Aerococcus-like organism, a newly recognized potential urinary tract pathogen.

abstract：:The correlation of β-glucan (BG) levels with clinical outcomes in invasive candidiasis (IC) remains unknown. Patients with proven IC were followed prospectively from diagnosis to outcome with twice-weekly serum BG sampling. Correlation of BG with clinical outcome was assessed in each patient. BG levels tend to decreas...

journal_title：Journal of clinical microbiology

authors： Sims CR,Jaijakul S,Mohr J,Rodriguez J,Finkelman M,Ostrosky-Zeichner L

更新日期：2012-06-01 00:00:00

abstract：:During an outbreak of diarrheal illness among residents of a trailer park in rural Vermont, 37 (30%) of 122 residents met the case definition of outbreak-related giardiasis. Convalescent-phase sera from 24 residents and 20 nonresident control subjects were tested by enzyme-linked immunosorbent assay for immunoglobulin...

journal_title：Journal of clinical microbiology

authors： Birkhead G,Janoff EN,Vogt RL,Smith PD

更新日期：1989-08-01 00:00:00

abstract：:The dimorphic fungus, Penicillium marneffei, is an emerging opportunistic pathogen endemic in Southeast Asia, especially for those with impaired cellular immunity such as human immunodeficiency virus-infected persons. A discriminatory and reproducible method based on the analysis of nucleotide sequences would facilita...

journal_title：Journal of clinical microbiology

authors： Lasker BA

更新日期：2006-09-01 00:00:00

abstract：:The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

journal_title：Journal of clinical microbiology

authors： Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

更新日期：2018-11-27 00:00:00

abstract：:Better detection of minority human immunodeficiency virus type 1 (HIV-1) populations containing gene mutations may improve the usefulness of antiretroviral resistance testing for clinical management. Molecular cloning of HIV-1 PCR products which might improve minority detection can be slow and difficult, and commercia...

journal_title：Journal of clinical microbiology

authors： Martinez-Picado J,Sutton L,De Pasquale MP,Savara AV,D'Aquila RT

更新日期：1999-09-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:Rapid and accurate identification of mycobacterial species is essential for patient management. We describe the use of the Invader assay in conjunction with the BACTEC MGIT 960 system that together provide an efficient procedure for clinical use. This assay discriminates single-base differences (e.g., genotyping singl...

journal_title：Journal of clinical microbiology

authors： Ichimura S,Nagano M,Ito N,Shimojima M,Egashira T,Miyamoto C,Ohkusu K,Ezaki T

更新日期：2007-10-01 00:00:00

abstract：:The studies reported here were undertaken to assess the ability of the outer membrane proteins (OMPs) of Salmonella typhi to induce a humoral immune response in humans with typhoid fever. OMPs were isolated with the nonionic detergent Triton X-100 and were found to be contaminated with approximately 4% lipopolysacchar...

journal_title：Journal of clinical microbiology

authors： Ortiz V,Isibasi A,García-Ortigoza E,Kumate J

更新日期：1989-07-01 00:00:00

abstract：:Timely diagnosis of respiratory syncytial virus (RSV) infection is critical for appropriate treatment of lower respiratory infection in young children. To facilitate diagnosis, we developed a rapid, specific, and sensitive TaqMan PCR method for detection of RSV A and RSV B. Two sets of primer-probe pairs were selected...

journal_title：Journal of clinical microbiology

authors： Hu A,Colella M,Tam JS,Rappaport R,Cheng SM

更新日期：2003-01-01 00:00:00

abstract：:Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted feve...

journal_title：Journal of clinical microbiology

authors： Stenos J,Ross B,Feng HM,Crocquet-Valdes P,Walker D

更新日期：1997-01-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:We developed a strategy to determine the clinical impact associated with errors in clinical microbiology testing. Over a 9-month period, we used a sequential three-stage method to prospectively evaluate 480 consecutive corrected microbiology laboratory reports. The three stages were physician review of the corrected r...

journal_title：Journal of clinical microbiology

authors： Yuan S,Astion ML,Schapiro J,Limaye AP

更新日期：2005-05-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has proved to be a useful diagnostic method for identifying conventional bacteria. In the case of mycobacteria, a good protein extraction protocol is essential in order to obtain reliable identification results. To date, no suc...

journal_title：Journal of clinical microbiology

authors： Rodriguez-Temporal D,Perez-Risco D,Struzka EA,Mas M,Alcaide F

更新日期：2018-03-26 00:00:00

abstract：:Transfusion-transmissible infections among 808 blood donors in Ghana were investigated in 1999. Antibody seroprevalences of 3.8, 0.7, 8.4, and 13.5%, respectively, for human immunodeficiency virus, human T-cell lymphotrophic virus type 1, hepatitis C virus (HCV), and Treponema pallidum were obtained. The seroprevalenc...

