Increased indole detection for Pasteurella multocida.

abstract：:We describe enzyme immunoassays that use synthetic oligopeptides to discriminate serologically between human T-cell lymphotropic virus type I and II (HTLV-I and HTLV-II) infections. The peptides represented 20-amino acid segments between residues 111 and 130 (MA1) and residues 116 and 135 (MA2) of the p19 gag proteins...

journal_title：Journal of clinical microbiology

authors： Bonis J,Baillou A,Barin F,Verdier M,Janvier B,Denis F

更新日期：1993-06-01 00:00:00

abstract：:While testing the in vitro activities of 14 antimicrobial agents against 107 methicillin-susceptible Staphylococcus aureus (MSSA) and 250 methicillin-resistant S. aureus (MRSA) isolates collected in The Netherlands, we found to our surprise that 19 (7.6%) MRSA isolates were suspected of having reduced susceptibilities...

journal_title：Journal of clinical microbiology

authors： Van Griethuysen A,Van 't Veen A,Buiting A,Walsh T,Kluytmans J

更新日期：2003-06-01 00:00:00

abstract：:The lack of a quick, simple, and inexpensive diagnostic test has limited the ability of public health officials to rapidly assess and control outbreaks of Giardia lamblia in child day-care centers. We evaluated the performance of a commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of a...

journal_title：Journal of clinical microbiology

authors： Addiss DG,Mathews HM,Stewart JM,Wahlquist SP,Williams RM,Finton RJ,Spencer HC,Juranek DD

更新日期：1991-06-01 00:00:00

abstract：:The performance of the Amplified Mycobacterium Tuberculosis Direct (AMTD) test (Gen-Probe Inc., San Diego, Calif.) was assessed in a large tertiary care mycobacteriology laboratory. Both acid-fast smear-positive and smear-negative respiratory and nonrespiratory clinical specimens were analyzed. From February 1998 to 4...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Miller DR,Schneider PS,Roberts GD

更新日期：2002-05-01 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system for the recovery of Mycobacterium tuberculosis from pleural fluid are not statistically different than those of the Myco/F lytic liquid culture system. The time to positivity is shorter in the MGIT system (12.7 versus 2...

journal_title：Journal of clinical microbiology

authors： Harausz E,Lusiba JK,Nsereko M,Johnson JL,Toossi Z,Ogwang S,Boom WH,Joloba ML,Tuberculosis Research Unit (TBRU).

更新日期：2015-04-01 00:00:00

abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

journal_title：Journal of clinical microbiology

authors： Martinez LR,Garcia-Rivera J,Casadevall A

更新日期：2001-09-01 00:00:00

abstract：:Between 1998 and 2001, tissues from four captive white-tailed deer were observed to have histologic lesions of systemic lymphocytic vasculitis. These lesions suggested malignant catarrhal fever, although epizootic hemorrhagic disease and bluetongue were included in the differential diagnosis. Initial diagnostic effort...

journal_title：Journal of clinical microbiology

authors： Kleiboeker SB,Miller MA,Schommer SK,Ramos-Vara JA,Boucher M,Turnquist SE

更新日期：2002-04-01 00:00:00

abstract：:A previously described sequence-based epidemiological typing method for clinical and environmental isolates of Legionella pneumophila serogroup 1 was extended by the investigation of three additional gene targets and modification of one of the previous targets. Excellent typeability, reproducibility, and epidemiologic...

journal_title：Journal of clinical microbiology

authors： Gaia V,Fry NK,Afshar B,Lück PC,Meugnier H,Etienne J,Peduzzi R,Harrison TG

更新日期：2005-05-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) against herpes simplex virus type 1 and 2 (HSV-1 and -2, respectively) nuclear antigens were used to identify cells infected with HSV-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of MRC-5 cell monolayers. The results indicate th...

journal_title：Journal of clinical microbiology

authors： McSharry JJ,Costantino R,McSharry MB,Venezia RA,Lehman JM

更新日期：1990-08-01 00:00:00

abstract：:McCoy cells treated with cycloheximide, iododeoxyuridine, and DEAE-dextran and untreated McCoy cells were inoculated with two stock strains of Chlamydia trachomatis and with 231 urethral specimens from men, 53 (23%) of which contained C. trachomatis. Isolation rates, number and quality of inclusions, and quality of th...

journal_title：Journal of clinical microbiology

authors： Benes S,McCormack WM

更新日期：1982-11-01 00:00:00

abstract：:Noroviruses are the leading cause of outbreaks of gastroenteritis in the world. At present, norovirus genogroup II, genotype 4 (GII/4), strains are the most prevalent in many countries. In this study we investigated 55 outbreaks and 35 sporadic cases of norovirus-associated gastroenteritis in food handlers in food-cat...

journal_title：Journal of clinical microbiology

authors： Ozawa K,Oka T,Takeda N,Hansman GS

更新日期：2007-12-01 00:00:00

abstract：:The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivit...

