Prevalence of blood-borne infectious diseases in blood donors in Ghana.

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:The present study describes the use of the automated BACTEC 9240 blood culture system, the Serum Separator Tube (SST), and the BD PHOENIX Automated Microbiology System in combination for the direct identification and antimicrobial susceptibility testing (AST) of gram-negative rods (GNRs) from positive blood cultures (...

journal_title：Journal of clinical microbiology

authors： Funke G,Funke-Kissling P

更新日期：2004-04-01 00:00:00

abstract：:An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the result...

journal_title：Journal of clinical microbiology

authors： Arcavi M,Orfus G,Griemberg G

更新日期：1997-06-01 00:00:00

abstract：:A total of 1,013 isolates of Enterobacteriaceae were identified in parallel by the Repliscan (Cathra International, Ontario, Canada) and API 20E (Analytab Products, Plainview, N.Y.) systems. There was a 62% agreement at the genus level between the two systems. Of the 38% discrepant results, Repliscan classified 22% as...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Quall CO,Fox JM

更新日期：1981-01-01 00:00:00

abstract：:Antibodies to Japanese encephalitis virus (JEV) nonstructural 1 (NS1) protein constitute a marker of natural JEV infection among populations vaccinated with inactivated JE vaccine. In Japan, with few recent human JE cases, the natural infection rate is critical to evaluate the necessity of continuing JE vaccination. A...

journal_title：Journal of clinical microbiology

authors： Konishi E,Shoda M,Ajiro N,Kondo T

更新日期：2004-11-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:Herpesviruses isolated from either domesticated or wild carnivores should be immunologically compared with known viruses of this group which could have been included in the diet of the animal before being considered to be previously undescribed herpesviruses native to the carnivore in question. ...

journal_title：Journal of clinical microbiology

authors： Porter DD,Larsen AE,Cox NA

更新日期：1975-01-01 00:00:00

abstract：:Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

更新日期：2009-10-01 00:00:00

abstract：:A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h. ...

journal_title：Journal of clinical microbiology

authors： Clemons KV,Gadberry JL

更新日期：1982-04-01 00:00:00

abstract：:We have evaluated the diagnostic utility of six antigenic regions of the Toxoplasma gondii MIC2, MIC3, M2AP, GRA3, GRA7, and SAG1 gene products, assembled in recombinant chimeric antigens by genetic engineering, in order to replace the soluble, whole-cell tachyzoite extract in serological assays. Serum samples from 10...

journal_title：Journal of clinical microbiology

authors： Beghetto E,Spadoni A,Bruno L,Buffolano W,Gargano N

更新日期：2006-06-01 00:00:00

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

journal_title：Journal of clinical microbiology

authors： Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

更新日期：2013-02-01 00:00:00

abstract：:Patients with atrophic corpus gastritis and elevated Helicobacter pylori antibody titers but 13C-urea breath test (13C-UBT) and histology results negative for H. pylori were randomized into eradication therapy or follow-up only. Antibody levels decreased significantly in six out of seven patients in the eradication gr...

journal_title：Journal of clinical microbiology

authors： Kokkola A,Rautelin H,Puolakkainen P,Sipponen P,Färkkilä M,Haapiainen R,Kosunen TU

更新日期：1998-06-01 00:00:00

abstract：:A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

journal_title：Journal of clinical microbiology

authors： Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

更新日期：2004-01-01 00:00:00

abstract：:A radioimmunoassay for determination of human immunoglobulin G, A, and M class antibodies to Histoplasma capsulatum is reported. Yeast-phase antigen from H. capsulatum was adsorbed onto polyvinyl chloride microtiter plates as the solid phase. Human antibodies were allowed to react with this antigen, followed by additi...

journal_title：Journal of clinical microbiology

authors： Sprouse RF,Caldwell CW,Everett ED

更新日期：1981-01-01 00:00:00

abstract：:A new blood lysis-filtration culture technique revealed a high incidence of Staphylococcus epidermidis in the blood of patients and of healthy people. Of 2,004 blood cultures from patients, the blood lysis method grew S. epidermidis in 233 (11.6%), whereas a conventional two-bottle culture system grew this organism in...

journal_title：Journal of clinical microbiology

authors： Zierdt CH

更新日期：1983-04-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...

journal_title：Journal of clinical microbiology

authors： Mankikar SD,Middleton PJ,Petric M

更新日期：1979-08-01 00:00:00

abstract：:Two in a group of five naturally seropositive donkey stallions were found to shed equine arteritis virus (EAV) in their semen as demonstrated by virus isolation. Direct intramuscular inoculation of sonicated semen from one virus-shedding stallion (S3) caused clinical disease in two donkeys from which virus was recover...

