Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay.

abstract：:Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE tria...

journal_title：Journal of clinical microbiology

authors： Ssengooba W,Iragena JD,Nakiyingi L,Mujumbi S,Wobudeya E,Mboizi R,Boulware D,Meya DB,Choo L,Crook AM,Lebeau K,Joloba M,Demers AM,Cresswell FV,Gibb DM

更新日期：2020-08-24 00:00:00

abstract：:Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...

journal_title：Journal of clinical microbiology

authors： Nakata S,Chiba S,Terashima H,Nakao T

更新日期：1985-10-01 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:Clostridium difficile is a major cause of antibiotic-associated gastrointestinal illness. Recently, an increased incidence of hospital-acquired infections with severe outcomes has been reported in North America and Europe. Current molecular-typing methods for detection of outbreaks and nosocomial transmission are labo...

journal_title：Journal of clinical microbiology

authors： Marsh JW,O'Leary MM,Shutt KA,Pasculle AW,Johnson S,Gerding DN,Muto CA,Harrison LH

更新日期：2006-07-01 00:00:00

abstract：:Enterococcus cecorum (formerly Streptococcus cecorum), originally isolated from poultry intestines, has rarely been encountered in human diseases. A 60-year-old man with liver cirrhosis and hepatocellular carcinoma developed peritonitis on the seventh day of his hospitalization. Cultures of one blood sample and one as...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Chen YC,Yang PC,Ho SW,Luh KT

更新日期：2000-06-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:Several species within the Burkholderia cepacia complex (BCC) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (CF). BCC infection is typically associated with a poor clinical prognosis and decreased survival. These factors, combined with the existence of highly transmissible epidem...

journal_title：Journal of clinical microbiology

authors： Brown AR,Govan JR

更新日期：2007-06-01 00:00:00

abstract：:Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease. ...

journal_title：Journal of clinical microbiology

authors： McCarthy LR,Carlson JR

更新日期：1981-12-01 00:00:00

abstract：:Several biochemical test systems were studied for their potential usefulness for the examination of strains of Campylobacter species. Most (81%) of the C. jejuni strains hydrolyzed sodium hippurate, but strains of C. fetus, C. sputorum, and C. fecalis did not. Some (46%) of the C. jejuni strains and all of the C. sput...

journal_title：Journal of clinical microbiology

authors： Hébert GA,Hollis DG,Weaver RE,Lambert MA,Blaser MJ,Moss CW

更新日期：1982-06-01 00:00:00

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:The epidemiology of new acquisition of antibiotic-resistant organisms (AROs) in community-based skilled nursing facilities (SNFs) is not well studied. To define the incidence, persistence of, and time to new colonization with methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), a...

journal_title：Journal of clinical microbiology

authors： Fisch J,Lansing B,Wang L,Symons K,Cherian K,McNamara S,Mody L

更新日期：2012-05-01 00:00:00

abstract：:Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. Howev...

journal_title：Journal of clinical microbiology

authors： McLean K,Rosenthal CA,Sengupta D,Owens J,Cookson BT,Hoffman NG,Salipante SJ

更新日期：2019-10-23 00:00:00

abstract：:Sixty-three clinical isolates of charcoal-black-pigmented, gram-positive coryneform rods were received for identification by the Centers for Disease Control and Prevention (CDC) and were provisionally designated CDC fermentative coryneform group 4 (FCG4). Forty-five of these were characterized by morphological, physio...

journal_title：Journal of clinical microbiology

authors： Daneshvar MI,Hollis DG,Weyant RS,Jordan JG,MacGregor JP,Morey RE,Whitney AM,Brenner DJ,Steigerwalt AG,Helsel LO,Raney PM,Patel JB,Levett PN,Brown JM

更新日期：2004-09-01 00:00:00

abstract：:The synthetic anticoagulant sodium amylosulfate (SAS) at concentrations of 125 to 2,000 microgram/ml failed to completely neutralize the bactericidal activity of 80 and 50% (by volume) fresh human serum. Furthermore, SAS failed to inhibit the alternative pathway of complement activation in 80% (by volume) fresh human ...

journal_title：Journal of clinical microbiology

authors： Traub WH

更新日期：1977-08-01 00:00:00

abstract：:The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal gr...

