Strain-specific reverse transcriptase PCR assay: means to distinguish candidate vaccine from wild-type strains of respiratory syncytial virus.

abstract：:The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifyin...

journal_title：Journal of clinical microbiology

authors： Arribas JR,Clifford DB,Fichtenbaum CJ,Roberts RL,Powderly WG,Storch GA

更新日期：1995-06-01 00:00:00

abstract：:The description and evaluation of a PCR-based assay for the detection and species identification of the eight known human herpesviruses are presented. Two primer pairs targeting well-conserved regions of the genome allowed the amplification of the DNAs of all known human herpesviruses at a high level of sensitivity (1...

journal_title：Journal of clinical microbiology

authors： Johnson G,Nelson S,Petric M,Tellier R

更新日期：2000-09-01 00:00:00

abstract：:During a previous survey, five extended-spectrum beta-lactamase (ESBL)-producing enterobacteria (ESBLE) (two Enterobacter aerogenes isolates expressing TEM-24 b, two Escherichia coli isolates expressing TEM-21 or TEM-24 b, and one Klebsiella pneumoniae isolate expressing SHV-4/TEM-15) responsible for urinary tract inf...

journal_title：Journal of clinical microbiology

authors： Arpin C,Dubois V,Maugein J,Jullin J,Dutilh B,Brochet JP,Larribet G,Fischer I,Quentin C

更新日期：2005-10-01 00:00:00

abstract：:Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used f...

journal_title：Journal of clinical microbiology

authors： de Oña M,Melón S,de la Iglesia P,Hidalgo F,Verdugo AF

更新日期：1995-07-01 00:00:00

abstract：:A case of septicemia caused by Pseudomonas stutzeri belonging to the unusual biotype Vb-3 in a patient with multiple myeloma is described. The origin of the septicemia was attributed to a community-acquired pneumonia. The bacteriology and pathogenicity of P. stutzeri are reviewed. ...

journal_title：Journal of clinical microbiology

authors： Potvliege C,Jonckheer J,Lenclud C,Hansen W

更新日期：1987-02-01 00:00:00

abstract：:PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 week...

journal_title：Journal of clinical microbiology

authors： Wang SX,Sng LH,Leong HN,Tan BH

更新日期：2004-07-01 00:00:00

abstract：:By the use of enzyme immunoassay and RNA electrophoresis, rotavirus serotyping was performed with stool specimens from 605 children. Serotypes 1, 2, 3, and 4 accounted for 63, 9, 9, and 19% of the cases, respectively, with considerable yearly variation. An average of three serotypes cocirculated each year. ...

journal_title：Journal of clinical microbiology

authors： Begue RE,Dennehy PH,Huang J,Martin P

更新日期：1992-06-01 00:00:00

abstract：:Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

journal_title：Journal of clinical microbiology

authors： Beckwith DG,Jahre JA

更新日期：1980-10-01 00:00:00

abstract：:Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis. A total of three patients of Plasmodium ovale infection were detected, but PCR-amplified DNA of the P. ovale isolate...

journal_title：Journal of clinical microbiology

authors： Kawamoto F,Miyake H,Kaneko O,Kimura M,Nguyen TD,Nguyen TD,Liu Q,Zhou M,Le DD,Kawai S,Isomura S,Wataya Y

更新日期：1996-09-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00

abstract：:Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...

journal_title：Journal of clinical microbiology

authors： Mathai E,John TJ,Rani M,Mathai D,Chacko N,Nath V,Cherian AM

更新日期：1995-07-01 00:00:00

abstract：:Antibiotic-associated hemorrhagic colitis (AAHC) is associated with Klebsiella oxytoca. This study analyzed whether cytotoxic properties are linked to specific subtypes of K. oxytoca. Klebsiella isolates from stools of AAHC patients, healthy carriers, and diarrhea patients as well as from infections of other organs we...

journal_title：Journal of clinical microbiology

authors： Joainig MM,Gorkiewicz G,Leitner E,Weberhofer P,Zollner-Schwetz I,Lippe I,Feierl G,Krause R,Hinterleitner T,Zechner EL,Högenauer C

更新日期：2010-03-01 00:00:00

abstract：:In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity a...

journal_title：Journal of clinical microbiology

authors： Inouye S,Hasegawa A,Matsuno S,Katow S

更新日期：1984-09-01 00:00:00

abstract：:The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

journal_title：Journal of clinical microbiology

authors： Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

更新日期：2011-11-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay was developed for its potential utility in the detection of antigen in the cerebrospinal fluid of patients with tuberculous meningitis. Cerebrospinal fluids examined included those from untreated (group Ia) and treated (group Ib) Mycobacterium tuberculosis meningitis, nonse...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Mazarelo TB,Chaparas SD

