Analysis of western blotting (immunoblotting) technique in diagnosis of congenital syphilis.

abstract：:We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant resul...

journal_title：Journal of clinical microbiology

authors： Saffert RT,Cunningham SA,Ihde SM,Jobe KE,Mandrekar J,Patel R

更新日期：2011-03-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

journal_title：Journal of clinical microbiology

authors： Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

更新日期：1986-09-01 00:00:00

abstract：:Klebsiella pneumoniae has been emerging as the leading cause of liver abscess in diabetic patients. Results of molecular typing of K. pneumoniae isolates from two siblings with liver abscess, their family members, and the environment suggest a possibility of cross infection of liver abscess by the fecal-oral route wit...

journal_title：Journal of clinical microbiology

authors： Chiu CH,Su LH,Wu TL,Hung IJ

更新日期：2001-06-01 00:00:00

abstract：:Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

journal_title：Journal of clinical microbiology

authors： Tsukamura M

更新日期：1981-08-01 00:00:00

abstract：:Clonal spread and horizontal transfer in the spread of vancomycin resistance genes were investigated. Multiplex PCR, pulsed-field gel electrophoresis (PFGE), hybridization of enterococcal plasmids with the vanA and vanB probes, and sequencing of a fragment of vanB were used in the analysis. Before May 1996, 12 vancomy...

journal_title：Journal of clinical microbiology

authors： Suppola JP,Kolho E,Salmenlinna S,Tarkka E,Vuopio-Varkila J,Vaara M

更新日期：1999-12-01 00:00:00

abstract：:Preemptive ganciclovir (GCV) therapy is adopted increasingly in hematopoietic stem cell transplant (HCT) recipients, but occasional cases of increasing cytomegalovirus (CMV) antigenemia levels occur during preemptive GCV therapy. This prospective study investigated the incidence, risk factors, and clinical outcomes of...

journal_title：Journal of clinical microbiology

authors： Park SY,Lee SO,Choi SH,Kim YS,Woo JH,Sung H,Kim MN,Kim DY,Lee JH,Lee JH,Lee KH,Kim SH

更新日期：2011-12-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:"Norwalk-like viruses" (NLVs) are the most common cause of outbreaks of nonbacterial gastroenteritis worldwide. To date, the method most widely used for typing of NLV strains is sequencing and subsequent phylogenetic analysis of reverse transcription (RT)-PCR products, which has revealed the existence of stable distin...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Koopmans MP

更新日期：2000-07-01 00:00:00

abstract：:Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. W...

journal_title：Journal of clinical microbiology

authors： Lau R,Wang A,Chong-Kit A,Ralevski F,Boggild AK

更新日期：2015-04-01 00:00:00

abstract：:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses. ...

journal_title：Journal of clinical microbiology

authors： Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli M

更新日期：2001-05-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempt...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Tamura M,Asami Y,Kitamura E,Saito K,Suzuki T,Takahashi SN,Matsumoto K,Sawada S,Yokoyama E,Takasu T

更新日期：2008-05-01 00:00:00

abstract：:Bordetella bronchiseptica and toxigenic Pasteurella multocida are the etiologic agents of swine atrophic rhinitis. Methods currently used for their identification are time-consuming and suffer from a lack of sensitivity. We describe a colony lift-hybridization assay for detection of B. bronchiseptica and toxigenic P. ...

journal_title：Journal of clinical microbiology

authors： Register KB,Lee RM,Thomson C

更新日期：1998-11-01 00:00:00

abstract：:Mupirocin is a topical antimicrobial used to eradicate methicillin-resistant Staphylococcus aureus (MRSA) colonization, usually in the absence of susceptibility testing. We hypothesized that high-level (HL) mupirocin resistance was associated with multidrug resistance (MDR). To this end, unique patient isolates identi...

journal_title：Journal of clinical microbiology

authors： Cadilla A,David MZ,Daum RS,Boyle-Vavra S

更新日期：2011-01-01 00:00:00

abstract：:tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR ...

