Norovirus infections in symptomatic and asymptomatic food handlers in Japan.

abstract：:A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Maher LA,Howell AW

更新日期：1990-03-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Sampson-Scherer J

更新日期：1977-03-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:Pseudomonas mendocina has been isolated from soil and water samples. Although it has been recovered from some human clinical samples, its pathogenic role has not yet been documented. We report the first known case of endocarditis in humans due to P. mendocina. ...

journal_title：Journal of clinical microbiology

authors： Aragone MR,Maurizi DM,Clara LO,Navarro Estrada JL,Ascione A

更新日期：1992-06-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distributio...

journal_title：Journal of clinical microbiology

authors： Stokell JR,Khan A,Steck TR

更新日期：2014-07-01 00:00:00

abstract：:The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not...

journal_title：Journal of clinical microbiology

authors： Boom WH,Piper DJ,Ruoff KL,Ferraro MJ

更新日期：1985-11-01 00:00:00

abstract：:The development of diagnostic susceptibility tests for CEM-101, a new fluoroketolide, was addressed by structured studies to determine the optimal disk diffusion test concentration and effects of various testing conditions or supplements and to establish the quality control (QC) ranges for reference broth microdilutio...

journal_title：Journal of clinical microbiology

authors： Jones RN,Ross JE,Rhomberg PR,Quality Control Working Group.

更新日期：2010-04-01 00:00:00

abstract：:In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

journal_title：Journal of clinical microbiology

authors： Harris MC,Deuber C,Polin RA,Nachamkin I

更新日期：1989-10-01 00:00:00

abstract：:Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction prot...

journal_title：Journal of clinical microbiology

authors： Duenngai K,Sithithaworn P,Rudrappa UK,Iddya K,Laha T,Stensvold CR,Strandgaard H,Johansen MV

更新日期：2008-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for blastomycosis was evaluated with 65 human sera from culturally or histologically proven cases of blastomycosis, 53 sera from humans with heterologous infections, and 115 sera from apparently normal people. The diagnostic value of the ELISA was compared with that of the ...

journal_title：Journal of clinical microbiology

authors： Turner S,Kaufman L,Jalbert M

更新日期：1986-02-01 00:00:00

abstract：:Candida auris is a multidrug-resistant yeast that causes a wide spectrum of infections, especially in intensive care settings. We investigated C. auris prevalence among 102 clinical isolates previously identified as Candida haemulonii or Candida famata by the Vitek 2 system. Internal transcribed spacer region (ITS) se...

journal_title：Journal of clinical microbiology

authors： Kathuria S,Singh PK,Sharma C,Prakash A,Masih A,Kumar A,Meis JF,Chowdhary A

更新日期：2015-06-01 00:00:00

abstract：:A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoassay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19. I...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Saint-Antoine P,Olivier C,Voyer H,Kessous-Elbaz A,Berrada F,Bégin P,Giroux L,Viscidi R

更新日期：1991-11-01 00:00:00

abstract：:Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

journal_title：Journal of clinical microbiology

authors： Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

更新日期：2003-06-01 00:00:00

abstract：:A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

journal_title：Journal of clinical microbiology

authors： Costas M,Cookson BD,Talsania HG,Owen RJ

更新日期：1989-11-01 00:00:00

abstract：:Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isol...

journal_title：Journal of clinical microbiology

authors： Shafer RW,Winters MA,Mayers DL,Japour AJ,Kuritzkes DR,Weislow OS,White F,Erice A,Sannerud KJ,Iversen A,Pena F,Dimitrov D,Frenkel LM,Reichelderfer PS

更新日期：1996-07-01 00:00:00

abstract：:This study involved the rapid, direct identification of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Haemophilus influenzae, Enterococcus sp., and Streptococcus agalactiae from positive blood culture bottles (BACTEC, Johnston Laboratories, Inc.) by using the AccuProbe (Gen-Probe, San Diego, Calif...

journal_title：Journal of clinical microbiology

authors： Davis TE,Fuller DD

更新日期：1991-10-01 00:00:00

abstract：:In the context of hematopoietic stem cell transplantation, adenovirus infections are associated with relevant mortality and morbidity. Detection of adenovirus DNA by quantitative PCR is the "gold standard" for these patients. A total of 150 samples, namely, 78 whole-blood, 22 cerebrospinal fluid, 24 digestive biopsy, ...

journal_title：Journal of clinical microbiology

authors： Jeulin H,Salmon A,Bordigoni P,Venard V

更新日期：2010-09-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from ...

journal_title：Journal of clinical microbiology

authors： Summers PL,Dubois DR,Cohen WH,Macarthy PO,Binn LN,Sjogren MH,Snitbhan R,Innis BL,Eckels KH

更新日期：1993-05-01 00:00:00

abstract：:Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acet...

journal_title：Journal of clinical microbiology

authors： Guerrant GO,Moss CW

更新日期：1986-03-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:Conditions were defined for functional covalent coupling of anti-Lassa virus globulins to glutaraldehyde-fixed chicken erythrocytes. Tolylene-2,4-diisocyanate in a reaction mixture containing not more than 0.01 M NaCl produced uniformly good conjugates which were used in reversed passive hemagglutination (RPH) and rev...

journal_title：Journal of clinical microbiology

authors： Goldwasser RA,Elliott LH,Johnson KM

更新日期：1980-06-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00

abstract：:Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the...

journal_title：Journal of clinical microbiology

authors： Blazevic DJ,Stemper JE,Matsen JM

更新日期：1975-02-01 00:00:00

abstract：:Crude extracts of Dolichos biflorus can be coupled to polystyrene particles to yield an agglutination reagent for the detection of group C streptococcal antigen extracts. The reagent is relatively inexpensive and simple to prepare and can be employed for the definitive identification of this beta-hemolytic streptococc...

journal_title：Journal of clinical microbiology

authors： Slifkin M,Gil GM

更新日期：1984-01-01 00:00:00

abstract：:We designed a highly sensitive and specific polymerase chain reaction assay for the detection of Epstein-Barr virus (EBV)-related sequences in B-cell DNA of EBV-seropositive healthy individuals. By using this assay, we were able to amplify at least 10 copies of a plasmid containing the BamHI-W region, which is repeate...

journal_title：Journal of clinical microbiology

authors： Wagner HJ,Bein G,Bitsch A,Kirchner H

更新日期：1992-11-01 00:00:00