更新日期：1986-05-01 00:00:00

abstract：:Eighty-seven strains of Pseudomonas aeruginosa were typed by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments. Stains were clustered on the basis of interpretative criteria as presented previously for the PFGE analysis. Clusters of strains were al...

journal_title：Journal of clinical microbiology

authors： Renders N,Römling U,Verbrugh H,van Belkum A

更新日期：1996-12-01 00:00:00

abstract：:The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal puls...

journal_title：Journal of clinical microbiology

authors： Morrison D,Woodford N,Barrett SP,Sisson P,Cookson BD

更新日期：1999-04-01 00:00:00

abstract：:The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-...

journal_title：Journal of clinical microbiology

authors： Willinger B,Obradovic A,Selitsch B,Beck-Mannagetta J,Buzina W,Braun H,Apfalter P,Hirschl AM,Makristathis A,Rotter M

更新日期：2003-02-01 00:00:00

abstract：:The conventional phage typing scheme proposed by S. Basu and S. Mukerjee (Experientia 24:299-300, 1968) has been used routinely for identification of the strains at the Vibrio Phage Reference Laboratory since 1968. However, because of limitations of this scheme, a new phage typing scheme using five newly isolated phag...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay DJ,Sarkar BL,Ansari MQ,Chakrabarti BK,Roy MK,Ghosh AN,Pal SC

更新日期：1993-06-01 00:00:00

abstract：:Sera from patients with allergic bronchopulmonary aspergillosis (ABPA) or aspergilloma and normal sera were analyzed for specific antibodies by Western (immuno-) blotting with Aspergillus fumigatus antigens transferred electrophoretically onto polyvinylidene difluoride membranes. Western blot analysis demonstrated con...

journal_title：Journal of clinical microbiology

authors： Kurup VP,Greenberger PA,Fink JN

更新日期：1989-06-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:Viable Angiostrongylus cantonensis was recovered from the cerebrospinal fluid of a 17-month-old boy with eosinophilic meningitis. Neurological findings were minimal, and the child had an uneventful recovery. ...

journal_title：Journal of clinical microbiology

authors： Kuberski T,Bart RD,Briley JM,Rosen L

更新日期：1979-05-01 00:00:00

abstract：:Acinetobacter soli is a new bacterial species described from forest soil. Five cases of bloodstream infection caused by A. soli clonal isolates are reported here for the first time. The patients were neonates admitted to an intensive care unit. This is a new neonatal pathogen with the potential to cause outbreaks. ...

journal_title：Journal of clinical microbiology

authors： Pellegrino FL,Vieira VV,Baio PV,dos Santos RM,dos Santos AL,Santos NG,Meohas MM,Santos RT,de Souza TC,da Silva Dias RC,Santoro-Lopes G,Riley LW,Moreira BM

更新日期：2011-06-01 00:00:00

abstract：:A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients wi...

journal_title：Journal of clinical microbiology

authors： Lee JC,Liu MJ,Parsonnet J,Arbeit RD

更新日期：1990-12-01 00:00:00

abstract：:Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respect...

journal_title：Journal of clinical microbiology

authors： Sawada T,Rimler RB,Rhoades KR

更新日期：1982-05-01 00:00:00

abstract：:We describe a central venous catheter-related (Port-A-Cath; Smiths Industries Medical Systems [SIMS] Deltec, Inc., St. Paul, Minn.) infection caused by Rhodotorula glutinis in a 51-year-old man with nasopharyngeal carcinoma. He was treated with fluconazole for 8 weeks and had the catheter removed. Two isolates of R. g...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Ho SW,Luh KT

更新日期：2003-02-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:We present a case of fever, brain abscesses, and Gemella morbillorum bacteremia after anti-tumor necrosis factor alpha (TNF-α) therapy in a 21-year-old acne inversa patient currently taking long-term dapsone. To the best of our knowledge, this is the first report describing such a case. During antimicrobial therapy, t...

journal_title：Journal of clinical microbiology

authors： Vossen MG,Gattringer KB,Khalifeh N,Koreny M,Spertini V,Mallouhi A,Willeit M,Volc-Platzer B,Asboth F,Graninger W,Thalhammer F,Lagler H

更新日期：2012-03-01 00:00:00

abstract：:Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...

journal_title：Journal of clinical microbiology

authors： Mitchell C,Jennings C,Brambilla D,Aldrovandi G,Amedee AM,Beck I,Bremer JW,Coombs R,Decker D,Fiscus S,Fitzgibbon J,Luzuriaga K,Moye J,Palumbo P,Reichelderfer P,Somasundaran M,Stevens W,Frenkel L,Dried Blood Spot Workin

更新日期：2008-09-01 00:00:00