Molecular detection and identification of agents of eumycetoma: detailed report of two cases.

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:The Roche Linear Array (LA) and Innogenetics INNO-LiPA human papillomavirus (HPV) genotyping assays were compared for paraffin-embedded vulval tissues. The LA detected amplifiable DNA in 28 (57%) out of 49 biopsy specimens, 20 (40%) being HPV genotyped, compared to 49 (100%) and 41 (83%), respectively, detected by the...

journal_title：Journal of clinical microbiology

authors： Tan SE,Garland SM,Rumbold AR,Tabrizi SN

更新日期：2010-04-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping r...

journal_title：Journal of clinical microbiology

authors： Moser SA,Mitchell SC,Ruby JD,Momeni S,Osgood RC,Whiddon J,Childers NK

更新日期：2010-02-01 00:00:00

abstract：:A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified...

journal_title：Journal of clinical microbiology

authors： Schoone GJ,Oskam L,Kroon NC,Schallig HD,Omar SA

更新日期：2000-11-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:Fifteen common serotypes representative of group C(1) and C(2)Salmonella were characterized using a single set of phages. ...

journal_title：Journal of clinical microbiology

authors： Gershman M

更新日期：1976-02-01 00:00:00

abstract：:There are no previous reports of human infection due to Acinetobacter baylyi. In this study, we report on six patients with bacteremia due to A. baylyi, based on analysis of the 16S-23S rRNA intergenic spacer and the 16S rRNA gene. All six patients had multiple underlying diseases. The infection was nosocomially acqui...

journal_title：Journal of clinical microbiology

authors： Chen TL,Siu LK,Lee YT,Chen CP,Huang LY,Wu RC,Cho WL,Fung CP

更新日期：2008-09-01 00:00:00

abstract：:During a 2-month period, 62 strains of Haemophilus ducreyi were isolated from 168 genital lesions and 2 lymph node aspirates. Of these strains, 22 were found on both chocolate agar and fetal bovine serum agar supplemented with vancomycin, 29 were found only on chocolate agar, and 9 were found only on fetal bovine seru...

journal_title：Journal of clinical microbiology

authors： Hannah P,Greenwood JR

更新日期：1982-11-01 00:00:00

abstract：:A previously established multiplex PCR that identifies to the species level Acinetobacter baumannii and Acinetobacter genomic species 13TU (GS13TU) was expanded to include Acinetobacter calcoaceticus and Acinetobacter genomic species 3. ...

journal_title：Journal of clinical microbiology

authors： Higgins PG,Lehmann M,Wisplinghoff H,Seifert H

更新日期：2010-12-01 00:00:00

abstract：:Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Cl...

journal_title：Journal of clinical microbiology

authors： Baldellon C,Mégraud F

更新日期：1985-03-01 00:00:00

abstract：:A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

journal_title：Journal of clinical microbiology

authors： Fakhr MK,Logue CM

更新日期：2007-10-01 00:00:00

abstract：:Helicobacter pylori strains possessing the cag pathogenicity island (PaI) are associated with the development of gastroduodenal diseases, including gastric cancer. cag PaI products induce the secretion of interleukin-8 (IL-8) from epithelial cells and facilitate the translocation of CagA into the cell cytosol. In East...

journal_title：Journal of clinical microbiology

authors： Zhang Y,Argent RH,Letley DP,Thomas RJ,Atherton JC

更新日期：2005-02-01 00:00:00

abstract：:The rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the ...

journal_title：Journal of clinical microbiology

authors： Ignatyeva O,Kontsevaya I,Kovalyov A,Balabanova Y,Nikolayevskyy V,Toit K,Dragan A,Maxim D,Mironova S,Kummik T,Muntean I,Koshkarova E,Drobniewski F

更新日期：2012-05-01 00:00:00

abstract：:In January 1991, an outbreak of cholera started in Peru and spread throughout most of Latin America within 8 months. As of March 1992, over 450,000 cases and approximately 4,000 deaths have been reported to the Pan American Health Organization. The causative organism is toxigenic Vibrio cholerae O1 of the El Tor bioty...

journal_title：Journal of clinical microbiology

authors： Fields PI,Popovic T,Wachsmuth K,Olsvik O

更新日期：1992-08-01 00:00:00

abstract：:Lyme disease prevails as the most commonly transmitted tick-borne infection in the United States, and serologic evaluation for antibodies to Borrelia burgdorferi remains the recommended modality for diagnosis. This review presents a brief historical perspective on the evolution of serologic assays for Lyme disease and...