journal_title：Journal of clinical microbiology

authors： Wu TC,Zaza S,Callaway J

更新日期：1989-08-01 00:00:00

abstract：:Monoclonal antibodies were generated from mice immunized with scolex protein antigen of Cysticercus cellulosae. Three monoclonal antibodies specific for cysticercal antigens, which did not show any cross-reactivity with Taenia solium or Taenia saginata antigens, were selected. Each monoclonal antibody coupled to Sepha...

journal_title：Journal of clinical microbiology

authors： Nascimento E,Tavares CA,Lopes JD

更新日期：1987-07-01 00:00:00

abstract：:In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

journal_title：Journal of clinical microbiology

authors： Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

更新日期：1986-09-01 00:00:00

abstract：:The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,McAdam AJ

更新日期：2015-10-01 00:00:00

abstract：:Brain analysis cannot be used for the investigation of active lyssavirus infection in healthy bats because most bat species are protected by conservation directives. Consequently, serology remains the only tool for performing virological studies on natural bat populations; however, the presence of antibodies merely re...

journal_title：Journal of clinical microbiology

authors： Echevarría JE,Avellón A,Juste J,Vera M,Ibáñez C

更新日期：2001-10-01 00:00:00

abstract：:From 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. Eighty-three Veillonella spp. were recovered from 83 children (4%). Most Veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. The infections were polymicrobial in 79 (95%...

journal_title：Journal of clinical microbiology

authors： Brook I

更新日期：1996-05-01 00:00:00

abstract：:Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...

journal_title：Journal of clinical microbiology

authors： Tanner AC,Mathney JM,Kent RL,Chalmers NI,Hughes CV,Loo CY,Pradhan N,Kanasi E,Hwang J,Dahlan MA,Papadopolou E,Dewhirst FE

更新日期：2011-04-01 00:00:00

abstract：:Intravenous and intraarterial catheters were examined microbiologically and morphologically. Bacteria or yeasts were recovered from 38 of the 63 catheters examined, and Staphylococcus epidermidis was present on 29 of the 38 colonized catheters. Examination of unused Teflon catheters ( Jelco ; Surgikos , Inc., Peterbor...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Costerton JW

更新日期：1984-05-01 00:00:00

abstract：:The absence of markers of inflammation in the cerebrospinal fluid (CSF) commonly predicts the absence of herpes simplex virus (HSV) central nervous system (CNS) infection. Consequently, multiple authors have proposed and validated criteria for deferring HSV PCR testing of CSF in immunocompetent hosts with normal CSF w...

journal_title：Journal of clinical microbiology

authors： Hauser RG,Campbell SM,Brandt CA,Wang S

更新日期：2017-05-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed fo...

journal_title：Journal of clinical microbiology

authors： Gerber PF,Xiao CT,Cao D,Meng XJ,Opriessnig T

更新日期：2014-04-01 00:00:00

abstract：:The p1 genes of 60 Mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. An AGT trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. In addition, a novel p1 gene variant named 2c was identified in ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Cao B,Li J,Song S,Tao X,Yin Y,He L,Zhang J

更新日期：2011-08-01 00:00:00

abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...

journal_title：Journal of clinical microbiology

authors： Edwards EA

更新日期：1977-02-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00

abstract：:Blood culture bottles containing antibiotic binding resins are routinely used to minimize artificial sterilization in the presence of antibiotics. However, the resin binding kinetics can differ between antibiotics and concentrations. This study assessed the impact of clinically meaningful peak, midpoint, and trough co...

journal_title：Journal of clinical microbiology

authors： Chen IH,Nicolau DP,Kuti JL

更新日期：2019-09-24 00:00:00

abstract：:A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...

journal_title：Journal of clinical microbiology

authors： Buller RS,Bailey TC,Ettinger NA,Keener M,Langlois T,Miller JP,Storch GA

更新日期：1992-10-01 00:00:00

abstract：:Herpes simplex virus (HSV) causes acute and relapsing symptoms characterized by ulcerative lesions. Laboratory diagnosis of HSV in cutaneous or mucocutaneous lesions has historically been performed with the use of viral cell culture systems; however, these tests are laborious and suffer decreased sensitivity for advan...

journal_title：Journal of clinical microbiology

authors： Faron ML,Ledeboer NA,Patel A,Beqa SH,Yen-Lieberman B,Kohn D,Leber AL,Mayne D,Northern WI,Buchan BW

更新日期：2016-08-01 00:00:00

abstract：:The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A o...

journal_title：Journal of clinical microbiology

authors： Gibb TR,Norwood DA Jr,Woollen N,Henchal EA

更新日期：2001-11-01 00:00:00

abstract：:The conventional phage typing scheme proposed by S. Basu and S. Mukerjee (Experientia 24:299-300, 1968) has been used routinely for identification of the strains at the Vibrio Phage Reference Laboratory since 1968. However, because of limitations of this scheme, a new phage typing scheme using five newly isolated phag...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay DJ,Sarkar BL,Ansari MQ,Chakrabarti BK,Roy MK,Ghosh AN,Pal SC

更新日期：1993-06-01 00:00:00