Validation of the INNO-LIA syphilis kit as a confirmatory assay for Treponema pallidum antibodies.

abstract：:Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

journal_title：Journal of clinical microbiology

authors： Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

更新日期：2005-02-01 00:00:00

abstract：:Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal...

journal_title：Journal of clinical microbiology

authors： Jones KF,Norcross NL

更新日期：1983-05-01 00:00:00

abstract：:Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

journal_title：Journal of clinical microbiology

authors： Halliday CL,Carter DA

更新日期：2003-02-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:The dimorphic fungus, Penicillium marneffei, is an emerging opportunistic pathogen endemic in Southeast Asia, especially for those with impaired cellular immunity such as human immunodeficiency virus-infected persons. A discriminatory and reproducible method based on the analysis of nucleotide sequences would facilita...

journal_title：Journal of clinical microbiology

authors： Lasker BA

更新日期：2006-09-01 00:00:00

abstract：:A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clini...

journal_title：Journal of clinical microbiology

authors： Pouletty P,Chomel JJ,Thouvenot D,Catalan F,Rabillon V,Kadouche J

更新日期：1987-05-01 00:00:00

abstract：:Three cloned segments of Mycobacterium tuberculosis DNA which are promising as clinical probes were identified. An MboI digest of DNA from a clinical isolate of M. tuberculosis was cloned into bacteriophage M13. To identify recombinants specific for the M. tuberculosis complex, plaque lifts were hybridized with M. bov...

journal_title：Journal of clinical microbiology

authors： Eisenach KD,Crawford JT,Bates JH

更新日期：1988-11-01 00:00:00

abstract：:A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-l...

journal_title：Journal of clinical microbiology

authors： Lin TM,Halbert SP,Chiu CT,Zarco R

更新日期：1981-04-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:Rothia spp. are Gram-positive cocco-bacilli that cause a wide range of serious infections, especially in immunocompromised hosts. Risk factors for Rothia mucilaginosa (previously known as Stomatococcus mucilaginosus) bacteremia include prolonged and profound neutropenia, malignancy, and an indwelling vascular foreign ...

journal_title：Journal of clinical microbiology

authors： Ramanan P,Barreto JN,Osmon DR,Tosh PK

更新日期：2014-09-01 00:00:00

abstract：:The BACTEC 9240 (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) is a new continuous-monitoring blood culture system that uses internal, fluorescent-CO2 sensors. In a multicenter clinical trial, organism yield and times to detection with the prototype BACTEC 9240 system were compared with those of the BAC...

journal_title：Journal of clinical microbiology

authors： Nolte FS,Williams JM,Jerris RC,Morello JA,Leitch CD,Matushek S,Schwabe LD,Dorigan F,Kocka FE

更新日期：1993-03-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:An unusual Enterocytozoon bieneusi genotype was found in seven guinea pigs and a 2-year-old child in the same household. The genetic uniqueness of the parasite, its wide occurrence in other guinea pigs in the community, and its absence in other children in the community suggest the possibility of zoonotic transmission...

journal_title：Journal of clinical microbiology

authors： Cama VA,Pearson J,Cabrera L,Pacheco L,Gilman R,Meyer S,Ortega Y,Xiao L

更新日期：2007-08-01 00:00:00

abstract：:Pulmonary infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa toxin is one of several proposed virulence factors which may be responsible for chronic P. aeruginosa infections in these patients. With a highly specific, sensitive, and quantita...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Nowak NA,Rich CM,Braverman LE,Fischer RA

更新日期：1983-09-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00

abstract：:Restriction endonucleases are bacterial enzymes that cleave DNA at specific sites. The resulting DNA fragments may be separated electrophoretically in gel to form specific restriction patterns. In the present study, the restriction endonuclease method was successfully adapted to the analysis of the chromosomal DNA of ...

journal_title：Journal of clinical microbiology

authors： Bjorvatn B,Lund V,Kristiansen BE,Korsnes L,Spanne O,Lindqvist B

更新日期：1984-06-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00

abstract：:Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for a...

journal_title：Journal of clinical microbiology

authors： Vijayachari P,Ahmed N,Sugunan AP,Ghousunnissa S,Rao KR,Hasnain SE,Sehgal SC

更新日期：2004-08-01 00:00:00

abstract：:In an epidemiological study, nine isolates of dematiaceous fungi were recovered from the interior of a local feed and seed warehouse. Sample sites include brick walls and floors. Air samples also were included. Samples were collected in saline and plated on Mycobiotic and Sabouraud agar. The nine dematiaceous fungi re...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Kerkering TM,Shadomy HJ

更新日期：1982-04-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...

journal_title：Journal of clinical microbiology

authors： Singh DV,Isac SR,Colwell RR

更新日期：2002-11-01 00:00:00

abstract：:The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus. DNA was isolat...

journal_title：Journal of clinical microbiology

authors： Huijsdens XW,Linskens RK,Mak M,Meuwissen SG,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2002-12-01 00:00:00

abstract：:Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagen isolates from a heifer-raising operation and from 11 dairy herds that had their calves contracted to the heifer-raising operation were examined for their phenotypic and genotypic characteristics. Results of the study showed that the heifer-raising...

journal_title：Journal of clinical microbiology

authors： Hegde NV,Cook ML,Wolfgang DR,Love BC,Maddox CC,Jayarao BM

更新日期：2005-08-01 00:00:00

abstract：:The studies reported here were undertaken to assess the ability of the outer membrane proteins (OMPs) of Salmonella typhi to induce a humoral immune response in humans with typhoid fever. OMPs were isolated with the nonionic detergent Triton X-100 and were found to be contaminated with approximately 4% lipopolysacchar...

journal_title：Journal of clinical microbiology

authors： Ortiz V,Isibasi A,García-Ortigoza E,Kumate J

更新日期：1989-07-01 00:00:00

abstract：:A total of 410 well-defined Campylobacter, Helicobacter, and Wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the Centers for Disease Control, Atlanta, Ga. All C. coli (43 strains), C. cryaerophila (34...

journal_title：Journal of clinical microbiology

authors： Popovic-Uroic T,Patton CM,Nicholson MA,Kiehlbauch JA

更新日期：1990-10-01 00:00:00

abstract：:Vaginal isolates of Candida albicans from human immunodeficiency virus-positive (HIV+) and HIV- women with or without candidal vaginitis were examined for secretory aspartyl proteinase (Sap) production in vitro and in vivo and for the possible correlation of Sap production with pathology and antimycotic susceptibility...

journal_title：Journal of clinical microbiology

authors： de Bernardis F,Mondello F,Scaravelli G,Pachì A,Girolamo A,Agatensi L,Cassone A

更新日期：1999-05-01 00:00:00