Abstract:
:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distribution within sputum, we measured the difference in mean total bacterial abundance and abundance of Burkholderia multivorans between aliquots of DTT-treated sputum samples with and without a mechanical homogenization (MH) step using a high-speed dispersing element. Additionally, we measured the effect of MH on bacterial abundance. We found a significant difference between the mean bacterial abundances in aliquots that were subjected to only DTT treatment and those of the aliquots which included an MH step (all bacteria, P = 0.04; B. multivorans, P = 0.05). There was no significant effect of MH on bacterial abundance in sputum. Although our results are from a single CF patient, they indicate that mechanical homogenization increases the homogeneity of bacteria in sputum.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Stokell JR,Khan A,Steck TRdoi
10.1128/JCM.00487-14subject
Has Abstractpub_date
2014-07-01 00:00:00pages
2340-5issue
7eissn
0095-1137issn
1098-660Xpii
JCM.00487-14journal_volume
52pub_type
杂志文章abstract::An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.12.2779-2785.1990
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.34.5.1235-1248.1996
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.30.4.1045-1048.1992
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pub_type: 杂志文章,多中心研究
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.39.12.4390-4395.2001
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.29.3.611-616.1991
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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更新日期:1996-08-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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journal_title:Journal of clinical microbiology
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