Association of high-level mupirocin resistance and multidrug-resistant methicillin-resistant Staphylococcus aureus at an academic center in the midwestern United States.

abstract：:During a taxonomic investigation of Nocardia spp. and Streptomyces spp., we received 658 isolates from laboratories of both human and veterinary medicine. Our procedure leading to the identification of 92% of these isolates, the species that they represented, and a characteristic pattern of properties of the strains o...

journal_title：Journal of clinical microbiology

authors： Mishra SK,Gordon RE,Barnett DA

更新日期：1980-06-01 00:00:00

abstract：:The COVID-19 pandemic has put the clinical laboratory in the spotlight. The news media is regularly seeking out interviews with microbiologists, infectious disease specialists, and pathologists. Increased public exposure offers opportunities to improve how laboratory professionals communicate our insights. We can emph...

journal_title：Journal of clinical microbiology

authors： Mazer BL

更新日期：2021-01-21 00:00:00

abstract：:The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmission is overestima...

journal_title：Journal of clinical microbiology

authors： Sloot R,Borgdorff MW,de Beer JL,van Ingen J,Supply P,van Soolingen D

更新日期：2013-07-01 00:00:00

abstract：:During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A meas...

journal_title：Journal of clinical microbiology

authors： Roy F,Mendoza L,Hiebert J,McNall RJ,Bankamp B,Connolly S,Lüdde A,Friedrich N,Mankertz A,Rota PA,Severini A

更新日期：2017-03-01 00:00:00

abstract：:An outbreak of pseudobacteremia due to Streptococcus pyogenes (group A streptococci [GAS]) and methicillin-susceptible Staphylococcus aureus (MSSA) was traced to the venting procedure for aerobic bottles prior to their loading into the incubator of the BacT/Alert analyzer (Organon Teknika). Bacteria shed by a laborato...

journal_title：Journal of clinical microbiology

authors： Simhon A,Rahav G,Shapiro M,Block C

更新日期：2001-01-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from genome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), th...

journal_title：Journal of clinical microbiology

authors： Aradaib IE,Wilson WC,Schore CE,Mohammed ME,Yilma TD,Cullor JS,Osburn BI

更新日期：1998-09-01 00:00:00

abstract：:Clinical evidence for failure with beta-lactam therapy has been lacking for patients with borderline oxacillin-resistant Staphylococcus aureus (BORSA) infections. We describe a failure of cloxacillin for a patient with endocarditis due to BORSA. The isolate also had false-negative thermonuclease and coagulase test res...

journal_title：Journal of clinical microbiology

authors： Skinner S,Murray M,Walus T,Karlowsky JA

更新日期：2009-03-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...

journal_title：Journal of clinical microbiology

authors： Buller RS,Bailey TC,Ettinger NA,Keener M,Langlois T,Miller JP,Storch GA

更新日期：1992-10-01 00:00:00

abstract：:A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization in Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal colonization incidence of 7.4%. This is a follow-up study to determine the current prevalence of MRSA colonization and to further characterize th...

journal_title：Journal of clinical microbiology

authors： Al-Rawahi GN,Schreader AG,Porter SD,Roscoe DL,Gustafson R,Bryce EA

更新日期：2008-02-01 00:00:00

abstract：:CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S r...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Steigerwalt AG,O'Connor SP,Hollis DG,Weyant RS,Weaver RE,Brenner DJ

更新日期：1993-09-01 00:00:00

abstract：:A study was conducted to compare three automated systems and the Gram stain for their ability to detect significant bacteriuria. A total of 1,000 urine specimens were evaluated by Autobac MTS (General Diagnostics), Auto Microbic system (AMS; Vitek Systems, Inc.), and MS-2 (Abbott Laboratories) and compared with a semi...

journal_title：Journal of clinical microbiology

authors： Pezzlo MT,Tan GL,Peterson EM,de la Maza LM

更新日期：1982-03-01 00:00:00

abstract：:Rhodococcus equi infection is increasing in regions with high HIV prevalence worldwide. The microbiological features and clinical mimicry of tuberculosis infection pose diagnostic challenges in high-tuberculosis-incidence settings. We present two HIV-associated cases of R. equi infection from Vietnam and discuss the u...

journal_title：Journal of clinical microbiology

authors： Le T,Cash-Goldwasser S,Tho PV,Lan NP,Campbell JI,van Doorn HR,Lam NT,Trung NV,Trinh DT,Van Kinh N,Wertheim HF

更新日期：2015-04-01 00:00:00

abstract：:The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the National Committee for Clinical Laboratory Standards (NCCLS) approved standard broth microdilution (BMD) method. The NCCLS met...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Boyken L,Messer SA,Tendolkar S,Hollis RJ

更新日期：2003-05-01 00:00:00

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:Six patients with bone marrow cultures yielding Mycobacterium avium complex were encountered at the Mount Sinai Hospital between 1969 and 1976. One additional isolate of the same mycobacterial species was recovered from splenic cyst fluid of a seventh patient. Because none of the patients had illnesses apparently due ...

journal_title：Journal of clinical microbiology

authors： Kozinn WP,Damsker B,Bottone EJ

更新日期：1980-03-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

journal_title：Journal of clinical microbiology

authors： Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

更新日期：1986-08-01 00:00:00

abstract：:Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Unicomb LE,Bishop RF

更新日期：1987-01-01 00:00:00

abstract：:Cases of invasive mycosis due to Blastobotrys serpentis and B. proliferans identified by sequencing in a preterm patient and a rhabdomyosarcoma patient, respectively, are reported. Both species revealed elevated fluconazole and echinocandin MICs by the CLSI broth microdilution method. Additionally, B. serpentis exhibi...

journal_title：Journal of clinical microbiology

authors： Kumar A,Babu R,Bijulal S,Abraham M,Sasidharan P,Kathuria S,Sharma C,Meis JF,Chowdhary A

更新日期：2014-11-01 00:00:00

abstract：:In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Kettlitz C,Werner G,Witte W

更新日期：2003-09-01 00:00:00

abstract：:Rothia spp. are Gram-positive cocco-bacilli that cause a wide range of serious infections, especially in immunocompromised hosts. Risk factors for Rothia mucilaginosa (previously known as Stomatococcus mucilaginosus) bacteremia include prolonged and profound neutropenia, malignancy, and an indwelling vascular foreign ...

journal_title：Journal of clinical microbiology

authors： Ramanan P,Barreto JN,Osmon DR,Tosh PK

更新日期：2014-09-01 00:00:00

abstract：:A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance for establishing whether BSE has entered the sheep population. Since BSE has not yet been found in sheep at the farm level, such discrimination procedures can be developed only with experimental sheep BS...

journal_title：Journal of clinical microbiology

authors： Thuring CM,Erkens JH,Jacobs JG,Bossers A,Van Keulen LJ,Garssen GJ,Van Zijderveld FG,Ryder SJ,Groschup MH,Sweeney T,Langeveld JP

更新日期：2004-03-01 00:00:00

abstract：:A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

journal_title：Journal of clinical microbiology

authors： Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

更新日期：2005-08-01 00:00:00

abstract：:A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

journal_title：Journal of clinical microbiology

authors： Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

更新日期：2017-12-01 00:00:00

abstract：:Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae. The probe for M. tuberculosis was prepared from Mycobacterium bovis BCG, which has been shown to be closely related to M. tuberculosis. A probe for the M. avium c...

journal_title：Journal of clinical microbiology

authors： Roberts MC,McMillan C,Coyle MB

更新日期：1987-07-01 00:00:00