journal_title：Journal of clinical microbiology

authors： Ampofo W,Nii-Trebi N,Ansah J,Abe K,Naito H,Aidoo S,Nuvor V,Brandful J,Yamamoto N,Ofori-Adjei D,Ishikawa K

更新日期：2002-09-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in paucibacillary specimens that are negative for acid-fast bacilli using smear microscopy. Xpert testing includes dilution with sample reagent, and when proces...

journal_title：Journal of clinical microbiology

authors： Dharan NJ,Amisano D,Mboowa G,Ssengooba W,Blakemore R,Kubiak RW,Armstrong DT,Jones M,Manabe YC,Joloba ML,Ellner JJ,Dorman SE,Alland D

更新日期：2015-04-01 00:00:00

abstract：:A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.0...

journal_title：Journal of clinical microbiology

authors： Jaulhac B,Reyrolle M,Sodahlon YK,Jarraud S,Kubina M,Monteil H,Piémont Y,Etienne J

更新日期：1998-07-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque siz...

journal_title：Journal of clinical microbiology

authors： Khan MW,Gallagher M,Bucher D,Cerini CP,Kilbourne ED

更新日期：1982-07-01 00:00:00

abstract：:Iodophor and alcohol pledgets were compared with the Medi-Flex Prep Kit II for skin disinfection before venipuncture. Of 12,367 blood cultures collected, 6,362 were done with conventional pledgets and 6, 005 were done with Medi-Flex kits. Contamination occurred in 351 of 6,362 blood cultures (5.5%; range, 3.7 to 8.1%)...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Mirrett S,Reimer LG,Fernando C,Meredith FT,Reller LB

更新日期：2000-12-01 00:00:00

abstract：:We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this mo...

journal_title：Journal of clinical microbiology

authors： Motoyama AB,Venancio EJ,Brandão GO,Petrofeza-Silva S,Pereira IS,Soares CM,Felipe MS

更新日期：2000-08-01 00:00:00

abstract：:Mycobacterium tuberculosis complex, Mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. Considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high...

journal_title：Journal of clinical microbiology

authors： Sevilla IA,Molina E,Elguezabal N,Pérez V,Garrido JM,Juste RA

更新日期：2015-03-01 00:00:00

abstract：:The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...

journal_title：Journal of clinical microbiology

authors： Wells CL,Field CR

更新日期：1976-12-01 00:00:00

abstract：:Two rapid diagnostic tests for Chlamydia trachomatis (Microtrak, Syva Co., Palo Alto, Calif.; and Chlamydiazyme, Abbott Laboratories, North Chicago, Ill.) were evaluated in comparison with growth of the organism in tissue culture for 2,030 urogenital specimens from men and women. Neither test performed as well as cult...

journal_title：Journal of clinical microbiology

authors： Hipp SS,Han Y,Murphy D

更新日期：1987-10-01 00:00:00

abstract：:This study describes the course of an OXA-48-producing Enterobacteriaceae (OPE) outbreak that started in March 2012 in a neonatal intensive care unit (NICU) in Jerusalem, Israel. During the peak of the outbreak (January to August 2012), there were 49 patients who had proven or suspected acquisition of OPE in the NICU,...

journal_title：Journal of clinical microbiology

authors： Adler A,Solter E,Masarwa S,Miller-Roll T,Abu-Libdeh B,Khammash H,Najem K,Dekadek S,Stein-Zamir C,Nubani N,Kunbar A,Assous MV,Carmeli Y,Schwaber MJ

更新日期：2013-09-01 00:00:00

abstract：:To evaluate the ability of the Amplicor MTB Assay to detect Mycobacterium tuberculosis complex (MTBC) organisms in BACTEC 12B broth cultures, 249 cultures with a growth index (GI) of > or = 20 from 160 patients were tested retrospectively. Specimens were processed by standard methods, and then BACTEC 12B vials and Mid...

journal_title：Journal of clinical microbiology

authors： Smith MB,Bergmann JS,Woods GL

更新日期：1997-04-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

journal_title：Journal of clinical microbiology

authors： Seppänen H

更新日期：1990-04-01 00:00:00

abstract：:A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregati...

journal_title：Journal of clinical microbiology

authors： Toma C,Lu Y,Higa N,Nakasone N,Chinen I,Baschkier A,Rivas M,Iwanaga M

更新日期：2003-06-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00