confirmatory assays, thus creating challenges for correctly classifying HIV infection status. The performance of three HIV antigen/antibody co...

journal_title：Journal of clinical microbiology

authors： Manak MM,Jagodzinski LL,Shutt A,Malia JA,Leos M,Ouellette J,Akapirat S,Colby DL,Phanuphak N,Eller LA,Robb ML,de Souza M,Ananworanich J,Peel SA,RV254\/SEARCH010 and the RV217 Study Teams.

更新日期：2019-09-24 00:00:00

abstract：:Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six ot...

journal_title：Journal of clinical microbiology

authors： Miyakawa Y,Kagaya K,Fukazawa Y,Soe G

更新日期：1986-05-01 00:00:00

abstract：:Pseudomonas gladioli was isolated from 11 patients with cystic fibrosis. It resembled Pseudomonas cepacia on the selective and differential medium OFPBL, producing yellow colonies after 48 to 72 h of incubation. Isolates were characterized biochemically, by DNA hybridization, and by cellular fatty acid analysis. A rev...

journal_title：Journal of clinical microbiology

authors： Christenson JC,Welch DF,Mukwaya G,Muszynski MJ,Weaver RE,Brenner DJ

更新日期：1989-02-01 00:00:00

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

更新日期：2005-05-01 00:00:00

abstract：:Few studies have assessed the time to blood culture positivity as a predictor of clinical outcome in bloodstream infections (BSIs). The purpose of this study was to evaluate the time to positivity (TTP) of blood cultures in patients with Staphylococcus aureus BSIs and to assess its impact on clinical outcome. We perfo...

journal_title：Journal of clinical microbiology

authors： Marra AR,Edmond MB,Forbes BA,Wenzel RP,Bearman GM

更新日期：2006-04-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00