Impact of routine real-time PCR testing of imported malaria over 4 years of implementation in a clinical laboratory.

abstract：:Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) re...

journal_title：Journal of clinical microbiology

authors： Zengzhu G,Bracha R,Nuchamowitz Y,Cheng-I W,Mirelman D

更新日期：1999-09-01 00:00:00

abstract：:The Analytab Products, Inc. (API), anaerobic multitest microsystem (MICRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin K1) system and with pre-reduced anaerobically sterilized (PRAS) media as recommended by the Virginia Polytechnic Institute. ...

journal_title：Journal of clinical microbiology

authors： Moore HB,Sutter VL,Finegold SM

更新日期：1975-01-01 00:00:00

abstract：:We conducted a case-control study using the Fungitell assay, the novel Platelia Candida Antigen (Ag) Plus and Candida Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1→3)-β-D-glucan (BDG), mannan antigen with/without anti-mannan antibody, and Cand-Tec Candida antigen...

journal_title：Journal of clinical microbiology

authors： Held J,Kohlberger I,Rappold E,Busse Grawitz A,Häcker G

更新日期：2013-04-01 00:00:00

abstract：:Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

journal_title：Journal of clinical microbiology

authors： Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

更新日期：2017-05-01 00:00:00

abstract：:We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...

journal_title：Journal of clinical microbiology

authors： Dini LA,Frean JA

更新日期：2005-12-01 00:00:00

abstract：:In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized ...

journal_title：Journal of clinical microbiology

authors： Hujer KM,Hujer AM,Endimiani A,Thomson JM,Adams MD,Goglin K,Rather PN,Pennella TT,Massire C,Eshoo MW,Sampath R,Blyn LB,Ecker DJ,Bonomo RA

更新日期：2009-05-01 00:00:00

abstract：:The performance of a human herpesvirus 8 (HHV-8) enzyme immunoassay (EIA) and selective subsequent use of an HHV-8 immunofluorescence assay (IFA) was tested in persons unlikely to be infected with HHV-8 and those who had HHV-8 detected in their saliva. The IFA was performed on samples within a range of EIA optical den...

journal_title：Journal of clinical microbiology

authors： Casper C,Krantz E,Taylor H,Dalessio J,Carrell D,Wald A,Corey L,Ashley R

更新日期：2002-10-01 00:00:00

abstract：:The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...

journal_title：Journal of clinical microbiology

authors： Ebel A,Vanneste L,Cardinaels M,Sablon E,Samson I,De Bosschere K,Hulstaert F,Zrein M

更新日期：2000-01-01 00:00:00

abstract：:Disk diffusion and broth microdilution (BMD) were used to perform clindamycin (CLI) induction testing on 128 selected nonduplicate isolates of Staphylococcus aureus. Disk diffusion testing involved placing CLI and erythromycin (ERY) disks approximately 12 mm apart (measured edge to edge) on a Mueller-Hinton agar plate...

journal_title：Journal of clinical microbiology

authors： Steward CD,Raney PM,Morrell AK,Williams PP,McDougal LK,Jevitt L,McGowan JE Jr,Tenover FC

更新日期：2005-04-01 00:00:00

abstract：:Clinical isolates of group B streptococci from body fluids and mucosal surfaces were examined for production of a trypsin-resistant antigen known as R protein. R protein was extracted with 1% trypsin from cells grown in a semidefined medium. The extracts were tested by immunodiffusion in agarose with a panel of antise...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1989-05-01 00:00:00

abstract：:Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy and those of 8 patients after therapy were examined for Borrelia burgdorferi DNA by PCR. Skin biopsies of 27 patients with dermatological diseases other than Lyme borreliosis and those of 10 healthy persons ...

journal_title：Journal of clinical microbiology

authors： Brettschneider S,Bruckbauer H,Klugbauer N,Hofmann H

更新日期：1998-09-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

journal_title：Journal of clinical microbiology

authors： Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

更新日期：2002-11-01 00:00:00

abstract：:Vaginal isolates of Candida albicans from human immunodeficiency virus-positive (HIV+) and HIV- women with or without candidal vaginitis were examined for secretory aspartyl proteinase (Sap) production in vitro and in vivo and for the possible correlation of Sap production with pathology and antimycotic susceptibility...

