Relationship between photochromogenicity and test temperature in mycobacteria.

abstract：:Tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen Francisella tularensis. Despite the monomorphic nature of sequenced F. tularensis genomes, there is a significant degree of plasticity in the organization of genetic elements. The observed variability in these genome...

journal_title：Journal of clinical microbiology

authors： Larson MA,Fey PD,Bartling AM,Iwen PC,Dempsey MP,Francesconi SC,Hinrichs SH

更新日期：2011-08-01 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...

journal_title：Journal of clinical microbiology

authors： Effelsberg N,Stegger M,Peitzmann L,Altinok O,Coombs GW,Pichon B,Kearns A,Randad PR,Heaney CD,Bletz S,Schaumburg F,Mellmann A

更新日期：2020-12-17 00:00:00

abstract：:To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

journal_title：Journal of clinical microbiology

authors： Dimitrov DH,Melnick JL,Hollinger FB

更新日期：1990-04-01 00:00:00

abstract：:A total of 410 well-defined Campylobacter, Helicobacter, and Wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the Centers for Disease Control, Atlanta, Ga. All C. coli (43 strains), C. cryaerophila (34...

journal_title：Journal of clinical microbiology

authors： Popovic-Uroic T,Patton CM,Nicholson MA,Kiehlbauch JA

更新日期：1990-10-01 00:00:00

abstract：:A variety of bacterins, vaccines, and antisera retained greater than 90% of their original level of mercurial preservative after lyophilization, and this might influence certain uses of these products. ...

journal_title：Journal of clinical microbiology

authors： Pemberton JR

更新日期：1975-12-01 00:00:00

abstract：:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex of bacteria. This species was originally identified as a pathogen of small rodents and shrews and was associated with limited diversity and a much reduced spoligotype pattern. More recently, specific deletions of chromosomal DNA have been show...

journal_title：Journal of clinical microbiology

authors： Smith NH,Crawshaw T,Parry J,Birtles RJ

更新日期：2009-08-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:Relative amounts of D-arabinitol (fungal origin) and L-arabinitol (part of normal human metabolism) in urine were determined by gas chromatography and mass spectrometry from 61 hospitalized patients with hematological malignancies. Seventeen neutropenic patients with acute leukemia (with 53 samples) had disseminated y...

journal_title：Journal of clinical microbiology

authors： Lehtonen L,Anttila VJ,Ruutu T,Salonen J,Nikoskelainen J,Eerola E,Ruutu P

更新日期：1996-09-01 00:00:00

abstract：:Community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including Southeast Asia. Active surveillance for bloodstream infections has been conducted i...

journal_title：Journal of clinical microbiology

authors： Jorakate P,Higdon M,Kaewpan A,Makprasert S,Yuenprakhon S,Tawisaid K,Dejsirilert S,Whistler T,Baggett HC

更新日期：2015-03-01 00:00:00

abstract：:A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients wi...

journal_title：Journal of clinical microbiology

authors： Lee JC,Liu MJ,Parsonnet J,Arbeit RD

更新日期：1990-12-01 00:00:00

abstract：:Results of serotyping 966 clinical isolates of Pseudomonas aeruginosa showed that 72% agglutinated specifically in one or another of the 16 typing antisera, but 28% agglutinated in two or more and often in as many as 10 antisera; this polyagglutinability correlated with a high incidence of cross-reactivity among the a...

journal_title：Journal of clinical microbiology

authors： Duncan NH,Hinton NA,Penner JL,Duncan IB

更新日期：1976-08-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...

journal_title：Journal of clinical microbiology

authors： Reijans M,Dingemans G,Klaassen CH,Meis JF,Keijdener J,Mulders B,Eadie K,van Leeuwen W,van Belkum A,Horrevorts AM,Simons G

更新日期：2008-04-01 00:00:00

abstract：:The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of ...

journal_title：Journal of clinical microbiology

authors： Bell-Sakyi L,Paxton EA,Munderloh UG,Sumption KJ

更新日期：2000-03-01 00:00:00

abstract：:A panel of new cytomegalovirus (CMV) assays for use on the Architect instrument has been developed, including a CMV avidity assay based on a new technology. The purpose of this study was to compare the performance characteristics of the fully automated CMV immunoglobulin M (IgM), IgG, and IgG avidity tests on the Arch...

journal_title：Journal of clinical microbiology

authors： Lagrou K,Bodeus M,Van Ranst M,Goubau P

更新日期：2009-06-01 00:00:00

abstract：:We have developed a PCR assay for one-step differentiation of the three complexes of New World Leishmania (Leishmania braziliensis, Leishmania mexicana, and Leishmania donovani). This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these organisms and can detect all three complexes si...

journal_title：Journal of clinical microbiology

authors： Harris E,Kropp G,Belli A,Rodriguez B,Agabian N

更新日期：1998-07-01 00:00:00

abstract：:The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE

更新日期：1982-08-01 00:00:00

abstract：:Prototheca spp. are environmental algae that may cause serious infection in the immunocompromised patient. Clinical manifestations may mimic other diseases. We present a case of fatal infection in a 78-year-old cardiac transplant recipient and discuss pitfalls in the clinical and laboratory diagnoses. ...

journal_title：Journal of clinical microbiology

authors： McMullan B,Muthiah K,Stark D,Lee L,Marriott D

更新日期：2011-08-01 00:00:00

abstract：:The prevalence of Leishmania infantum-specific antibodies and asymptomatic infection was assessed in a randomized sample of 526 healthy adults from a continental area of northwestern Italy where L. infantum is not endemic and where autochthonous cases of visceral leishmaniasis (VL) were recently reported. L. infantum-...

journal_title：Journal of clinical microbiology

authors： Biglino A,Bolla C,Concialdi E,Trisciuoglio A,Romano A,Ferroglio E

更新日期：2010-01-01 00:00:00

abstract：:The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal puls...

journal_title：Journal of clinical microbiology

authors： Morrison D,Woodford N,Barrett SP,Sisson P,Cookson BD

更新日期：1999-04-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was pos...

journal_title：Journal of clinical microbiology

authors： Díaz R,Maraví-Poma E,Delgado G,Rivero A

更新日期：1978-02-01 00:00:00

abstract：:We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...

journal_title：Journal of clinical microbiology

authors： Ong DC,Yam WC,Siu GK,Lee AS

更新日期：2010-04-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:The usefulness of Candida ID 2 (CAID2) reformulated medium (bioMérieux, France) has been compared with that of the former Candida ID (CAID; bioMérieux), Albicans ID 2 (ALB2; bioMérieux), and CHROMagar Candida (CAC; Chromagar, France) chromogenic media for the isolation and presumptive identification of clinically rele...

journal_title：Journal of clinical microbiology

authors： Eraso E,Moragues MD,Villar-Vidal M,Sahand IH,González-Gómez N,Pontón J,Quindós G

更新日期：2006-09-01 00:00:00

abstract：:Fifty-six strains of Borrelia burgdorferi sensu lato, isolated from ticks and vertebrate animals in Missouri, South Carolina, Georgia, Florida, and Texas, were identified and characterized by PCR-restriction fragment length polymorphism (RFLP) analysis of rrf (5S)-rrl (23S) intergenic spacer amplicons. A total of 241 ...

journal_title：Journal of clinical microbiology

authors： Lin T,Oliver JH Jr,Gao L,Kollars TM Jr,Clark KL

更新日期：2001-07-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00