journal_title：Journal of clinical microbiology

authors： Murray PR,Wold AD,Schreck CA,Washington JA II

更新日期：1976-07-01 00:00:00

abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high case fatality risk and is caused by the SFTS virus (SFTSV). A retrospective study conducted after the first identification of an SFTS patient in Japan revealed that SFTS is endemic to the region, and the virus exists indig...

journal_title：Journal of clinical microbiology

authors： Yoshikawa T,Fukushi S,Tani H,Fukuma A,Taniguchi S,Toda S,Shimazu Y,Yano K,Morimitsu T,Ando K,Yoshikawa A,Kan M,Kato N,Motoya T,Kuzuguchi T,Nishino Y,Osako H,Yumisashi T,Kida K,Suzuki F,Takimoto H,Kitamoto H,Ma

更新日期：2014-09-01 00:00:00

abstract：:We assessed the genetic and the antigenic variability within the env gene of peripheral blood human immunodeficiency virus (HIV) type 1 (HIV-1) group O populations during the natural course of a female heterosexual infection. Our data revealed the existence of a significant increase in amino acid sequence variability ...

journal_title：Journal of clinical microbiology

authors： Andréoletti L,Réveil B,Moret H,Brodard V,Philbert F,Tabary T,Cohen JH

更新日期：2007-04-01 00:00:00

abstract：:The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...

journal_title：Journal of clinical microbiology

authors： Saribasak H,Salih BA,Yamaoka Y,Sander E

更新日期：2004-04-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:Many diseases with unknown etiology may be caused by unidentified viruses. Sequence-independent amplification revealed a new astrovirus, similar to VA1, in a 4-year-old male diagnosed with celiac disease. This expands the geographic range of this virus to include Europe and may associate astrovirus infection with the ...

journal_title：Journal of clinical microbiology

authors： Smits SL,van Leeuwen M,van der Eijk AA,Fraaij PL,Escher JC,Simon JH,Osterhaus AD

更新日期：2010-09-01 00:00:00

abstract：:Trichomonas vaginalis is the most prevalent nonviral sexually transmitted infection worldwide, and improved diagnostic methods are critical for controlling this pathogen. Diagnostic assays that can be used in conjunction with routine chlamydia/gonorrhea nucleic acid-based screening are likely to have the most impact o...

journal_title：Journal of clinical microbiology

authors： Van Der Pol B,Williams JA,Taylor SN,Cammarata CL,Rivers CA,Body BA,Nye M,Fuller D,Schwebke JR,Barnes M,Gaydos CA

更新日期：2014-03-01 00:00:00

abstract：:A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA resu...

journal_title：Journal of clinical microbiology

authors： Sefers SE,Raymer AK,Kilby JT,Persing DH,Tang YW

更新日期：2009-09-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:The sensitivities of MRC-5 and mink lung (ML) cells in centrifugation culture were compared simultaneously for the detection of cytomegalovirus (CMV) IE antigen (immediate-early antigen) from clinical specimens. Of 413 samples assayed, 51 (12%) were positive for CMV by both centrifugation and standard cell culture. At...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Meyers JD

更新日期：1992-04-01 00:00:00

abstract：:Ehrlichia risticii causes an acute infectious disease in horses called Potomac horse fever. To investigate the biological diversity of E. risticii organisms, nine E. risticii isolates derived from the peripheral blood monocytes of clinically sick horses in Ohio and Kentucky during the summers of 1991 and 1993 were com...

journal_title：Journal of clinical microbiology

authors： Chaichanasiriwithaya W,Rikihisa Y,Yamamoto S,Reed S,Crawford TB,Perryman LE,Palmer GH

更新日期：1994-12-01 00:00:00

abstract：:The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

journal_title：Journal of clinical microbiology

authors： Welch WD,Southern PM Jr,Schneider NR

更新日期：1986-11-01 00:00:00

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

更新日期：2002-10-01 00:00:00

abstract：:Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

journal_title：Journal of clinical microbiology

authors： Anderson MJ,Gull K,Denning DW

更新日期：1996-01-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is an opportunistic infection with airborne transmission and remains a major cause of respiratory illness among immunocompromised individuals. In recent years, several outbreaks of PCP, occurring mostly in kidney transplant recipients, have been reported. Currently, multilocus se...

journal_title：Journal of clinical microbiology

authors： Maitte C,Leterrier M,Le Pape P,Miegeville M,Morio F

更新日期：2013-09-01 00:00:00