journal_title：Journal of clinical microbiology

authors： de Bernardis F,Mondello F,Scaravelli G,Pachì A,Girolamo A,Agatensi L,Cassone A

更新日期：1999-05-01 00:00:00

abstract：:Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isola...

journal_title：Journal of clinical microbiology

authors： Rodrigues ER,Ferreira AG,Leão RS,Leite CC,Carvalho-Assef AP,Albano RM,Marques EA

更新日期：2015-12-01 00:00:00

abstract：:The frequencies of 10 opportunistic DNA viruses were determined by multiplex real-time PCR in paired cerebrospinal fluid (CSF) and brain tissue of HIV-infected individuals. In the CSF, viruses were detectable in 45/55 cases: JC virus (JCV) in 62%, Epstein-Barr virus (EBV) in 44%, cytomegalovirus (CMV) in 25%, varicell...

journal_title：Journal of clinical microbiology

authors： Kannangai R,Sachithanandham J,Mahadevan A,Abraham AM,Sridharan G,Desai A,Ravi V,Shankar SK

更新日期：2013-03-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:In 1997 and 1998, H3N2 influenza A viruses emerged among pigs in North America. Genetic analyses of the H3N2 isolates demonstrated that they had distinctly different genotypes. The most commonly isolated viruses in the United States have a triple-reassortant genotype, with the hemagglutinin, neuraminidase, and PB1 pol...

journal_title：Journal of clinical microbiology

authors： Landolt GA,Karasin AI,Phillips L,Olsen CW

更新日期：2003-05-01 00:00:00

abstract：:To investigate the interaction of Limulus amoebocyte lysate (LAL) with gram-negative bacteria, soluble antigens from sonicated Pseudomonas aeruginosa were studied by various crossed-immunoelectrophoresis methods before and after reaction with LAL. Of 64 possible, at least 7 antigens were affected, as indicated by prec...

journal_title：Journal of clinical microbiology

authors： Baek L,Høiby N,Hertz JB,Espersen F

更新日期：1985-08-01 00:00:00

abstract：:The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal puls...

journal_title：Journal of clinical microbiology

authors： Morrison D,Woodford N,Barrett SP,Sisson P,Cookson BD

更新日期：1999-04-01 00:00:00

abstract：:A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

journal_title：Journal of clinical microbiology

authors： Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

更新日期：2005-08-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00

abstract：:An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...

journal_title：Journal of clinical microbiology

authors： Faulde M,Putzker M,Mertes T,Sobe D

更新日期：1991-02-01 00:00:00

abstract：:GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

journal_title：Journal of clinical microbiology

authors： Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

更新日期：1999-12-01 00:00:00

abstract：:A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotyp...

journal_title：Journal of clinical microbiology

authors： Fournier JM,Bouvet A,Mathieu D,Nato F,Boutonnier A,Gerbal R,Brunengo P,Saulnier C,Sagot N,Slizewicz B

更新日期：1993-05-01 00:00:00

abstract：:Eight Staphylococcus aureus strains initially identified by Vitek GPS-BS or GPS-SA cards as resistant to oxacillin, but susceptible to most non-beta-lactam antibiotics, were found on further testing to be susceptible to oxacillin and ceftizoxime by disk diffusion tests. For all these strains, the MICs of oxacillin wer...

journal_title：Journal of clinical microbiology

authors： Ribeiro J,Vieira FD,King T,D'Arezzo JB,Boyce JM

更新日期：1999-05-01 00:00:00

abstract：:Complement-fixing antibody was detected in 13 of 16 patients with histological and/or culture evidence of infection with Nocardia species. The antigen used was a filtrate of soluble substances secreted into liquid growth medium by cultures of N. asteroides. Apparent false positives reactions were found in three of thr...

journal_title：Journal of clinical microbiology

authors： Shainhouse JZ,Pier AC,Stevens DA

更新日期：1978-11-01 00:00:00

abstract：:Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

journal_title：Journal of clinical microbiology

authors： Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

更新日期：2012-02-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

journal_title：Journal of clinical microbiology

authors： Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

更新日期：2013-05-01 00:00:00