journal_title：Journal of clinical microbiology

authors： Paweska JT,Volkmann DH,Barnard BJ,Chirnside ED

更新日期：1995-12-01 00:00:00

abstract：:Vibrio cholerae O139 synonym Bengal recently caused large epidemics of cholera-like disease in Bangladesh and India. We compared the restriction fragment length polymorphisms of ctxA and rRNA genes (ribotypes) in 27 isolates of V. cholerae O139 from patients in Bangladesh and India with those of 48 isolates of V. chol...

journal_title：Journal of clinical microbiology

authors： Faruque SM,Abdul Alim AR,Roy SK,Khan F,Nair GB,Sack RB,Albert MJ

更新日期：1994-04-01 00:00:00

abstract：:Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

journal_title：Journal of clinical microbiology

authors： Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

更新日期：2011-11-01 00:00:00

abstract：:Four fluorescein isothiocyanate-labelled monoclonal antibody typing reagents were compared for intensity of fluorescence and sensitivity in confirming herpes simplex virus (HSV) in cell culture. A total of 125 (50 HSV type 1 [HSV-1] and 75 HSV-2) specimens positive for HSV were concurrently stained with type-specific ...

journal_title：Journal of clinical microbiology

authors： Johnston SL,Wellens K

更新日期：1992-07-01 00:00:00

abstract：:A female patient suffered a typical attack of typhoid fever due to a lactose-negative, fully susceptible Salmonella typhi strain. During convalescence she became febrile, and a lactose-fermenting S. typhi strain resistant to ampicillin, chloramphenicol, tetracycline, and trimethoprim was isolated from blood culture. T...

journal_title：Journal of clinical microbiology

authors： Cohen SL,Wylie BA,Sooka A,Koornhof HJ

更新日期：1987-08-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:A 1994 outbreak of acute hemorrhagic conjunctivitis in Israel was caused by an enterovirus 70 strain that was distinct from previously reported strains. Characterization was by electron microscopy (eye washes), reverse transcription-PCR (RT-PCR; eyewash, specimens, eye swabs, and tears), and sequence analysis of RT-PC...

journal_title：Journal of clinical microbiology

authors： Shulman LM,Manor Y,Azar R,Handsher R,Vonsover A,Mendelson E,Rothman S,Hassin D,Halmut T,Abramovitz B,Varsano N

更新日期：1997-08-01 00:00:00

abstract：:Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gen...

journal_title：Journal of clinical microbiology

authors： Nethercott C,Mabbett AN,Totsika M,Peters P,Ortiz JC,Nimmo GR,Coombs GW,Walker MJ,Schembri MA

更新日期：2013-07-01 00:00:00

abstract：:In an initial experiment, culture-grown Borrelia burgdorferi was added to freshly collected uninfected human blood. This in vitro study demonstrated that more spirochetes were distributed into the plasma than into the serum fraction. In a subsequent clinical study, B. burgdorferi was recovered from plasma cultures of ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Bittker S,Cooper D,Nowakowski J,Nadelman RB,Pavia C

更新日期：2000-04-01 00:00:00

abstract：:In this study we used LightCycler PCR amplification and product detection by fluorescence resonance energy transfer probes to identify mycobacteria and differentiate between Mycobacterium tuberculosis complex, Mycobacterium avium, and other nontuberculous mycobacteria. Targeting the 16S rRNA gene, three different prob...

journal_title：Journal of clinical microbiology

authors： Lachnik J,Ackermann B,Bohrssen A,Maass S,Diephaus C,Puncken A,Stermann M,Bange FC

更新日期：2002-09-01 00:00:00

abstract：:A total of 141 independent strains of Pseudomonas aeruginosa with different heterogeneities in the exo gene (exoS, exoT, exoU, and exoY) background were examined for their pathogenic roles. Results indicated that the exoU gene is the major contributor to cytotoxicity in Madin-Darby canine kidney cells but is not relat...

journal_title：Journal of clinical microbiology

authors： Lin HH,Huang SP,Teng HC,Ji DD,Chen YS,Chen YL

更新日期：2006-12-01 00:00:00

abstract：:A strain of Escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (O and K antigens) to the standard strain of E. coli O group 29 and was positive in the Serény test for invasiveness, which suggests that it can cause a Shigella-like illness. ...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Reis MH,Almeida RG,Trabulsi LR

更新日期：1979-02-01 00:00:00