Two-color hybridization assay for simultaneous detection of Bordetella bronchiseptica and toxigenic Pasteurella multocida from swine.

abstract：:The R/B and Minitek systems for the identification of Enterobacteriaceae were evaluated, and the results were compared with those obtained by conventional methods. Both systems were rapid and allowed correct identification of about 85% of the 294 isolates (23 species) examined. The individual biochemical reactions sho...

journal_title：Journal of clinical microbiology

authors： Shayegani M,Hubbard ME,Hiscott T,McGlynn D

更新日期：1975-06-01 00:00:00

abstract：:New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...

journal_title：Journal of clinical microbiology

authors： Huang L,Hu X,Zhou M,Yang Y,Qiao J,Wang D,Yu J,Cui Z,Zhang Z,Zhang XE,Wei H

更新日期：2014-06-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

journal_title：Journal of clinical microbiology

authors： Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

更新日期：2003-05-01 00:00:00

abstract：:We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Myco...

journal_title：Journal of clinical microbiology

authors： Bencina D,Bradbury JM

更新日期：1992-02-01 00:00:00

abstract：:Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since ...

journal_title：Journal of clinical microbiology

authors： Rónai Z,Csivincsik Á,Dán Á

更新日期：2015-05-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typ...

journal_title：Journal of clinical microbiology

authors： Kremer K,van Soolingen D,Frothingham R,Haas WH,Hermans PW,Martín C,Palittapongarnpim P,Plikaytis BB,Riley LW,Yakrus MA,Musser JM,van Embden JD

更新日期：1999-08-01 00:00:00

abstract：:A simplified procedure for serotyping Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. The assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assa...

journal_title：Journal of clinical microbiology

authors： Mills SD,Congi RV,Hennessy JN,Penner JL

更新日期：1991-10-01 00:00:00

abstract：:In this study, 102 herpes simplex virus isolates were typed by cell culture selection (chicken embryo cells and guinea pig embryo cells [CE/GPE]), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) sensitivity, plaque reduction neutralization, and indirect immunofluorescence staining techniques. The percentages of agreement ...

journal_title：Journal of clinical microbiology

authors： Zheng ZM,Mayo DR,Hsiung GD

更新日期：1983-03-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:Nucleic acid tests are sensitive and specific and provide a rapid diagnosis, making them invaluable for patient and outbreak management. Multiplex PCR assays have additional advantages in providing an economical and comprehensive panel for many common respiratory viruses. Previous reports have shown the utility of the...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong S,Tokaryk KL,Fonseca K,Drews SJ

更新日期：2011-05-01 00:00:00

abstract：:We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favor...

journal_title：Journal of clinical microbiology

authors： Meyers PR,Bourn WR,Steyn LM,van Helden PD,Beyers AD,Brown GD

更新日期：1998-09-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00

abstract：:We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the v...

journal_title：Journal of clinical microbiology

authors： Petraitiene R,Petraitis V,Witt JR 3rd,Durkin MM,Bacher JD,Wheat LJ,Walsh TJ

更新日期：2011-12-01 00:00:00

abstract：:Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16...

journal_title：Journal of clinical microbiology

authors： Kostman JR,Edlind TD,LiPuma JJ,Stull TL

更新日期：1992-08-01 00:00:00

abstract：:Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

journal_title：Journal of clinical microbiology

authors： Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

更新日期：2005-02-01 00:00:00

abstract：:A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) we...

journal_title：Journal of clinical microbiology

authors： Tang X,Zhao Z,Hu J,Wu B,Cai X,He Q,Chen H

更新日期：2009-04-01 00:00:00

abstract：:A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...

journal_title：Journal of clinical microbiology

authors： Bai GH,Kim SJ,Chang CL,National and Regional Tuberculosis Reference Laboratories.

更新日期：2007-11-01 00:00:00

abstract：:The Vitek MS v2.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system accurately distinguished Streptococcus pneumoniae from nonpneumococcal S. mitis group species. Only 1 of 116 nonpneumococcal isolates (<1%) was misidentified as S. pneumoniae. None of 95 pneumococcal isolates was misi...

journal_title：Journal of clinical microbiology

authors： Branda JA,Markham RP,Garner CD,Rychert JA,Ferraro MJ

更新日期：2013-09-01 00:00:00

abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

journal_title：Journal of clinical microbiology

authors： Martinez LR,Garcia-Rivera J,Casadevall A

更新日期：2001-09-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

journal_title：Journal of clinical microbiology

authors： Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

更新日期：2003-02-01 00:00:00

abstract：:The cobas human papillomavirus (HPV) test, approved by the FDA in April 2011, is a fully automated assay for the detection of 14 high-risk (hr) HPV genotypes from cervical specimens collected in liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes. Results are simultaneously ...

journal_title：Journal of clinical microbiology

authors： Rao A,Young S,Erlich H,Boyle S,Krevolin M,Sun R,Apple R,Behrens C

更新日期：2013-05-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease. ...

journal_title：Journal of clinical microbiology

authors： McCarthy LR,Carlson JR

更新日期：1981-12-01 00:00:00