Sensitive detection of Treponema pallidum by using the polymerase chain reaction.

abstract：:The complement fixation (CF) test is the current reference serologic test for the diagnosis of Mycoplasma pneumoniae infection. However, it is reported to be insensitive and nonspecific, and it is labor intensive. To determine if a faster and more sensitive diagnosis of M. pneumoniae could be obtained, we examined 50 ...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Talkington DF

更新日期：1995-05-01 00:00:00

abstract：:A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior ...

journal_title：Journal of clinical microbiology

authors： Hess G,Avillez F,Lourenco MH,D'Agostino F,Cambie G,Piot P,Vercauteren G,Michl U,Melchior W,Bayer H

更新日期：1994-02-01 00:00:00

abstract：:A total of 244 strains of Aerococcus, Enterococcus, and Streptococcus species were tested by the RapID STR system (Innovative Diagnostic Systems Inc., Atlanta, Ga.) for identification. Strains were identified without additional tests or with additional conventional tests suggested by the IDS compendium manual. Our dat...

journal_title：Journal of clinical microbiology

authors： You MS,Facklam RR

更新日期：1986-10-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

journal_title：Journal of clinical microbiology

authors： Barreau C,Wagener G

更新日期：1990-08-01 00:00:00

abstract：:The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal gr...

journal_title：Journal of clinical microbiology

authors： Murray PR,Wold AD,Schreck CA,Washington JA II

更新日期：1976-07-01 00:00:00

abstract：:We report a case of bacteremia caused by Janibacter melonis, a recently described aerobic actinomycete originally isolated from a spoiled oriental melon. Our patient's blood culture isolate was identified by partial 16S rRNA gene sequencing. This is the first report of the recovery of Janibacter species from humans. ...

journal_title：Journal of clinical microbiology

authors： Elsayed S,Zhang K

更新日期：2005-07-01 00:00:00

abstract：:A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

journal_title：Journal of clinical microbiology

authors： Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

更新日期：1995-05-01 00:00:00

abstract：:Mycobacterium abscessus, which consists of the two subspecies M. abscessus subspecies abscessus and M. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. Here we analyzed 50 M. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (...

journal_title：Journal of clinical microbiology

authors： Rüger K,Hampel A,Billig S,Rücker N,Suerbaum S,Bange FC

更新日期：2014-01-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:Since January 1996, and over a 3-year time span, a significant spread of serotype 6B multidrug-resistant (MDR) pneumococci, susceptible to penicillin and resistant to erythromycin, clindamycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole, was noted in young carriers living in central and southern ...

journal_title：Journal of clinical microbiology

authors： Syrogiannopoulos GA,Bogaert D,Grivea IN,Beratis NG,De Groot R R,Hermans PW

更新日期：2001-02-01 00:00:00

abstract：:The aim of this study was to develop and evaluate multiplex and nested PCR-reverse line blot (RLB) hybridization assays for detection and serovar identification of Chlamydia trachomatis. Two sets of primers targeting the VD2 region of the omp1 gene and one set targeting the cryptic plasmid were designed for use in mul...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Zhou H,Gilbert GL

更新日期：2006-04-01 00:00:00

abstract：:To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

journal_title：Journal of clinical microbiology

authors： Everts RJ,Heneghan JP,Adholla PO,Reller LB

更新日期：2001-01-01 00:00:00

abstract：:A simple and reliable technique was developed for differentiating Helicobacter pylori strains by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified DNAs. Oligonucleotide primer pairs developed to the urease, 48-kDa stress protein (htrA), and 26-kDa antigen-encoding genes wer...

journal_title：Journal of clinical microbiology

authors： Clayton CL,Kleanthous H,Morgan DD,Puckey L,Tabaqchali S

更新日期：1993-06-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:The NCCLS M38-A document does not describe guidelines for testing caspofungin acetate (MK-0991) and other echinocandins against molds. This study evaluated the susceptibilities of 200 isolates of Aspergillus fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus to caspofungin (MICs and minimum effective concentr...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A

更新日期：2003-01-01 00:00:00

abstract：:Penicilliosis, caused by the dimorphic fungus Penicillium marneffei, is an important opportunistic systemic fungal infection affecting immunocompromised individuals living in areas where penicilliosis is endemic. We have demonstrated previously that a urinary enzyme-linked immunosorbent assay (ELISA) with purified rab...

journal_title：Journal of clinical microbiology

authors： Desakorn V,Simpson AJ,Wuthiekanun V,Sahassananda D,Rajanuwong A,Pitisuttithum P,Howe PA,Smith MD,White NJ

更新日期：2002-09-01 00:00:00

abstract：:Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance was due to the production of CTX-M-type extended-spectrum beta-lactamases encoded by similar 12-kb plasmids. By pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Tassios PT,Gazouli M,Tzelepi E,Milch H,Kozlova N,Sidorenko S,Legakis NJ,Tzouvelekis LS

更新日期：1999-11-01 00:00:00

abstract：:Antiserum specific for Salmonella O3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-D- Manp (1----4)-alpha-L- Rhap (1----3)-alpha-D-Galp covalently linked to bovine serum albumin (beta- MRG -BSA). Enzyme immunoassays showed that only lipop...

journal_title：Journal of clinical microbiology

authors： Ekwall E,Norberg T,Svenson SB,Lindberg AA

更新日期：1984-05-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

journal_title：Journal of clinical microbiology

authors： Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

更新日期：1996-11-01 00:00:00

abstract：:Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping have focused ...

journal_title：Journal of clinical microbiology

authors： Batt SL,Charalambous BM,McHugh TD,Martin S,Gillespie SH

更新日期：2005-06-01 00:00:00

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manne...

journal_title：Journal of clinical microbiology

authors： Beck ET,Buchan BW,Riebe KM,Alkins BR,Pancholi P,Granato PA,Ledeboer NA

更新日期：2014-06-01 00:00:00

abstract：:Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform...

journal_title：Journal of clinical microbiology

authors： Anderson TP,Werno AM,Beynon KA,Murdoch DR

更新日期：2003-05-01 00:00:00

abstract：:Mycoplasma pneumoniae is a common cause of community-acquired pneumonia and lower-respiratory-tract infections. Diagnosis has traditionally been obtained by serological diagnosis, but increasingly, molecular techniques have been applied. However, the number of studies actually comparing these assays is limited. The de...

journal_title：Journal of clinical microbiology

authors： Templeton KE,Scheltinga SA,Graffelman AW,Van Schie JM,Crielaard JW,Sillekens P,Van Den Broek PJ,Goossens H,Beersma MF,Claas EC

更新日期：2003-09-01 00:00:00

abstract：:In clinical laboratories, diagnosis of imported malaria is commonly performed by microscopy. However, the volume of specimens is generally low and maintaining proficiency in reading blood smears, particularly at the species level, is challenging in this setting. To address this problem, the Provincial Laboratory for P...

journal_title：Journal of clinical microbiology

authors： Shokoples S,Mukhi SN,Scott AN,Yanow SK

更新日期：2013-06-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:The value of performing routine aerobic subcultures of both vented and unvented blood culture bottles has not been evaluated critically. We studied 4,954 pairs of blood culture bottles consisting of one vented biphasic tryptic soy broth bottle (Roche Diagnostics) and one unvented Thiol broth bottle (Difco Laboratories...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Westfall LM,Murray PR

更新日期：1983-04-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00