Diminished human immunodeficiency virus type 1 DNA yield from dried blood spots after storage in a humid incubator at 37 degrees C compared to -20 degrees C.

abstract：:A protein antigen with an apparent molecular weight (Mr) of 31,000 was isolated from 0.2 M glycine hydrochloride (pH 2.2) extracts of a typical human fecal isolate, Campylobacter jejuni VC74. The protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving w...

journal_title：Journal of clinical microbiology

authors： Dubreuil JD,Kostrzynska M,Logan SM,Harris LA,Austin JW,Trust TJ

更新日期：1990-06-01 00:00:00

abstract：:We report the first documented case of canine strangles due to infection with Streptococcus equi in a dog with enlarged lymph nodes. Genetic typing, via sequencing of 12 housekeeping genes and the SeM gene, demonstrated the isolate to be a member of a common equine strain type circulating in the United Kingdom. ...

journal_title：Journal of clinical microbiology

authors： Ladlow J,Scase T,Waller A

更新日期：2006-07-01 00:00:00

abstract：:Using data from an ongoing Swedish intervention project, the observed durations of nasopharyngeal carriage of penicillin-nonsusceptible Streptococcus pneumoniae (PNSP) (MIC of penicillin G of >or=0.5 microg/ml) stratified by both pneumococcal serogroup and age of the carrier were compared. The means and 95% confidence...

journal_title：Journal of clinical microbiology

authors： Högberg L,Geli P,Ringberg H,Melander E,Lipsitch M,Ekdahl K

更新日期：2007-03-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

journal_title：Journal of clinical microbiology

authors： Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

更新日期：1996-06-01 00:00:00

abstract：:A panel (ENVA-1) of well-defined blinded samples containing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase was analyzed by automated DNA sequencing in 23 laboratories worldwide. Drug resistance mutations at codons 41, 215, and 184 were present in the panel samples at different r...

journal_title：Journal of clinical microbiology

authors： Schuurman R,Demeter L,Reichelderfer P,Tijnagel J,de Groot T,Boucher C

更新日期：1999-07-01 00:00:00

abstract：:A monoclonal antibody (MAb) immunoglobulin G2a (2125) was produced against a 60-kDa Legionella heat shock protein (HSP), recognizing a unique epitope common to all species of the genus Legionella. The antibody reacted in the immunoblot with 59 Legionella species and serogroups that were tested and showed no cross-reac...

journal_title：Journal of clinical microbiology

authors： Steinmetz I,Rheinheimer C,Hübner I,Bitter-Suermann D

更新日期：1991-02-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were ...

journal_title：Journal of clinical microbiology

authors： van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim B

更新日期：2014-12-01 00:00:00

abstract：:The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

journal_title：Journal of clinical microbiology

authors： Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

更新日期：2018-11-27 00:00:00

abstract：:Due to the inadequate automation in the amplification and sequencing procedures, the use of 16S rRNA gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. In this study, using conventional full-sequence 16...

journal_title：Journal of clinical microbiology

authors： Woo PC,Ng KH,Lau SK,Yip KT,Fung AM,Leung KW,Tam DM,Que TL,Yuen KY

更新日期：2003-05-01 00:00:00

abstract：:Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and ...

journal_title：Journal of clinical microbiology

authors： Holliday MG,Ford M,Perry JD,Gould FK

更新日期：1999-04-01 00:00:00

abstract：:Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

journal_title：Journal of clinical microbiology

authors： Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

更新日期：2005-08-01 00:00:00

abstract：:During pregnancy, Plasmodium falciparum infection of the placenta frequently occurs in the absence of parasites in peripheral blood. We investigated the abilities of the OptiMAL rapid immunochromatographic strip test for P. falciparum lactate dehydrogenase and species-specific PCR performed on peripheral blood to dete...

journal_title：Journal of clinical microbiology

authors： Mankhambo L,Kanjala M,Rudman S,Lema VM,Rogerson SJ

更新日期：2002-01-01 00:00:00

abstract：:The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...

journal_title：Journal of clinical microbiology

authors： Piccolomini R,Di Bonaventura G,Neri M,Di Girolamo A,Catamo G,Pizzigallo E