journal_title：Journal of clinical microbiology

authors： Baele M,Baele P,Vaneechoutte M,Storms V,Butaye P,Devriese LA,Verschraegen G,Gillis M,Haesebrouck F

更新日期：2000-11-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00

abstract：:Early detection of Mycobacterium tuberculosis complex (MTBC) and markers conveying drug resistance can have a beneficial impact on preventive public health actions. We describe here a new molecular point-of-care (POC) system, the Genedrive, which is based on simple sample preparation combined with PCR to detect MTBC a...

journal_title：Journal of clinical microbiology

authors： Castan P,de Pablo A,Fernández-Romero N,Rubio JM,Cobb BD,Mingorance J,Toro C

更新日期：2014-02-01 00:00:00

abstract：:A gag-based molecular beacon assay utilizing real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) subtype C (C and C') circulating in Ethiopia. Of 41 samples, 36 could be classified as C or ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Pollakis G,Abebe A,Fisseha B,Tegbaru B,Tesfaye G,Mengistu Y,Wolday D,van Gemen B,Goudsmit J,Dorigo-Zetsma W,de Baar MP

更新日期：2004-04-01 00:00:00

abstract：:An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

journal_title：Journal of clinical microbiology

authors： Barreau C,Wagener G

更新日期：1990-08-01 00:00:00

abstract：:Until now, serologic tests that distinguish the closely related human T-cell lymphotropic virus types I (HTLV-I) and II (HTLV-II) infections have not been available. Synthetic peptide assays, employing peptides derived from the core and envelope proteins of HTLV-I and HTLV-II (SynthEIA and Select-HTLV tests), were eva...

journal_title：Journal of clinical microbiology

authors： Lal RB,Heneine W,Rudolph DL,Present WB,Hofhienz D,Hartley TM,Khabbaz RF,Kaplan JE

更新日期：1991-10-01 00:00:00

abstract：:The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmission is overestima...

journal_title：Journal of clinical microbiology

authors： Sloot R,Borgdorff MW,de Beer JL,van Ingen J,Supply P,van Soolingen D

更新日期：2013-07-01 00:00:00

abstract：:A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

journal_title：Journal of clinical microbiology

authors： Holmberg O,Lyon RH,Price D,Farnsworth RJ

更新日期：1979-09-01 00:00:00

abstract：:Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...

journal_title：Journal of clinical microbiology

authors： Soria ME,García-Crespo C,Martínez-González B,Vázquez-Sirvent L,Lobo-Vega R,de Ávila AI,Gallego I,Chen Q,García-Cehic D,Llorens-Revull M,Briones C,Gómez J,Ferrer-Orta C,Verdaguer N,Gregori J,Rodríguez-Frías F,Buti M,Este

更新日期：2020-11-18 00:00:00

abstract：:DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an ind...

journal_title：Journal of clinical microbiology

authors： Yu XJ,Crocquet-Valdes P,Cullman LC,Walker DH

更新日期：1996-11-01 00:00:00

abstract：:A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

journal_title：Journal of clinical microbiology

authors： Krieg DP,Bass JA,Mattingly SJ

更新日期：1986-12-01 00:00:00

abstract：:Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that...

journal_title：Journal of clinical microbiology

authors： Jou NT,Yoshimori RB,Mason GR,Louie JS,Liebling MR

更新日期：1997-05-01 00:00:00

abstract：:The sensitivity and specificity of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay (BED-CEIA) were compared with those of the avidity index method to identify recent HIV infection using a panel of 148 samples (81 patients) representing durations of infe...

journal_title：Journal of clinical microbiology

authors： Loschen S,Bätzing-Feigenbaum J,Poggensee G,Cordes C,Hintsche B,Rausch M,Dupke S,Gohlke-Micknis S,Rödig J,Hamouda O,Kücherer C

更新日期：2008-01-01 00:00:00

abstract：:The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyac...

journal_title：Journal of clinical microbiology

authors： Binko BP,Bartholomew WR

更新日期：1978-08-01 00:00:00