journal_title：Journal of clinical microbiology

authors： Theel ES

更新日期：2016-05-01 00:00:00

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:We investigated the antibody response in women with postpartum fever from whom Ureaplasma urealyticum had been isolated from the bloodstream. Acute- and convalescent-phase sera were tested for immunoglobulin G to the polypeptides of five serovars (1, 2, 3, 4, and 8), representing the two genomic clusters of U. urealyt...

journal_title：Journal of clinical microbiology

authors： Lee GY,Kenny GE

更新日期：1987-10-01 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00

abstract：:Recovery of aerobic and anaerobic bacteria from clinical specimens maintained in the Copan Venturi Transystem and the Becton Dickinson Port-a-Cul transport was assessed. Of 54 anaerobes, 53 were recovered after 4 h, and 52 were recovered after 24 h, from both systems. After 48 h, 45 and 50 were recovered from the two ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Hudspeth MK,Goldstein EJ

更新日期：2000-02-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00

abstract：:The in vitro activities of 17 antifungal drugs against a panel of 20 dermatophytes comprising 6 different species were determined using a microdilution assay according to the NCCLS M38-P method with some modifications. Terbinafine was the most potent systemic drug while tolnaftate and amorolfine were the most active t...

journal_title：Journal of clinical microbiology

authors： Favre B,Hofbauer B,Hildering KS,Ryder NS

更新日期：2003-10-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:During a previous survey, five extended-spectrum beta-lactamase (ESBL)-producing enterobacteria (ESBLE) (two Enterobacter aerogenes isolates expressing TEM-24 b, two Escherichia coli isolates expressing TEM-21 or TEM-24 b, and one Klebsiella pneumoniae isolate expressing SHV-4/TEM-15) responsible for urinary tract inf...

journal_title：Journal of clinical microbiology

authors： Arpin C,Dubois V,Maugein J,Jullin J,Dutilh B,Brochet JP,Larribet G,Fischer I,Quentin C

更新日期：2005-10-01 00:00:00

abstract：:The emergence of a clinically daptomycin-resistant Staphylococcus aureus isolate occurred during treatment of methicillin-resistant S. aureus bacteremia and probable vertebral osteomyelitis. The breakthrough isolate was indistinguishable from pretreatment daptomycin-susceptible isolates by pulsed-field gel electrophor...

journal_title：Journal of clinical microbiology

authors： Marty FM,Yeh WW,Wennersten CB,Venkataraman L,Albano E,Alyea EP,Gold HS,Baden LR,Pillai SK

更新日期：2006-02-01 00:00:00

abstract：:Naturally acquired murine cytomegalovirus (MCMV) infection in laboratory strains of mice induces antibody levels which are generally undetectable by standard techniques; therefore, MCMV has not been included routinely in mouse viral antibody screening programs. The relative sensitivity of three assay systems, the nucl...

journal_title：Journal of clinical microbiology

authors： Anderson CA,Murphy JC,Fox JG

更新日期：1983-10-01 00:00:00

abstract：:A panel (ENVA-1) of well-defined blinded samples containing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase was analyzed by automated DNA sequencing in 23 laboratories worldwide. Drug resistance mutations at codons 41, 215, and 184 were present in the panel samples at different r...

journal_title：Journal of clinical microbiology

authors： Schuurman R,Demeter L,Reichelderfer P,Tijnagel J,de Groot T,Boucher C

更新日期：1999-07-01 00:00:00

abstract：:Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutinati...

journal_title：Journal of clinical microbiology

authors： Kuzuya M,Fujii R,Hamano M,Yamada M,Shinozaki K,Sasagawa A,Hasegawa S,Kawamoto H,Matsumoto K,Kawamoto A,Itagaki A,Funatsumaru S,Urasawa S

更新日期：1998-01-01 00:00:00

abstract：:We describe a simple method for the rapid detection of bovine viral diarrhea virus (BVDV) that uses a one-tube reverse transcription PCR (RT-PCR) and total RNA extracted directly from a variety of bovine specimens, including whole blood and tissues. Reagents for both RT and PCR were combined in a one-tube, single-buff...

journal_title：Journal of clinical microbiology

authors： Hamel AL,Wasylyshen MD,Nayar GP

更新日期：1995-02-01 00:00:00

abstract：:Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonom...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Armfield AY

更新日期：1979-10-01 00:00:00