更新日期：1999-01-01 00:00:00

abstract：:A laboratory-based surveillance network of 11 clinical virological laboratories for influenza viruses was established in Taiwan under the coordination of the Center for Disease Control and Prevention (CDC), Taiwan. From October 2000 to March 2004, 3,244 influenza viruses were isolated, including 1,969 influenza A and ...

journal_title：Journal of clinical microbiology

authors： Shih SR,Chen GW,Yang CC,Yang WZ,Liu DP,Lin JH,Chiu SC,Chen HY,Tsao KC,Huang CG,Huang YL,Mok CK,Chen CJ,Lin TY,Wang JR,Kao CL,Lin KH,Chen LK,Eng HL,Liu YC,Chen PY,Lin JS,Wang JH,Lin CW,Chan YJ,Lu JJ,Hsi

更新日期：2005-04-01 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Vibits H,Ursing J,Korner B

更新日期：1991-05-01 00:00:00

abstract：:The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC...

journal_title：Journal of clinical microbiology

authors： Beld M,Minnaar R,Weel J,Sol C,Damen M,van der Avoort H,Wertheim-van Dillen P,van Breda A,Boom R

更新日期：2004-07-01 00:00:00

abstract：:Rapid detection of drug-resistant bacteria in clinical samples plays an instrumental role in patients' infection management and in implementing effective infection control policies. In the study described in this report, we validated a multiplex TaqMan real-time quantitative PCR (qPCR) assay for the detection of bla(K...

journal_title：Journal of clinical microbiology

authors： Hindiyeh M,Smollan G,Grossman Z,Ram D,Robinov J,Belausov N,Ben-David D,Tal I,Davidson Y,Shamiss A,Mendelson E,Keller N

更新日期：2011-07-01 00:00:00

abstract：:In this study, we have developed a reverse transcription (RT)-nested polymerase chain reaction (n-PCR) for the detection of mumps virus RNA in cerebrospinal fluid (CSF) from patients with neurological infections. A specific 112-bp fragment was amplified by this method with primers from the nucleoprotein of the mumps v...

journal_title：Journal of clinical microbiology

authors： Poggio GP,Rodriguez C,Cisterna D,Freire MC,Cello J

更新日期：2000-01-01 00:00:00

abstract：:The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Bernard CN

更新日期：2000-09-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relativ...

journal_title：Journal of clinical microbiology

authors： Archambault D,East N,Perk K,Dahlberg JE

更新日期：1988-05-01 00:00:00

abstract：:This study demonstrates the dynamics in the epidemiology of hepatitis C virus subtypes. Subtypes 3a and 4a have become increasingly prevalent in patients where an infection within recent years can be assumed. Evidence is presented that the subtypes observed among younger patients can spread rapidly and lead to signifi...

journal_title：Journal of clinical microbiology

authors： Schröter M,Zöllner B,Schäfer P,Reimer A,Müller M,Laufs R,Feucht HH

更新日期：2002-05-01 00:00:00

abstract：:Multi-virulence-locus sequence typing (MVLST) was used to analyze isolates from two major listeriosis outbreaks in the United States in 1998 and 2002 that were due to consumption of contaminated hot dogs and turkey deli meat, respectively. MVLST demonstrated high epidemiological relevance and indicated that the two ou...

journal_title：Journal of clinical microbiology

authors： Chen Y,Zhang W,Knabel SJ

更新日期：2005-10-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00

abstract：:Ehrlichia risticii causes an acute infectious disease in horses called Potomac horse fever. To investigate the biological diversity of E. risticii organisms, nine E. risticii isolates derived from the peripheral blood monocytes of clinically sick horses in Ohio and Kentucky during the summers of 1991 and 1993 were com...

journal_title：Journal of clinical microbiology

authors： Chaichanasiriwithaya W,Rikihisa Y,Yamamoto S,Reed S,Crawford TB,Perryman LE,Palmer GH

更新日期：1994-12-01 00:00:00

abstract：:Disk diffusion tests, inoculated directly from positive blood cultures, were evaluated for accuracy of reading zone diameters after 4- and 6-h and overnight incubation. In comparisons with results from standard disk diffusion tests, the 4-h results were in agreement for 83% of tests with gram-positive organisms and 64...

journal_title：Journal of clinical microbiology

authors： Coyle MB,McGonagle LA,Plorde JJ,Clausen CR,Schoenknecht FD

更新日期：1984-09-01 00:00:00