Discrimination between human T-cell lymphotropic virus type I and II (HTLV-I and HTLV-II) infections by using synthetic peptides representing an immunodominant region of the core protein (p19) of HTLV-I and HTLV-II.

abstract：:Streptococcus pneumoniae is the major cause of bacterial meningitis. We report a case of meningitis due to a mixed infection with two distinct strains of S. pneumoniae: one penicillin-resistant strain of serotype 9V and one penicillin-susceptible strain of serotype 7. The two strains exhibited different pulsed-field g...

journal_title：Journal of clinical microbiology

authors： Chaves F,Campelo C,Sanz F,Otero JR

更新日期：2003-01-01 00:00:00

abstract：:Virology laboratories historically have used direct fluorescent-antibody assay (DFA) and culture to detect six or seven respiratory viruses. Following the discovery of five new human respiratory viruses since 2000, there is an increasing need for diagnostic tests to detect these emerging viruses. We have developed a n...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Merante F,Yaghoubian S,Sinha T,Lisle C,Janeczko R

更新日期：2007-09-01 00:00:00

abstract：:Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorph...

journal_title：Journal of clinical microbiology

authors： Zingg BC,Brown RN,Lane RS,LeFebvre RB

更新日期：1993-12-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase ch...

journal_title：Journal of clinical microbiology

authors： Eyers M,Chapelle S,Van Camp G,Goossens H,De Wachter R

更新日期：1993-12-01 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

journal_title：Journal of clinical microbiology

authors： Chen HI,Hulten K,Clarridge JE 3rd

更新日期：2002-09-01 00:00:00

abstract：:We report the first case of spontaneous intrapartum Atopobium vaginae bacteremia identified by 16S rRNA gene sequencing. The bacterium was misidentified by RapID ANA II, API Rapid ID 32A, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The likely source of bacteremia wa...

journal_title：Journal of clinical microbiology

authors： Chan JF,Lau SK,Curreem SO,To KK,Leung SS,Cheng VC,Yuen KY,Woo PC

更新日期：2012-07-01 00:00:00

abstract：:A uracil-to-cytosine mutation at nucleotide position 472 of oral poliovirus vaccine type 3 (OPV3) contributes to the development of vaccine-associated paralytic poliomyelitis (VAPP). To analyze OPV3 shedding patterns, we previously used the multistep method of mutant analysis by PCR and enzyme cleavage (MAPREC). This ...

journal_title：Journal of clinical microbiology

authors： Gnanashanmugam D,Falkovitz-Halpern MS,Dodge A,Fang M,Wong LJ,Esparza M,Hammon R,Rivas-Merelles EE,Santos JI,Maldonado Y

更新日期：2007-08-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus. DNA was isolat...

journal_title：Journal of clinical microbiology

authors： Huijsdens XW,Linskens RK,Mak M,Meuwissen SG,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2002-12-01 00:00:00

abstract：:We document for the first time an infectious but nonarthritogenic variant of Borrelia burgdorferi. Strain 25015, previously isolated from an Ixodes dammini larva collected in upstate New York, was infectious but failed to produce arthritis or carditis in laboratory rats and mice. By contrast, pathogenic strain N40 inv...

journal_title：Journal of clinical microbiology

authors： Anderson JF,Barthold SW,Magnarelli LA

更新日期：1990-12-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:A protein antigen with an apparent molecular weight (Mr) of 31,000 was isolated from 0.2 M glycine hydrochloride (pH 2.2) extracts of a typical human fecal isolate, Campylobacter jejuni VC74. The protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving w...

journal_title：Journal of clinical microbiology

authors： Dubreuil JD,Kostrzynska M,Logan SM,Harris LA,Austin JW,Trust TJ

更新日期：1990-06-01 00:00:00

abstract：:We explored three genes of attachment (fbe and atlE) and adhesion (ica) in 27 and 10 Staphylococcus epidermidis strains involved in pacemaker-related infections (PMI) and intravascular-catheter-related infections (IVCI), respectively, and in 25 saprophytic strains. The detection rates of fbe and atlE were identical in...

journal_title：Journal of clinical microbiology

authors： Klug D,Wallet F,Kacet S,Courcol RJ

更新日期：2003-07-01 00:00:00

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

journal_title：Journal of clinical microbiology

authors： Townsend EL,Maheswaran SK,Leininger JR,Ames TR

更新日期：1987-02-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells...

journal_title：Journal of clinical microbiology

authors： Pesanti EL,McCarron BA

更新日期：1988-08-01 00:00:00

abstract：:Phenotypic, genotypic, and toxin gene analyses have not yet been done all in one for the Nigerian Staphylococcus aureus population. This study provides a comprehensive overview of the molecular epidemiology and genetic diversity of S. aureus strains at the largest university clinic in Ibadan, Nigeria. From 1,300 patie...

journal_title：Journal of clinical microbiology

authors： Ghebremedhin B,Olugbosi MO,Raji AM,Layer F,Bakare RA,König B,König W

更新日期：2009-09-01 00:00:00

abstract：:In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

journal_title：Journal of clinical microbiology

authors： Vollmer T,Störmer M,Kleesiek K,Dreier J

更新日期：2008-06-01 00:00:00

abstract：:Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab an...

journal_title：Journal of clinical microbiology

authors： Silbert S,Rocchetti TT,Gostnell A,Kubasek C,Widen R

更新日期：2016-06-01 00:00:00

abstract：:Since January 1996, and over a 3-year time span, a significant spread of serotype 6B multidrug-resistant (MDR) pneumococci, susceptible to penicillin and resistant to erythromycin, clindamycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole, was noted in young carriers living in central and southern ...

journal_title：Journal of clinical microbiology

authors： Syrogiannopoulos GA,Bogaert D,Grivea IN,Beratis NG,De Groot R R,Hermans PW

更新日期：2001-02-01 00:00:00

abstract：:Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and prote...

journal_title：Journal of clinical microbiology

authors： Atanassov C,Pezennec L,d'Alayer J,Grollier G,Picard B,Fauchère JL

更新日期：2002-02-01 00:00:00

abstract：:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcera...

journal_title：Journal of clinical microbiology

authors： Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel G

更新日期：2010-10-01 00:00:00

abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...

journal_title：Journal of clinical microbiology

authors： Barnishan J,Ayers LW

更新日期：1979-02-01 00:00:00

abstract：:We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...

journal_title：Journal of clinical microbiology

authors： Peel MM,Hornidge KA,Luppino M,Stacpoole AM,Weaver RE

更新日期：1991-11-01 00:00:00