Interaction between limulus amoebocyte lysate and soluble antigens from Pseudomonas aeruginosa and Staphylococcus aureus studied by quantitative immunoelectrophoresis.

abstract：:DNA probes (Gen-Probe, San Diego, Calif.) directed at the Mycobacterium tuberculosis complex and Mycobacterium avium-M. intracellulare complex were used to identify acid-fast bacilli directly from specimens grown in BACTEC 12B bottles (Becton Dickinson and Co., Towson, Md.). Clinical specimens were inoculated directly...

journal_title：Journal of clinical microbiology

authors： Peterson EM,Lu R,Floyd C,Nakasone A,Friedly G,de la Maza LM

更新日期：1989-07-01 00:00:00

abstract：:The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine speci...

journal_title：Journal of clinical microbiology

authors： Boppana SB,Smith RJ,Stagno S,Britt WJ

更新日期：1992-03-01 00:00:00

abstract：:Two cases of primary invasive cutaneous infections caused by the zoophilic dermatophytic species Microsporum canis are presented. The first case occurred in a liver transplant recipient who was receiving immunosuppressive therapy. Multiple erythematous papules were seen on both legs, and a biopsy revealed invasive fun...

journal_title：Journal of clinical microbiology

authors： King D,Cheever LW,Hood A,Horn TD,Rinaldi MG,Merz WG

更新日期：1996-02-01 00:00:00

abstract：:Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Callegaro A,Scarparo C,Penati V,Nista D,Bornigia S,Lacchini C,Scagnelli M,Santini G,De Sio G

更新日期：1998-12-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for th...

journal_title：Journal of clinical microbiology

authors： Ahmed N,Mohanty AK,Mukhopadhyay U,Batish VK,Grover S

更新日期：1998-10-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:This is the first reported case of endocarditis due to the Lactobacillus-like vancomycin-resistant gram-positive bacillus Weissella confusa. Full identification and susceptibility testing of Lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that sugges...

journal_title：Journal of clinical microbiology

authors： Flaherty JD,Levett PN,Dewhirst FE,Troe TE,Warren JR,Johnson S

更新日期：2003-05-01 00:00:00

abstract：:Broth microdilution tests and an antimicrobial interaction (synergy) studies using various combinations of cefoperazone and sulbactam were performed in an effort to determine the most appropriate in vitro dilution test system. The test results with cefoperazone and sulbactam were categorized as synergistic (complete o...

journal_title：Journal of clinical microbiology

authors： Jones RN,Barry AL,Packer RR,Gregory WW,Thornsberry C

更新日期：1987-09-01 00:00:00

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for qu...

journal_title：Journal of clinical microbiology

authors： Benki S,McClelland RS,Emery S,Baeten JM,Richardson BA,Lavreys L,Mandaliya K,Overbaugh J

更新日期：2006-12-01 00:00:00

abstract：:A selective medium was developed for the isolation of Bacteroides fragilis directly from ovine and bovine fecal samples. The medium (tryptose blood agar plus polymyxin B, triclosan (Irgasan), novobiocin, and nalidixic acid) permitted growth of B. fragilis and several other species of Bacteroides but inhibited growth o...

journal_title：Journal of clinical microbiology

authors： Border M,Firehammer BD,Shoop DS,Myers LL

更新日期：1985-03-01 00:00:00

abstract：:In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged storage at room tempera...

journal_title：Journal of clinical microbiology

authors： Shima K,Wu Y,Sugimoto N,Asakura M,Nishimura K,Yamasaki S

更新日期：2006-11-01 00:00:00

abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...

journal_title：Journal of clinical microbiology

authors： Meyer MP,Eddy T,Baughn RE

更新日期：1994-03-01 00:00:00

abstract：:Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

journal_title：Journal of clinical microbiology

authors： Shibl AM,Al-Sowaygh IA

更新日期：1980-02-01 00:00:00

abstract：:A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

journal_title：Journal of clinical microbiology

authors： Fakhr MK,Logue CM

更新日期：2007-10-01 00:00:00

abstract：:To halt the human immunodeficiency virus type 1 (HIV-1) epidemic requires interventions that can prevent transmission of numerous HIV-1 subtypes. The most frequently transmitted viruses belong to the subtypes A, B, and C and the circulating recombinant forms (CRFs) AE and AG. A fast one-tube assay that identifies and ...

journal_title：Journal of clinical microbiology

authors： de Baar MP,Timmermans EC,Bakker M,de Rooij E,van Gemen B,Goudsmit J

更新日期：2001-05-01 00:00:00

abstract：:The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE

更新日期：1982-08-01 00:00:00

abstract：:Among nine patients with bacteremia caused by Granulicatella or Gemella in a 6-year period (July 1995 to June 2001), three had bacteremia caused by erythromycin-resistant Granulicatella adiacens and one had bacteremia caused by erythromycin-resistant Gemella haemolysans. All four isolates possessed mef genes, whereas ...

journal_title：Journal of clinical microbiology

authors： Woo PC,To AP,Lau SK,Fung AM,Yuen KY

更新日期：2004-07-01 00:00:00

abstract：:The taxonomic status and structure of Streptococcus dysgalactiae have been the object of much confusion. Bacteria belonging to this species are usually referred to as Lancefield group C or group G streptococci in clinical settings in spite of the fact that these terms lack precision and prevent recognition of the exac...

journal_title：Journal of clinical microbiology

authors： Jensen A,Kilian M

更新日期：2012-01-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional ...

journal_title：Journal of clinical microbiology

authors： Woods GL,Mills RD

更新日期：1988-03-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:Seventy-six nontuberculous mycobacterial isolates obtained from patients living in Greece were analyzed with the GenoType Mycobacterium CM (for common mycobacteria) and AS (for additional species) assays. GenoType correctly identified all but one of the mycobacterial species. For this species, additional probes should...

journal_title：Journal of clinical microbiology

authors： Gitti Z,Neonakis I,Fanti G,Kontos F,Maraki S,Tselentis Y

更新日期：2006-06-01 00:00:00

abstract：:We report the first case of peritonitis caused by Roseomonas mucosa which led to technique failure in an adolescent patient with HIV receiving peritoneal dialysis. Identification of the causative organism by 16S rRNA gene sequencing and phylogenetic analysis is described. ...

journal_title：Journal of clinical microbiology

authors： Boyd MA,Laurens MB,Fiorella PD,Mendley SR

更新日期：2012-11-01 00:00:00

abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

journal_title：Journal of clinical microbiology

authors： Starnbach MN,Falkow S,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Hepatitis E virus (HEV) genotype 3 is endemic in Europe and hyperendemic in southern France. Recent reports of a high prevalence of HEV RNA in blood donations and in culinary specialties from this geographical area confirmed the endemicity of HEV and sources of viral transmission in this geographical area. HEV causes ...

journal_title：Journal of clinical microbiology

authors： Moal V,Legris T,Motte A,Vacher-Coponat H,Fages L,Jourde-Chiche N,Borentain P,Jaubert D,Gerolami R,Colson P

更新日期：2015-05-01 00:00:00

abstract：:We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed b...

journal_title：Journal of clinical microbiology

authors： Pichon N,Ajzenberg D,Desnos-Ollivier M,Clavel M,Gantier JC,Labrousse F

更新日期：2008-09-01 00:00:00

abstract：:Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...

journal_title：Journal of clinical microbiology

authors： Barman P,Ghosh S,Das S,Varghese V,Chaudhuri S,Sarkar S,Krishnan T,Bhattacharya SK,Chakrabarti A,Kobayashi N,Naik TN

更新日期：2004-06-01 00:00:00